New insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the Gfra1/Gdnf and Kit/Kitlg regulatory pathways in rainbow trout testis
Electronic supplementary material : the online version of this article (https://doi.org/10.1007/s10695-018-0547-4) contains supplementary material, which is available to authorized users. International audience The present study aimed to investigate whether the Gfra1/Gdnf and/or Kit/Kitlg regulatory...
Published in: | Fish Physiology and Biochemistry |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2018
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Online Access: | https://hal-univ-rennes1.archives-ouvertes.fr/hal-02019264 https://doi.org/10.1007/s10695-018-0547-4 |
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ftccsdartic:oai:HAL:hal-02019264v1 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
Stem cell factor Kit ligand Kit Spermatogenesis Oncorhynchus mykiss Germ stem cell Gfra1 Gdnf [SDV]Life Sciences [q-bio] |
spellingShingle |
Stem cell factor Kit ligand Kit Spermatogenesis Oncorhynchus mykiss Germ stem cell Gfra1 Gdnf [SDV]Life Sciences [q-bio] Maouche, Ahmed Curran, Edouard Goupil, Anne-Sophie Sambroni, Elisabeth Bellaïche, Johanna Le Gac, Florence Lareyre, Jean-Jacques New insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the Gfra1/Gdnf and Kit/Kitlg regulatory pathways in rainbow trout testis |
topic_facet |
Stem cell factor Kit ligand Kit Spermatogenesis Oncorhynchus mykiss Germ stem cell Gfra1 Gdnf [SDV]Life Sciences [q-bio] |
description |
Electronic supplementary material : the online version of this article (https://doi.org/10.1007/s10695-018-0547-4) contains supplementary material, which is available to authorized users. International audience The present study aimed to investigate whether the Gfra1/Gdnf and/or Kit/Kitlg regulatory pathways could be involved in the regulation of spermatogonial cell proliferation and/or differentiation in fish. Homologs of the mammalian gfra1, gdnf, kitr, and kitlg genes were identified in gnathostomes and reliable orthologous relationships were established using phylogenetic reconstructions and analyses of syntenic chromosomal fragments. Gene duplications and losses occurred specifically in teleost fish and members of the Salmoninae family including rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). Some duplicated genes exhibited distinct spatiotemporal expression profiles and were differently regulated by hormones in rainbow trout. Undifferentiated type A spermatogonia expressed higher levels of kitrb and kitra2 making them possible target cells for the gonadal kitlgb and somatic kitlga before the onset of spermatogenesis. Interestingly, gdnfa and gdnfb ohnologous genes were poorly expressed before the onset of spermatogenesis. The expression level of gdnfb was correlated with that of the vasa gene suggesting that the late increased abundance of gdnfb during spermatogenesis onset was due to the increased number of spermatogonial cells. gfra1a2 was expressed in undifferentiated typeA spermatogoniawhereas gfra1a1 was mainly detected in somatic cells. These observations indicate that the germinal gdnfb ligand could exert autocrine and paracrine functions on spermatogonia and on testicular somatic cells, respectively. Fsh and androgens inhibited gfra1a2 and gdnfb whereas gfra1a1 was stimulated by Fsh, androgens, and 17α, 20β progesterone. Finally, our data provide evidences that the molecular identity of the male germ stem cells changes during ontogenesis prior to spermatogenesis onset. |
author2 |
Laboratoire de Physiologie et Génomique des Poissons (LPGP) Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) Région Bretagne and the INRA PHASE department Martin Pšenicka ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015) |
format |
Article in Journal/Newspaper |
author |
Maouche, Ahmed Curran, Edouard Goupil, Anne-Sophie Sambroni, Elisabeth Bellaïche, Johanna Le Gac, Florence Lareyre, Jean-Jacques |
author_facet |
Maouche, Ahmed Curran, Edouard Goupil, Anne-Sophie Sambroni, Elisabeth Bellaïche, Johanna Le Gac, Florence Lareyre, Jean-Jacques |
author_sort |
Maouche, Ahmed |
title |
New insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the Gfra1/Gdnf and Kit/Kitlg regulatory pathways in rainbow trout testis |
title_short |
New insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the Gfra1/Gdnf and Kit/Kitlg regulatory pathways in rainbow trout testis |
title_full |
New insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the Gfra1/Gdnf and Kit/Kitlg regulatory pathways in rainbow trout testis |
title_fullStr |
New insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the Gfra1/Gdnf and Kit/Kitlg regulatory pathways in rainbow trout testis |
title_full_unstemmed |
New insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the Gfra1/Gdnf and Kit/Kitlg regulatory pathways in rainbow trout testis |
title_sort |
new insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the gfra1/gdnf and kit/kitlg regulatory pathways in rainbow trout testis |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal-univ-rennes1.archives-ouvertes.fr/hal-02019264 https://doi.org/10.1007/s10695-018-0547-4 |
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ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
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Vasa |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 0920-1742 EISSN: 1573-5168 Fish Physiology and Biochemistry https://hal-univ-rennes1.archives-ouvertes.fr/hal-02019264 Fish Physiology and Biochemistry, Springer Verlag, 2018, 44 (6), pp.1599-1616. ⟨10.1007/s10695-018-0547-4⟩ https://link.springer.com/journal/10695 |
op_relation |
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op_doi |
https://doi.org/10.1007/s10695-018-0547-4 |
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Fish Physiology and Biochemistry |
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44 |
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6 |
container_start_page |
1599 |
op_container_end_page |
1616 |
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spelling |
ftccsdartic:oai:HAL:hal-02019264v1 2023-05-15T15:32:55+02:00 New insights into the evolution, hormonal regulation, and spatiotemporal expression profiles of genes involved in the Gfra1/Gdnf and Kit/Kitlg regulatory pathways in rainbow trout testis Maouche, Ahmed Curran, Edouard Goupil, Anne-Sophie Sambroni, Elisabeth Bellaïche, Johanna Le Gac, Florence Lareyre, Jean-Jacques Laboratoire de Physiologie et Génomique des Poissons (LPGP) Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) Région Bretagne and the INRA PHASE department Martin Pšenicka ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015) 2018 https://hal-univ-rennes1.archives-ouvertes.fr/hal-02019264 https://doi.org/10.1007/s10695-018-0547-4 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10695-018-0547-4 info:eu-repo/semantics/altIdentifier/pmid/30121735 info:eu-repo/grantAgreement//652831/EU/AQUAculture infrastructures for EXCELlence in European fish research towards 2020/AQUAEXCEL2020 hal-02019264 https://hal-univ-rennes1.archives-ouvertes.fr/hal-02019264 doi:10.1007/s10695-018-0547-4 PRODINRA: 442753 PUBMED: 30121735 WOS: 000453883600016 ISSN: 0920-1742 EISSN: 1573-5168 Fish Physiology and Biochemistry https://hal-univ-rennes1.archives-ouvertes.fr/hal-02019264 Fish Physiology and Biochemistry, Springer Verlag, 2018, 44 (6), pp.1599-1616. ⟨10.1007/s10695-018-0547-4⟩ https://link.springer.com/journal/10695 Stem cell factor Kit ligand Kit Spermatogenesis Oncorhynchus mykiss Germ stem cell Gfra1 Gdnf [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1007/s10695-018-0547-4 2021-12-12T02:50:55Z Electronic supplementary material : the online version of this article (https://doi.org/10.1007/s10695-018-0547-4) contains supplementary material, which is available to authorized users. International audience The present study aimed to investigate whether the Gfra1/Gdnf and/or Kit/Kitlg regulatory pathways could be involved in the regulation of spermatogonial cell proliferation and/or differentiation in fish. Homologs of the mammalian gfra1, gdnf, kitr, and kitlg genes were identified in gnathostomes and reliable orthologous relationships were established using phylogenetic reconstructions and analyses of syntenic chromosomal fragments. Gene duplications and losses occurred specifically in teleost fish and members of the Salmoninae family including rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). Some duplicated genes exhibited distinct spatiotemporal expression profiles and were differently regulated by hormones in rainbow trout. Undifferentiated type A spermatogonia expressed higher levels of kitrb and kitra2 making them possible target cells for the gonadal kitlgb and somatic kitlga before the onset of spermatogenesis. Interestingly, gdnfa and gdnfb ohnologous genes were poorly expressed before the onset of spermatogenesis. The expression level of gdnfb was correlated with that of the vasa gene suggesting that the late increased abundance of gdnfb during spermatogenesis onset was due to the increased number of spermatogonial cells. gfra1a2 was expressed in undifferentiated typeA spermatogoniawhereas gfra1a1 was mainly detected in somatic cells. These observations indicate that the germinal gdnfb ligand could exert autocrine and paracrine functions on spermatogonia and on testicular somatic cells, respectively. Fsh and androgens inhibited gfra1a2 and gdnfb whereas gfra1a1 was stimulated by Fsh, androgens, and 17α, 20β progesterone. Finally, our data provide evidences that the molecular identity of the male germ stem cells changes during ontogenesis prior to spermatogenesis onset. Article in Journal/Newspaper Atlantic salmon Salmo salar Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Vasa ENVELOPE(25.177,25.177,67.587,67.587) Fish Physiology and Biochemistry 44 6 1599 1616 |