HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress
International audience Background: Heat stress proteins are implicated in stabilizing and refolding denatured proteins in vertebrates and invertebrates. Members of the Hsp70 gene family comprise the cognate heat shock protein (Hsc70) and inducible heat shock protein (Hsp70). However, the cDNA sequen...
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ftccsdartic:oai:HAL:hal-01759214v1 2023-05-15T13:38:08+02:00 HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress Gonzalez-Aravena, Marcelo Calfio, Camila Mercado, Luis Morales-Lange, Byron Bethke, Jorn De Lorgeril, Julien Cárdenas, Cesar Instituto Antartico Chileno Pontificia Universidad Católica de Valparaíso (PUCV) Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) 2018 https://hal.archives-ouvertes.fr/hal-01759214 https://hal.archives-ouvertes.fr/hal-01759214/document https://hal.archives-ouvertes.fr/hal-01759214/file/Gonzales-BiolRes-HSP70.pdf https://doi.org/10.1186/s40659-018-0156-9 en eng HAL CCSD Sociedad de Biología de Chile info:eu-repo/semantics/altIdentifier/doi/10.1186/s40659-018-0156-9 hal-01759214 https://hal.archives-ouvertes.fr/hal-01759214 https://hal.archives-ouvertes.fr/hal-01759214/document https://hal.archives-ouvertes.fr/hal-01759214/file/Gonzales-BiolRes-HSP70.pdf doi:10.1186/s40659-018-0156-9 info:eu-repo/semantics/OpenAccess ISSN: 0716-9760 EISSN: 0717-6287 Biological Research https://hal.archives-ouvertes.fr/hal-01759214 Biological Research, Sociedad de Biología de Chile, 2018, 51, pp.8. ⟨10.1186/s40659-018-0156-9⟩ Hsp70 Coelomocytes Antarctica Echinoderms Climate change [SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/Bioclimatology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1186/s40659-018-0156-9 2021-11-07T03:05:32Z International audience Background: Heat stress proteins are implicated in stabilizing and refolding denatured proteins in vertebrates and invertebrates. Members of the Hsp70 gene family comprise the cognate heat shock protein (Hsc70) and inducible heat shock protein (Hsp70). However, the cDNA sequence and the expression of Hsp70 in the Antarctic sea urchin are unknown.Methods: We amplified and cloned a transcript sequence of 1991 bp from the Antarctic sea urchin Sterechinus neumayeri, experimentally exposed to heat stress (5 and 10 °C for 1, 24 and 48 h). RACE-PCR and qPCR were employed to determine Hsp70 gene expression, while western blot and ELISA methods were used to determine protein expression.Results: The sequence obtained from S. neumayeri showed high identity with Hsp70 members. Several Hsp70 family features were identified in the deduced amino acid sequence and they indicate that the isolated Hsp70 is related to the cognate heat shock protein type. The corresponding 70 kDa protein, called Sn-Hsp70, was immune detected in the coelomocytes and the digestive tract of S. neumayeri using a monospecific polyclonal antibody. We showed that S. neumayeri do not respond to acute heat stress by up-regulation of Sn-Hsp70 at transcript and protein level. Furthermore, the Sn-Hsp70 protein expression was not induced in the digestive tract.Conclusions:Our results provide the first molecular evidence that Sn-Hsp70 is expressed constitutively and is non-induced by heat stress in S. neumayeri. Article in Journal/Newspaper Antarc* Antarctic Antarctica Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Antarctic The Antarctic Biological Research 51 1 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Hsp70 Coelomocytes Antarctica Echinoderms Climate change [SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/Bioclimatology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] |
spellingShingle |
Hsp70 Coelomocytes Antarctica Echinoderms Climate change [SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/Bioclimatology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Gonzalez-Aravena, Marcelo Calfio, Camila Mercado, Luis Morales-Lange, Byron Bethke, Jorn De Lorgeril, Julien Cárdenas, Cesar HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress |
topic_facet |
Hsp70 Coelomocytes Antarctica Echinoderms Climate change [SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/Bioclimatology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] |
description |
International audience Background: Heat stress proteins are implicated in stabilizing and refolding denatured proteins in vertebrates and invertebrates. Members of the Hsp70 gene family comprise the cognate heat shock protein (Hsc70) and inducible heat shock protein (Hsp70). However, the cDNA sequence and the expression of Hsp70 in the Antarctic sea urchin are unknown.Methods: We amplified and cloned a transcript sequence of 1991 bp from the Antarctic sea urchin Sterechinus neumayeri, experimentally exposed to heat stress (5 and 10 °C for 1, 24 and 48 h). RACE-PCR and qPCR were employed to determine Hsp70 gene expression, while western blot and ELISA methods were used to determine protein expression.Results: The sequence obtained from S. neumayeri showed high identity with Hsp70 members. Several Hsp70 family features were identified in the deduced amino acid sequence and they indicate that the isolated Hsp70 is related to the cognate heat shock protein type. The corresponding 70 kDa protein, called Sn-Hsp70, was immune detected in the coelomocytes and the digestive tract of S. neumayeri using a monospecific polyclonal antibody. We showed that S. neumayeri do not respond to acute heat stress by up-regulation of Sn-Hsp70 at transcript and protein level. Furthermore, the Sn-Hsp70 protein expression was not induced in the digestive tract.Conclusions:Our results provide the first molecular evidence that Sn-Hsp70 is expressed constitutively and is non-induced by heat stress in S. neumayeri. |
author2 |
Instituto Antartico Chileno Pontificia Universidad Católica de Valparaíso (PUCV) Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) |
format |
Article in Journal/Newspaper |
author |
Gonzalez-Aravena, Marcelo Calfio, Camila Mercado, Luis Morales-Lange, Byron Bethke, Jorn De Lorgeril, Julien Cárdenas, Cesar |
author_facet |
Gonzalez-Aravena, Marcelo Calfio, Camila Mercado, Luis Morales-Lange, Byron Bethke, Jorn De Lorgeril, Julien Cárdenas, Cesar |
author_sort |
Gonzalez-Aravena, Marcelo |
title |
HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress |
title_short |
HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress |
title_full |
HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress |
title_fullStr |
HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress |
title_full_unstemmed |
HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress |
title_sort |
hsp70 from the antarctic sea urchin sterechinus neumayeri: molecular characterization and expression in response to heat stress |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.archives-ouvertes.fr/hal-01759214 https://hal.archives-ouvertes.fr/hal-01759214/document https://hal.archives-ouvertes.fr/hal-01759214/file/Gonzales-BiolRes-HSP70.pdf https://doi.org/10.1186/s40659-018-0156-9 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
ISSN: 0716-9760 EISSN: 0717-6287 Biological Research https://hal.archives-ouvertes.fr/hal-01759214 Biological Research, Sociedad de Biología de Chile, 2018, 51, pp.8. ⟨10.1186/s40659-018-0156-9⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s40659-018-0156-9 hal-01759214 https://hal.archives-ouvertes.fr/hal-01759214 https://hal.archives-ouvertes.fr/hal-01759214/document https://hal.archives-ouvertes.fr/hal-01759214/file/Gonzales-BiolRes-HSP70.pdf doi:10.1186/s40659-018-0156-9 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s40659-018-0156-9 |
container_title |
Biological Research |
container_volume |
51 |
container_issue |
1 |
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1766101760207749120 |