Comparison of European eel sperm cryopreservation protocols with standardization as a target
The critical situation of the European eel (Anguilla anguilla) has urged the development of sperm cryopre-servation protocols for reproduction in captivity and cryobanking. In the last years, two research groups havedeveloped their own protocols in Spain and Hungary with positive results, but difficul...
Published in: | Aquaculture |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2019
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Online Access: | https://hal.archives-ouvertes.fr/hal-01892956 https://hal.archives-ouvertes.fr/hal-01892956/document https://hal.archives-ouvertes.fr/hal-01892956/file/Jusdado2018.pdf https://doi.org/10.1016/j.aquaculture.2018.09.006 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
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language |
English |
topic |
anguilla anguilla methanol DMSO DNA methylation epigenetics [SDV]Life Sciences [q-bio] |
spellingShingle |
anguilla anguilla methanol DMSO DNA methylation epigenetics [SDV]Life Sciences [q-bio] Herranz-Jusdado, J.G. Gallego, V. Morini, M. Rozenfeld, C. Pérez, L. Kása, E. Kollár, T. Depince, Alexandra Labbé, Catherine Asturiano, J.F. Comparison of European eel sperm cryopreservation protocols with standardization as a target |
topic_facet |
anguilla anguilla methanol DMSO DNA methylation epigenetics [SDV]Life Sciences [q-bio] |
description |
The critical situation of the European eel (Anguilla anguilla) has urged the development of sperm cryopre-servation protocols for reproduction in captivity and cryobanking. In the last years, two research groups havedeveloped their own protocols in Spain and Hungary with positive results, but difficult to compare.Here, a series of experiments were conducted to test the quality of thawed sperm after using both protocols,determining which of them produce the best results and aiming for standardization. The quality of thawed spermwas assessed by studying the motility and kinetic values of thawed sperm from both cryopreservation protocolsusing a computer-assisted sperm analysis (CASA-Mot) system. In addition, a viability analysis was performedusing flow cytometry to test if the cryoprotectants or the freezing-thawing process led to a reduction in sper-matozoa survival. Furthermore, since during cryopreservation the sperm was treated with methylated cryo-protectants (DMSO or methanol) that may induce epigenetic changes in the sperm DNA (cytosine methylation)and could affect the offspring, we conducted a luminometric methylation assay (LUMA) to study the DNAmethylation levels induced by both protocols.In this work, all the above-mentioned parameters were analyzed in fresh and frozen-thawed sperm samples.Our results showed that thawed sperm samples from both protocols presented lower sperm motility and velocity,and lower percentage of live cells than those shown in fresh sperm samples. Furthermore, sperm samples fromthe methanol based protocol showed significantly higher motility, velocity and percentage of live spermatozoathan the same sperm samples treated with the DMSO based protocol. In addition, the DMSO based protocolinduced a hypomethylation of sperm DNA compared to fresh samples whereas the methanol based protocol didnot alter sperm DNA methylation level. Our results indicate that the methanol based protocol is a more suitableprotocol that preserves better the motility and genetic qualities of the European eel sperm. |
author2 |
Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal Universitat Politècnica de València (UPV) Department of Aquaculture Swedish University of Agricultural Sciences (SLU) Laboratoire de Physiologie et Génomique des Poissons (LPGP) Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) Funded by the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement n° 642893 (IMPRESS), including the JGHJ and CR predoctoral contracts, the French CRB Anim project «Investissements d'avenir», ANR-11-INBS-0003, as well as the Hungarian EFOP 3.6.3.-VEKOP 16.-2017-00008 project, and by the Higher Education Institutional Excellence Program (1783-3/2018/FEKUTSRAT) awarded by the Ministry of Human Capacities within the framework of water related researches of Szent István University. MM, EK, TK and ÁH were granted with Short Term Scientific Missions by the COST Office (COST Action FA1205:AQUAGAMETE) ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) European Project: 642893,H2020,H2020-MSCA-ITN-2014,IMPRESS(2015) |
format |
Article in Journal/Newspaper |
author |
Herranz-Jusdado, J.G. Gallego, V. Morini, M. Rozenfeld, C. Pérez, L. Kása, E. Kollár, T. Depince, Alexandra Labbé, Catherine Asturiano, J.F. |
author_facet |
Herranz-Jusdado, J.G. Gallego, V. Morini, M. Rozenfeld, C. Pérez, L. Kása, E. Kollár, T. Depince, Alexandra Labbé, Catherine Asturiano, J.F. |
author_sort |
Herranz-Jusdado, J.G. |
title |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
title_short |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
title_full |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
title_fullStr |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
title_full_unstemmed |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
title_sort |
