Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci
International audience The Montagu’s harrier (Circus pygargus) is a semi-colonial raptor species widely but patchily distributed across the Palearctic region with recorded cases of philopatry and presence of extra-pair copulation. In order to assess Montagu’s harrier spatial genetic structure and co...
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ftccsdartic:oai:HAL:hal-02108814v1 2023-05-15T15:55:33+02:00 Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci Ribout, C. Villers, A. Ruault, S. Bretagnolle, V. Picard, D. Monceau, K. Gauffre, Bertrand Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) LTSER «Zone Atelier Plaine & Val de Sevre» France Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de La Rochelle (ULR) Département de Biologie UFR Sciences - Université d'Angers France Université d'Angers (UA) 2019-02 https://hal.archives-ouvertes.fr/hal-02108814 https://doi.org/10.1007/s10709-019-00053-7 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10709-019-00053-7 info:eu-repo/semantics/altIdentifier/pmid/30671744 hal-02108814 https://hal.archives-ouvertes.fr/hal-02108814 doi:10.1007/s10709-019-00053-7 PRODINRA: 458309 WOS: 000460839000008 PUBMED: 30671744 ISSN: 0016-6707 EISSN: 1573-6857 Genetica https://hal.archives-ouvertes.fr/hal-02108814 Genetica, Springer Verlag, 2019, 147 (1), pp.69-78. ⟨10.1007/s10709-019-00053-7⟩ Raptor Spatial genetic structure Circus aeruginosus Circus cyaneus Circus pygargus Microsatellites Relatedness [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2019 ftccsdartic https://doi.org/10.1007/s10709-019-00053-7 2021-11-07T02:00:56Z International audience The Montagu’s harrier (Circus pygargus) is a semi-colonial raptor species widely but patchily distributed across the Palearctic region with recorded cases of philopatry and presence of extra-pair copulation. In order to assess Montagu’s harrier spatial genetic structure and contemporary gene flow, we developed 16 new microsatellite markers using 454 pyrosequencing. Genotypes of 117 chicks sampled in a 200 × 300 km farmland area in Central Western France were analyzed to characterize genetic polymorphism at each locus and regional and fine-scale genetic structure. Fourteen markers were found polymorphic, with a number of alleles ranging from 3 to 11. The expected and observed heterozygosities ranged from 0.36 to 0.856 and from 0.35 to 0.868, respectively. A single genetic unit was found at the regional scale with higher genetic similarity observed at a small spatial scale (up to 10 km). Our results are consistent with overall large-scale juvenile and adult dispersal together with small-scale male philopatry. Cross-species amplification of this set of microsatellites makers has been successful in two closely related harrier species: the marsh harrier (Circus aeruginosus) and the Hen harrier (Circus cyaneus) for which 14 and 12 markers were polymorphic, respectively. These new microsatellite markers could be used to study the population genetic structure, contemporary gene flow and parentage analyses in these three species and to conduct microsatellite-based demographic inferences on the Montagu’s harrier. Article in Journal/Newspaper Circus cyaneus Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Hen ENVELOPE(-64.914,-64.914,61.317,61.317) Genetica 147 1 69 78 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Raptor Spatial genetic structure Circus aeruginosus Circus cyaneus Circus pygargus Microsatellites Relatedness [SDE]Environmental Sciences |
spellingShingle |
Raptor Spatial genetic structure Circus aeruginosus Circus cyaneus Circus pygargus Microsatellites Relatedness [SDE]Environmental Sciences Ribout, C. Villers, A. Ruault, S. Bretagnolle, V. Picard, D. Monceau, K. Gauffre, Bertrand Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
topic_facet |
Raptor Spatial genetic structure Circus aeruginosus Circus cyaneus Circus pygargus Microsatellites Relatedness [SDE]Environmental Sciences |
description |
International audience The Montagu’s harrier (Circus pygargus) is a semi-colonial raptor species widely but patchily distributed across the Palearctic region with recorded cases of philopatry and presence of extra-pair copulation. In order to assess Montagu’s harrier spatial genetic structure and contemporary gene flow, we developed 16 new microsatellite markers using 454 pyrosequencing. Genotypes of 117 chicks sampled in a 200 × 300 km farmland area in Central Western France were analyzed to characterize genetic polymorphism at each locus and regional and fine-scale genetic structure. Fourteen markers were found polymorphic, with a number of alleles ranging from 3 to 11. The expected and observed heterozygosities ranged from 0.36 to 0.856 and from 0.35 to 0.868, respectively. A single genetic unit was found at the regional scale with higher genetic similarity observed at a small spatial scale (up to 10 km). Our results are consistent with overall large-scale juvenile and adult dispersal together with small-scale male philopatry. Cross-species amplification of this set of microsatellites makers has been successful in two closely related harrier species: the marsh harrier (Circus aeruginosus) and the Hen harrier (Circus cyaneus) for which 14 and 12 markers were polymorphic, respectively. These new microsatellite markers could be used to study the population genetic structure, contemporary gene flow and parentage analyses in these three species and to conduct microsatellite-based demographic inferences on the Montagu’s harrier. |
author2 |
Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) LTSER «Zone Atelier Plaine & Val de Sevre» France Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de La Rochelle (ULR) Département de Biologie UFR Sciences - Université d'Angers France Université d'Angers (UA) |
format |
Article in Journal/Newspaper |
author |
Ribout, C. Villers, A. Ruault, S. Bretagnolle, V. Picard, D. Monceau, K. Gauffre, Bertrand |
author_facet |
Ribout, C. Villers, A. Ruault, S. Bretagnolle, V. Picard, D. Monceau, K. Gauffre, Bertrand |
author_sort |
Ribout, C. |
title |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_short |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_full |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_fullStr |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_full_unstemmed |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_sort |
fine-scale genetic structure in a high dispersal capacity raptor, the montagu’s harrier (circus pygargus), revealed by a set of novel microsatellite loci |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.archives-ouvertes.fr/hal-02108814 https://doi.org/10.1007/s10709-019-00053-7 |
long_lat |
ENVELOPE(-64.914,-64.914,61.317,61.317) |
geographic |
Hen |
geographic_facet |
Hen |
genre |
Circus cyaneus |
genre_facet |
Circus cyaneus |
op_source |
ISSN: 0016-6707 EISSN: 1573-6857 Genetica https://hal.archives-ouvertes.fr/hal-02108814 Genetica, Springer Verlag, 2019, 147 (1), pp.69-78. ⟨10.1007/s10709-019-00053-7⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10709-019-00053-7 info:eu-repo/semantics/altIdentifier/pmid/30671744 hal-02108814 https://hal.archives-ouvertes.fr/hal-02108814 doi:10.1007/s10709-019-00053-7 PRODINRA: 458309 WOS: 000460839000008 PUBMED: 30671744 |
op_doi |
https://doi.org/10.1007/s10709-019-00053-7 |
container_title |
Genetica |
container_volume |
147 |
container_issue |
1 |
container_start_page |
69 |
op_container_end_page |
78 |
_version_ |
1766391040710803456 |