Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae
International audience Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs that causes bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using la...
Published in: | Microbiology |
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Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2015
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Online Access: | https://hal.archives-ouvertes.fr/hal-01967765 https://doi.org/10.1099/mic.0.000058 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
EXTRACELLULAR METALLOPROTEASE ACL ESCHERICHIA-COLI 1ST DESCRIPTION FATTY-ACIDS PURIFICATION PATHOGEN CORALLIILYTICUS CRASSOSTREA-GIGAS PACIFIC OYSTER BACILLARY NECROSIS [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
EXTRACELLULAR METALLOPROTEASE ACL ESCHERICHIA-COLI 1ST DESCRIPTION FATTY-ACIDS PURIFICATION PATHOGEN CORALLIILYTICUS CRASSOSTREA-GIGAS PACIFIC OYSTER BACILLARY NECROSIS [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Mersni-Achour, Rachida Cheikh, Yosra Ben Pichereau, Vianney Doghri, Ibtissem Etien, Cédric Degrémont, Lionel, Saulnier, Denis Fruitier-Arnaudin, Ingrid Travers, Marie-Agnès Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae |
topic_facet |
EXTRACELLULAR METALLOPROTEASE ACL ESCHERICHIA-COLI 1ST DESCRIPTION FATTY-ACIDS PURIFICATION PATHOGEN CORALLIILYTICUS CRASSOSTREA-GIGAS PACIFIC OYSTER BACILLARY NECROSIS [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs that causes bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with an LD50 of about 2.3x10(3) c.f.u. ml(-1) after 24 h. Moreover, complete or gel permeation HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECP fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by the expression level of some potential virulence factors. The overall results suggest that the pathology caused by the French V. tubiashii in C. gigas oysters is caused by a group of toxic factors and not only the metalloprotease. |
author2 |
LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie des Mollusques Marins, 17390 La Tremblade, France. (LGPMM) Santé, Génétique et Microbiologie des Mollusques (IFREMER SG2M) Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Fédération de Recherche en Environnement pour le Développement Durable - FR 3097 (FREDD) Université de La Rochelle (ULR) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Mersni-Achour, Rachida Cheikh, Yosra Ben Pichereau, Vianney Doghri, Ibtissem Etien, Cédric Degrémont, Lionel, Saulnier, Denis Fruitier-Arnaudin, Ingrid Travers, Marie-Agnès |
author_facet |
Mersni-Achour, Rachida Cheikh, Yosra Ben Pichereau, Vianney Doghri, Ibtissem Etien, Cédric Degrémont, Lionel, Saulnier, Denis Fruitier-Arnaudin, Ingrid Travers, Marie-Agnès |
author_sort |
Mersni-Achour, Rachida |
title |
Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae |
title_short |
Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae |
title_full |
Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae |
title_fullStr |
Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae |
title_full_unstemmed |
Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae |
title_sort |
factors other than metalloprotease are required for full virulence of french vibrio tubiashii isolates in oyster larvae |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.archives-ouvertes.fr/hal-01967765 https://doi.org/10.1099/mic.0.000058 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1350-0872 EISSN: 1465-2080 Microbiology https://hal.archives-ouvertes.fr/hal-01967765 Microbiology, Microbiology Society, 2015, 161 (5), pp.997-1007. ⟨10.1099/mic.0.000058⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000058 hal-01967765 https://hal.archives-ouvertes.fr/hal-01967765 doi:10.1099/mic.0.000058 |
op_doi |
https://doi.org/10.1099/mic.0.000058 |
container_title |
Microbiology |
container_volume |
161 |
container_issue |
5 |
container_start_page |
997 |
op_container_end_page |
1007 |
_version_ |
1766393696698236928 |
spelling |
ftccsdartic:oai:HAL:hal-01967765v1 2023-05-15T15:57:59+02:00 Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae Mersni-Achour, Rachida Cheikh, Yosra Ben Pichereau, Vianney Doghri, Ibtissem Etien, Cédric Degrémont, Lionel, Saulnier, Denis Fruitier-Arnaudin, Ingrid Travers, Marie-Agnès LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie des Mollusques Marins, 17390 La Tremblade, France. (LGPMM) Santé, Génétique et Microbiologie des Mollusques (IFREMER SG2M) Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Fédération de Recherche en Environnement pour le Développement Durable - FR 3097 (FREDD) Université de La Rochelle (ULR) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2015-05 https://hal.archives-ouvertes.fr/hal-01967765 https://doi.org/10.1099/mic.0.000058 en eng HAL CCSD Microbiology Society info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000058 hal-01967765 https://hal.archives-ouvertes.fr/hal-01967765 doi:10.1099/mic.0.000058 ISSN: 1350-0872 EISSN: 1465-2080 Microbiology https://hal.archives-ouvertes.fr/hal-01967765 Microbiology, Microbiology Society, 2015, 161 (5), pp.997-1007. ⟨10.1099/mic.0.000058⟩ EXTRACELLULAR METALLOPROTEASE ACL ESCHERICHIA-COLI 1ST DESCRIPTION FATTY-ACIDS PURIFICATION PATHOGEN CORALLIILYTICUS CRASSOSTREA-GIGAS PACIFIC OYSTER BACILLARY NECROSIS [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2015 ftccsdartic https://doi.org/10.1099/mic.0.000058 2021-12-19T02:07:09Z International audience Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs that causes bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with an LD50 of about 2.3x10(3) c.f.u. ml(-1) after 24 h. Moreover, complete or gel permeation HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECP fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by the expression level of some potential virulence factors. The overall results suggest that the pathology caused by the French V. tubiashii in C. gigas oysters is caused by a group of toxic factors and not only the metalloprotease. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Microbiology 161 5 997 1007 |