comparison of european eel sperm cryopreservation protocols with standardization as a target |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.archives-ouvertes.fr/hal-01892956 https://hal.archives-ouvertes.fr/hal-01892956/document https://hal.archives-ouvertes.fr/hal-01892956/file/Jusdado2018.pdf https://doi.org/10.1016/j.aquaculture.2018.09.006 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 0044-8486 Aquaculture https://hal.archives-ouvertes.fr/hal-01892956 Aquaculture, Elsevier, 2019, 498, pp.539-544. ⟨10.1016/j.aquaculture.2018.09.006⟩ https://www.journals.elsevier.com/aquaculture |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2018.09.006 info:eu-repo/grantAgreement//642893/EU/Improved production strategies for endangered freshwater species./IMPRESS hal-01892956 https://hal.archives-ouvertes.fr/hal-01892956 https://hal.archives-ouvertes.fr/hal-01892956/document https://hal.archives-ouvertes.fr/hal-01892956/file/Jusdado2018.pdf PRODINRA: 448905 doi:10.1016/j.aquaculture.2018.09.006 WOS: 000445802900064 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2018.09.006 |
container_title |
Aquaculture |
container_volume |
498 |
container_start_page |
539 |
op_container_end_page |
544 |
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spelling |
ftccsdartic:oai:HAL:hal-01892956v1 2023-05-15T13:27:31+02:00 Comparison of European eel sperm cryopreservation protocols with standardization as a target Herranz-Jusdado, J.G. Gallego, V. Morini, M. Rozenfeld, C. Pérez, L. Kása, E. Kollár, T. Depince, Alexandra Labbé, Catherine Asturiano, J.F. Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal Universitat Politècnica de València (UPV) Department of Aquaculture Swedish University of Agricultural Sciences (SLU) Laboratoire de Physiologie et Génomique des Poissons (LPGP) Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) Funded by the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement n° 642893 (IMPRESS), including the JGHJ and CR predoctoral contracts, the French CRB Anim project «Investissements d'avenir», ANR-11-INBS-0003, as well as the Hungarian EFOP 3.6.3.-VEKOP 16.-2017-00008 project, and by the Higher Education Institutional Excellence Program (1783-3/2018/FEKUTSRAT) awarded by the Ministry of Human Capacities within the framework of water related researches of Szent István University. MM, EK, TK and ÁH were granted with Short Term Scientific Missions by the COST Office (COST Action FA1205:AQUAGAMETE) ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) European Project: 642893,H2020,H2020-MSCA-ITN-2014,IMPRESS(2015) 2019 https://hal.archives-ouvertes.fr/hal-01892956 https://hal.archives-ouvertes.fr/hal-01892956/document https://hal.archives-ouvertes.fr/hal-01892956/file/Jusdado2018.pdf https://doi.org/10.1016/j.aquaculture.2018.09.006 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2018.09.006 info:eu-repo/grantAgreement//642893/EU/Improved production strategies for endangered freshwater species./IMPRESS hal-01892956 https://hal.archives-ouvertes.fr/hal-01892956 https://hal.archives-ouvertes.fr/hal-01892956/document https://hal.archives-ouvertes.fr/hal-01892956/file/Jusdado2018.pdf PRODINRA: 448905 doi:10.1016/j.aquaculture.2018.09.006 WOS: 000445802900064 info:eu-repo/semantics/OpenAccess ISSN: 0044-8486 Aquaculture https://hal.archives-ouvertes.fr/hal-01892956 Aquaculture, Elsevier, 2019, 498, pp.539-544. ⟨10.1016/j.aquaculture.2018.09.006⟩ https://www.journals.elsevier.com/aquaculture anguilla anguilla methanol DMSO DNA methylation epigenetics [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2019 ftccsdartic https://doi.org/10.1016/j.aquaculture.2018.09.006 2021-12-12T03:01:58Z The critical situation of the European eel (Anguilla anguilla) has urged the development of sperm cryopre-servation protocols for reproduction in captivity and cryobanking. In the last years, two research groups havedeveloped their own protocols in Spain and Hungary with positive results, but difficult to compare.Here, a series of experiments were conducted to test the quality of thawed sperm after using both protocols,determining which of them produce the best results and aiming for standardization. The quality of thawed spermwas assessed by studying the motility and kinetic values of thawed sperm from both cryopreservation protocolsusing a computer-assisted sperm analysis (CASA-Mot) system. In addition, a viability analysis was performedusing flow cytometry to test if the cryoprotectants or the freezing-thawing process led to a reduction in sper-matozoa survival. Furthermore, since during cryopreservation the sperm was treated with methylated cryo-protectants (DMSO or methanol) that may induce epigenetic changes in the sperm DNA (cytosine methylation)and could affect the offspring, we conducted a luminometric methylation assay (LUMA) to study the DNAmethylation levels induced by both protocols.In this work, all the above-mentioned parameters were analyzed in fresh and frozen-thawed sperm samples.Our results showed that thawed sperm samples from both protocols presented lower sperm motility and velocity,and lower percentage of live cells than those shown in fresh sperm samples. Furthermore, sperm samples fromthe methanol based protocol showed significantly higher motility, velocity and percentage of live spermatozoathan the same sperm samples treated with the DMSO based protocol. In addition, the DMSO based protocolinduced a hypomethylation of sperm DNA compared to fresh samples whereas the methanol based protocol didnot alter sperm DNA methylation level. Our results indicate that the methanol based protocol is a more suitableprotocol that preserves better the motility and genetic qualities of the European eel sperm. Article in Journal/Newspaper Anguilla anguilla Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Aquaculture 498 539 544 |