Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas
International audience Although tachykinin-like neuropeptides have been identified in molluscs more than two decades ago, knowledge on their function and signalling has so far remained largely elusive. We developed a cell-based assay to address the functionality of the tachykinin G-protein coupled r...
Published in: | General and Comparative Endocrinology |
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Online Access: | https://hal.sorbonne-universite.fr/hal-01838268 https://hal.sorbonne-universite.fr/hal-01838268/document https://hal.sorbonne-universite.fr/hal-01838268/file/Oyster%20TK%20signalling%20HAL.pdf https://doi.org/10.1016/j.ygcen.2018.05.003 |
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ftccsdartic:oai:HAL:hal-01838268v1 2023-05-15T15:57:52+02:00 Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas Dubos, Marie-Pierre Zels, Sven Schwartz, Julie Pasquier, Jeremy Schoofs, Liliane Favrel, Pascal Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Functional Genomics and Proteomics Group 2018-05 https://hal.sorbonne-universite.fr/hal-01838268 https://hal.sorbonne-universite.fr/hal-01838268/document https://hal.sorbonne-universite.fr/hal-01838268/file/Oyster%20TK%20signalling%20HAL.pdf https://doi.org/10.1016/j.ygcen.2018.05.003 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2018.05.003 hal-01838268 https://hal.sorbonne-universite.fr/hal-01838268 https://hal.sorbonne-universite.fr/hal-01838268/document https://hal.sorbonne-universite.fr/hal-01838268/file/Oyster%20TK%20signalling%20HAL.pdf doi:10.1016/j.ygcen.2018.05.003 info:eu-repo/semantics/OpenAccess ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.sorbonne-universite.fr/hal-01838268 General and Comparative Endocrinology, Elsevier, 2018, ⟨10.1016/j.ygcen.2018.05.003⟩ Mollusc neuropeptide Tachykinin signalling feeding [SDV.BID]Life Sciences [q-bio]/Biodiversity info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1016/j.ygcen.2018.05.003 2021-12-05T02:36:02Z International audience Although tachykinin-like neuropeptides have been identified in molluscs more than two decades ago, knowledge on their function and signalling has so far remained largely elusive. We developed a cell-based assay to address the functionality of the tachykinin G-protein coupled receptor (Cragi-TKR) in the oyster Crassostrea gigas. The oyster tachykinin neuropeptides that are derived from the tachykinin precursor gene Cragi-TK activate the Cragi-TKR in nanomolar concentrations. Receptor activation is sensitive to Ala-substitution of critical Cragi-TK amino acid residues. The Cragi-TKR gene is expressed in a variety of tissues, albeit at higher levels in the visceral ganglia (VG) of the nervous system. Fluctuations of Cragi-TKR expression is in line with a role for TK signalling in C. gigas reproduction. The expression level of the Cragi-TK gene in the VG depends on the nutritional status of the oyster, suggesting a role for TK signalling in the complex regulation of feeding in C. gigas. Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) General and Comparative Endocrinology 266 110 118 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Mollusc neuropeptide Tachykinin signalling feeding [SDV.BID]Life Sciences [q-bio]/Biodiversity |
spellingShingle |
Mollusc neuropeptide Tachykinin signalling feeding [SDV.BID]Life Sciences [q-bio]/Biodiversity Dubos, Marie-Pierre Zels, Sven Schwartz, Julie Pasquier, Jeremy Schoofs, Liliane Favrel, Pascal Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas |
topic_facet |
Mollusc neuropeptide Tachykinin signalling feeding [SDV.BID]Life Sciences [q-bio]/Biodiversity |
description |
International audience Although tachykinin-like neuropeptides have been identified in molluscs more than two decades ago, knowledge on their function and signalling has so far remained largely elusive. We developed a cell-based assay to address the functionality of the tachykinin G-protein coupled receptor (Cragi-TKR) in the oyster Crassostrea gigas. The oyster tachykinin neuropeptides that are derived from the tachykinin precursor gene Cragi-TK activate the Cragi-TKR in nanomolar concentrations. Receptor activation is sensitive to Ala-substitution of critical Cragi-TK amino acid residues. The Cragi-TKR gene is expressed in a variety of tissues, albeit at higher levels in the visceral ganglia (VG) of the nervous system. Fluctuations of Cragi-TKR expression is in line with a role for TK signalling in C. gigas reproduction. The expression level of the Cragi-TK gene in the VG depends on the nutritional status of the oyster, suggesting a role for TK signalling in the complex regulation of feeding in C. gigas. |
author2 |
Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Functional Genomics and Proteomics Group |
format |
Article in Journal/Newspaper |
author |
Dubos, Marie-Pierre Zels, Sven Schwartz, Julie Pasquier, Jeremy Schoofs, Liliane Favrel, Pascal |
author_facet |
Dubos, Marie-Pierre Zels, Sven Schwartz, Julie Pasquier, Jeremy Schoofs, Liliane Favrel, Pascal |
author_sort |
Dubos, Marie-Pierre |
title |
Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas |
title_short |
Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas |
title_full |
Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas |
title_fullStr |
Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas |
title_full_unstemmed |
Characterization of a tachykinin signalling system in the bivalve mollusc Crassostrea gigas |
title_sort |
characterization of a tachykinin signalling system in the bivalve mollusc crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.sorbonne-universite.fr/hal-01838268 https://hal.sorbonne-universite.fr/hal-01838268/document https://hal.sorbonne-universite.fr/hal-01838268/file/Oyster%20TK%20signalling%20HAL.pdf https://doi.org/10.1016/j.ygcen.2018.05.003 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.sorbonne-universite.fr/hal-01838268 General and Comparative Endocrinology, Elsevier, 2018, ⟨10.1016/j.ygcen.2018.05.003⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2018.05.003 hal-01838268 https://hal.sorbonne-universite.fr/hal-01838268 https://hal.sorbonne-universite.fr/hal-01838268/document https://hal.sorbonne-universite.fr/hal-01838268/file/Oyster%20TK%20signalling%20HAL.pdf doi:10.1016/j.ygcen.2018.05.003 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.ygcen.2018.05.003 |
container_title |
General and Comparative Endocrinology |
container_volume |
266 |
container_start_page |
110 |
op_container_end_page |
118 |
_version_ |
1766393571070443520 |