Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas
International audience ex determination is poorly known in mollusks, lophotrochozoa and most hermaphrodites. In the oyster Crassostrea gigas, an irregular successive hermaphrodite, sex determination may occur at the end of a gametogenetic cycle to prepare the next cycle. To investigate further into...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Online Access: | https://hal-normandie-univ.archives-ouvertes.fr/hal-02296496 https://doi.org/10.1016/j.cbpa.2013.09.018 |
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ftccsdartic:oai:HAL:hal-02296496v1 2023-05-15T15:58:06+02:00 Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas Santerre, Christelle Sourdaine, Pascal Adeline, Béatrice Martinez, Anne-Sophie Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2014-01 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296496 https://doi.org/10.1016/j.cbpa.2013.09.018 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2013.09.018 hal-02296496 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296496 doi:10.1016/j.cbpa.2013.09.018 ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal-normandie-univ.archives-ouvertes.fr/hal-02296496 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2014, 167, pp.68-76. ⟨10.1016/j.cbpa.2013.09.018⟩ Sox9 β-catenin Sex-determining pathways Oyster Gonadic differentiation [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2014 ftccsdartic https://doi.org/10.1016/j.cbpa.2013.09.018 2021-11-21T01:16:33Z International audience ex determination is poorly known in mollusks, lophotrochozoa and most hermaphrodites. In the oyster Crassostrea gigas, an irregular successive hermaphrodite, sex determination may occur at the end of a gametogenetic cycle to prepare the next cycle. To investigate further into these aspects we have focused on orthologs of SoxE and β-catenin, key players of the male and female gonadic differentiation respectively in mammals. Based on phylogenetic analyses, Cg-SoxE, the oyster's SoxE ortholog, is closely related to vertebrate SoxE genes while Cg-β-catenin, the oyster's β-catenin ortholog, is classified amongst lophotrochozoa. The mRNA expression measured by qPCR in the gonadic area during a gametogenetic cycle is maximal for Cg-SoxE when sex is indiscernible and for Cg-β-catenin in mature females. Both expressions are localized from early germ cells to spermatocytes and pre-vitellogenic oocytes, and potentially in somatic cells. Cg-β-catenin is also expressed in vitellogenic oocytes. These actors may be involved in early oyster gonadic differentiation, which includes sex determination. Our results enhance the understanding of sex determination in C. gigas and in mollusks and they provide additional knowledge in compared genomics of reproduction and in molecular phylogeny. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 167 68 76 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Sox9 β-catenin Sex-determining pathways Oyster Gonadic differentiation [SDV]Life Sciences [q-bio] |
spellingShingle |
Sox9 β-catenin Sex-determining pathways Oyster Gonadic differentiation [SDV]Life Sciences [q-bio] Santerre, Christelle Sourdaine, Pascal Adeline, Béatrice Martinez, Anne-Sophie Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas |
topic_facet |
Sox9 β-catenin Sex-determining pathways Oyster Gonadic differentiation [SDV]Life Sciences [q-bio] |
description |
International audience ex determination is poorly known in mollusks, lophotrochozoa and most hermaphrodites. In the oyster Crassostrea gigas, an irregular successive hermaphrodite, sex determination may occur at the end of a gametogenetic cycle to prepare the next cycle. To investigate further into these aspects we have focused on orthologs of SoxE and β-catenin, key players of the male and female gonadic differentiation respectively in mammals. Based on phylogenetic analyses, Cg-SoxE, the oyster's SoxE ortholog, is closely related to vertebrate SoxE genes while Cg-β-catenin, the oyster's β-catenin ortholog, is classified amongst lophotrochozoa. The mRNA expression measured by qPCR in the gonadic area during a gametogenetic cycle is maximal for Cg-SoxE when sex is indiscernible and for Cg-β-catenin in mature females. Both expressions are localized from early germ cells to spermatocytes and pre-vitellogenic oocytes, and potentially in somatic cells. Cg-β-catenin is also expressed in vitellogenic oocytes. These actors may be involved in early oyster gonadic differentiation, which includes sex determination. Our results enhance the understanding of sex determination in C. gigas and in mollusks and they provide additional knowledge in compared genomics of reproduction and in molecular phylogeny. |
author2 |
Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Santerre, Christelle Sourdaine, Pascal Adeline, Béatrice Martinez, Anne-Sophie |
author_facet |
Santerre, Christelle Sourdaine, Pascal Adeline, Béatrice Martinez, Anne-Sophie |
author_sort |
Santerre, Christelle |
title |
Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_short |
Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_full |
Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_fullStr |
Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Cg-SoxE and Cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the Pacific oyster Crassostrea gigas |
title_sort |
cg-soxe and cg-β-catenin, two new potential actors of the sex-determining pathway in a hermaphrodite lophotrochozoan, the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-02296496 https://doi.org/10.1016/j.cbpa.2013.09.018 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal-normandie-univ.archives-ouvertes.fr/hal-02296496 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2014, 167, pp.68-76. ⟨10.1016/j.cbpa.2013.09.018⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2013.09.018 hal-02296496 https://hal-normandie-univ.archives-ouvertes.fr/hal-02296496 doi:10.1016/j.cbpa.2013.09.018 |
op_doi |
https://doi.org/10.1016/j.cbpa.2013.09.018 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
167 |
container_start_page |
68 |
op_container_end_page |
76 |
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