Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field
International audience Since 2008, juvenile Crassostrea gigas oysters have suffered from massive mortalities in European fanning areas. This disease of complex etiology is still incompletely understood. Triggered by an elevated seawater temperature, it has been associated to infections by a herpes v...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2018
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Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-01759229 https://hal.archives-ouvertes.fr/hal-01759229/document https://hal.archives-ouvertes.fr/hal-01759229/file/De-Lorgeril_etal_FSI_2018.pdf https://doi.org/10.1016/j.fsi.2018.03.027 |
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ftccsdartic:oai:HAL:hal-01759229v1 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Host pathogen interaction Innate immunity Invertebrate Mollusk In situ mortality Total bacteria Crassostrea gigas ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Host pathogen interaction Innate immunity Invertebrate Mollusk In situ mortality Total bacteria Crassostrea gigas ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology De Lorgeril, Julien Escoubas, Jean-Michel Loubiere, Vincent Pernet, Fabrice Le Gall, Patrik Vergnes, Agnes Aujoulat, Fabien Jeannot, Jean-Luc Jumas-Bilak, Estelle Got, Patrice Gueguen, Yannick Destoumieux-Garzón, Delphine Bachère, Evelyne Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field |
topic_facet |
Host pathogen interaction Innate immunity Invertebrate Mollusk In situ mortality Total bacteria Crassostrea gigas ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Since 2008, juvenile Crassostrea gigas oysters have suffered from massive mortalities in European fanning areas. This disease of complex etiology is still incompletely understood. Triggered by an elevated seawater temperature, it has been associated to infections by a herpes virus named OsHV-1 as well as pathogenic vibrios of the Splendidus Glade. Ruling out the complexity of the disease, most of our current knowledge has been acquired in controlled experiments. Among the many unsolved questions, it is still ignored what role immunity plays in the capacity oysters have to survive an infectious episode. Here we show that juvenile oysters susceptible to the disease mount an inefficient immune response associated with microbial permissiveness and death. We found that, in contrast to resistant adult oysters having survived an earlier episode of mortality, susceptible juvenile oysters never exposed to infectious episodes died by more than 90% in a field experiment. Susceptible oysters were heavily colonized by OsHV-1 herpes virus as well as bacteria including vibrios potentially pathogenic for oysters, which proliferated in oyster flesh and body fluids during the mortality event. Nonetheless, susceptible oysters were found to sense microbes as indicated by an overexpression of immune receptors and immune signaling pathways. However, they did not express important immune effectors involved in antimicrobial immunity and apoptosis and showed repressed expression of genes involved in ROS and metal homeostasis. This contrasted with resistant oysters, which expressed those important effectors, controlled bacterial and viral colonization and showed 100% survival to the mortality event. Altogether, our results demonstrate that the immune response mounted by susceptible oysters lacks some important immune functions and fails in controlling microbial proliferation. This study opens the way to more holistic studies on the "mass mortality syndrome", which are now required to decipher the sequence of ... |
author2 |
Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD) Hydrosciences Montpellier (HSM) Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes lagunaires : organisation biologique et fonctionnement (ECOLAG) Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
De Lorgeril, Julien Escoubas, Jean-Michel Loubiere, Vincent Pernet, Fabrice Le Gall, Patrik Vergnes, Agnes Aujoulat, Fabien Jeannot, Jean-Luc Jumas-Bilak, Estelle Got, Patrice Gueguen, Yannick Destoumieux-Garzón, Delphine Bachère, Evelyne |
author_facet |
De Lorgeril, Julien Escoubas, Jean-Michel Loubiere, Vincent Pernet, Fabrice Le Gall, Patrik Vergnes, Agnes Aujoulat, Fabien Jeannot, Jean-Luc Jumas-Bilak, Estelle Got, Patrice Gueguen, Yannick Destoumieux-Garzón, Delphine Bachère, Evelyne |
author_sort |
De Lorgeril, Julien |
title |
Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field |
title_short |
Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field |
title_full |
Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field |
title_fullStr |
Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field |
title_full_unstemmed |
Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field |
title_sort |
inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.archives-ouvertes.fr/hal-01759229 https://hal.archives-ouvertes.fr/hal-01759229/document https://hal.archives-ouvertes.fr/hal-01759229/file/De-Lorgeril_etal_FSI_2018.pdf https://doi.org/10.1016/j.fsi.2018.03.027 |
long_lat |
ENVELOPE(-60.632,-60.632,-72.404,-72.404) |
geographic |
Fanning |
geographic_facet |
Fanning |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.archives-ouvertes.fr/hal-01759229 Fish and Shellfish Immunology, Elsevier, 2018, 77, pp.156 - 163. ⟨10.1016/j.fsi.2018.03.027⟩ http://www.sciencedirect.com/science/article/pii/S105046481830144X |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2018.03.027 hal-01759229 https://hal.archives-ouvertes.fr/hal-01759229 https://hal.archives-ouvertes.fr/hal-01759229/document https://hal.archives-ouvertes.fr/hal-01759229/file/De-Lorgeril_etal_FSI_2018.pdf doi:10.1016/j.fsi.2018.03.027 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2018.03.027 |
container_title |
Fish & Shellfish Immunology |
container_volume |
77 |
container_start_page |
156 |
op_container_end_page |
163 |
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1766394299074740224 |
spelling |
ftccsdartic:oai:HAL:hal-01759229v1 2023-05-15T15:58:33+02:00 Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field De Lorgeril, Julien Escoubas, Jean-Michel Loubiere, Vincent Pernet, Fabrice Le Gall, Patrik Vergnes, Agnes Aujoulat, Fabien Jeannot, Jean-Luc Jumas-Bilak, Estelle Got, Patrice Gueguen, Yannick Destoumieux-Garzón, Delphine Bachère, Evelyne Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD) Hydrosciences Montpellier (HSM) Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes lagunaires : organisation biologique et fonctionnement (ECOLAG) Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2018-06 https://hal.archives-ouvertes.fr/hal-01759229 https://hal.archives-ouvertes.fr/hal-01759229/document https://hal.archives-ouvertes.fr/hal-01759229/file/De-Lorgeril_etal_FSI_2018.pdf https://doi.org/10.1016/j.fsi.2018.03.027 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2018.03.027 hal-01759229 https://hal.archives-ouvertes.fr/hal-01759229 https://hal.archives-ouvertes.fr/hal-01759229/document https://hal.archives-ouvertes.fr/hal-01759229/file/De-Lorgeril_etal_FSI_2018.pdf doi:10.1016/j.fsi.2018.03.027 info:eu-repo/semantics/OpenAccess ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.archives-ouvertes.fr/hal-01759229 Fish and Shellfish Immunology, Elsevier, 2018, 77, pp.156 - 163. ⟨10.1016/j.fsi.2018.03.027⟩ http://www.sciencedirect.com/science/article/pii/S105046481830144X Host pathogen interaction Innate immunity Invertebrate Mollusk In situ mortality Total bacteria Crassostrea gigas ACL [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1016/j.fsi.2018.03.027 2021-12-19T01:18:10Z International audience Since 2008, juvenile Crassostrea gigas oysters have suffered from massive mortalities in European fanning areas. This disease of complex etiology is still incompletely understood. Triggered by an elevated seawater temperature, it has been associated to infections by a herpes virus named OsHV-1 as well as pathogenic vibrios of the Splendidus Glade. Ruling out the complexity of the disease, most of our current knowledge has been acquired in controlled experiments. Among the many unsolved questions, it is still ignored what role immunity plays in the capacity oysters have to survive an infectious episode. Here we show that juvenile oysters susceptible to the disease mount an inefficient immune response associated with microbial permissiveness and death. We found that, in contrast to resistant adult oysters having survived an earlier episode of mortality, susceptible juvenile oysters never exposed to infectious episodes died by more than 90% in a field experiment. Susceptible oysters were heavily colonized by OsHV-1 herpes virus as well as bacteria including vibrios potentially pathogenic for oysters, which proliferated in oyster flesh and body fluids during the mortality event. Nonetheless, susceptible oysters were found to sense microbes as indicated by an overexpression of immune receptors and immune signaling pathways. However, they did not express important immune effectors involved in antimicrobial immunity and apoptosis and showed repressed expression of genes involved in ROS and metal homeostasis. This contrasted with resistant oysters, which expressed those important effectors, controlled bacterial and viral colonization and showed 100% survival to the mortality event. Altogether, our results demonstrate that the immune response mounted by susceptible oysters lacks some important immune functions and fails in controlling microbial proliferation. This study opens the way to more holistic studies on the "mass mortality syndrome", which are now required to decipher the sequence of ... Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Fanning ENVELOPE(-60.632,-60.632,-72.404,-72.404) Fish & Shellfish Immunology 77 156 163 |