Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides
International audience An in situ hybridization method was applied to the identification of marine cyanobacteria assignable to the genus Prochlorococcus using horseradish-peroxidase-labelled 16S rRNA-targeted oligonucleotide probes in combination with tyramide signal amplification (TSA). With this m...
Published in: | Microbiology |
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Main Authors: | , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2001
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Online Access: | https://hal.archives-ouvertes.fr/hal-01651552 https://doi.org/10.1099/00221287-147-7-1731 |
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ftccsdartic:oai:HAL:hal-01651552v1 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
16S rRNA TSA oligonucleotide probes Red Sea cyanobacteria MESH: Colony Count Microbial MESH: Cyanobacteria/classification MESH: DNA Ribosomal/analysis MESH: Ecology MESH: Flow Cytometry MESH: Genes rRNA MESH: Genotype MESH: In Situ Hybridization Fluorescence MESH: Molecular Sequence Data MESH: Oligonucleotide Probes/genetics MESH: Phylogeny MESH: RNA Ribosomal 16S/genetics MESH: Seawater/microbiology MESH: Sequence Analysis DNA MESH: Cyanobacteria/genetics MESH: Cyanobacteria/physiology [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
16S rRNA TSA oligonucleotide probes Red Sea cyanobacteria MESH: Colony Count Microbial MESH: Cyanobacteria/classification MESH: DNA Ribosomal/analysis MESH: Ecology MESH: Flow Cytometry MESH: Genes rRNA MESH: Genotype MESH: In Situ Hybridization Fluorescence MESH: Molecular Sequence Data MESH: Oligonucleotide Probes/genetics MESH: Phylogeny MESH: RNA Ribosomal 16S/genetics MESH: Seawater/microbiology MESH: Sequence Analysis DNA MESH: Cyanobacteria/genetics MESH: Cyanobacteria/physiology [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology West, N. J. Schonhuber, W. A. Fuller, N. J. Amann, R. I. Rippka, R. Post, A. F. Scanlan, D. J. Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides |
topic_facet |
16S rRNA TSA oligonucleotide probes Red Sea cyanobacteria MESH: Colony Count Microbial MESH: Cyanobacteria/classification MESH: DNA Ribosomal/analysis MESH: Ecology MESH: Flow Cytometry MESH: Genes rRNA MESH: Genotype MESH: In Situ Hybridization Fluorescence MESH: Molecular Sequence Data MESH: Oligonucleotide Probes/genetics MESH: Phylogeny MESH: RNA Ribosomal 16S/genetics MESH: Seawater/microbiology MESH: Sequence Analysis DNA MESH: Cyanobacteria/genetics MESH: Cyanobacteria/physiology [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
International audience An in situ hybridization method was applied to the identification of marine cyanobacteria assignable to the genus Prochlorococcus using horseradish-peroxidase-labelled 16S rRNA-targeted oligonucleotide probes in combination with tyramide signal amplification (TSA). With this method very bright signals were obtained, in contrast to hybridizations with oligonucleotides monolabelled with fluorochromes, which failed to give positive signals. Genotype-specific oligonucleotides for high light (HL)- and low light (LL)-adapted members of this genus were identified by 16S rRNA sequence analyses and their specificities confirmed in whole-cell hybridizations with cultured strains of Prochlorococcus marinus Chisholm et al., 1992 R8 , Prochlorococcus sp. and Synechococcus sp. In situ hybridization of these genotype-specific probes to field samples from stratified water bodies collected in the North Atlantic Ocean and the Red Sea allowed a rapid assessment of the abundance and spatial distribution of HL- and LL-adapted Prochlorococcus. In both oceanic regions the LL-adapted Prochlorococcus populations were localized in deeper water whereas the HL-adapted Prochlorococcus populations were not only distinct in each region but also exhibited strikingly different depth distributions, HLI being confined to shallow water in the North Atlantic, in contrast to HLII, which was present throughout the water column in the Red Sea. |
author2 |
University of Warwick Coventry Max Planck Institute for Marine Microbiology Max-Planck-Gesellschaft Physiologie microbienne Institut Pasteur Paris -Centre National de la Recherche Scientifique (CNRS) The Interuniversity Institute for marine Science in Eilat This work was supported in part by NERC grant GST/02/1081 (PRIME thematic programme), NERC grant GR3/11606 and by the EU grants MAS3-CT97-0128 (PROMOLEC) and EVK3-CT-1999-00021 (PICODIV). W. A. Schönhuber, R. I. Amann and R. Rippka were supported in part by contract BIO4-CT96-0256 (BASIC) of the European programme BIOTECH (Life Sciences and Technologies, Biotechnology Programme, 1994–1998). R. Rippka also acknowledges support by the Institut Pasteur and the Centre National de la Recherche Scientifique (CNRS, URA 2172). W. A. Schönhuber held a Marie Curie Fellowship from the EC (contract no. EVK1-CT1999-50002) with the Institut Pasteur as host institution. This is PRIME contribution number 126. D. J. Scanlan is a Royal Society University Research Fellow. |
format |
Article in Journal/Newspaper |
author |
West, N. J. Schonhuber, W. A. Fuller, N. J. Amann, R. I. Rippka, R. Post, A. F. Scanlan, D. J. |
author_facet |
West, N. J. Schonhuber, W. A. Fuller, N. J. Amann, R. I. Rippka, R. Post, A. F. Scanlan, D. J. |
author_sort |
West, N. J. |
title |
Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides |
title_short |
Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides |
title_full |
Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides |
title_fullStr |
Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides |
title_full_unstemmed |
Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides |
title_sort |
closely related prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16s rrna-targeted oligonucleotides |
publisher |
HAL CCSD |
publishDate |
2001 |
url |
https://hal.archives-ouvertes.fr/hal-01651552 https://doi.org/10.1099/00221287-147-7-1731 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 1350-0872 EISSN: 1465-2080 Microbiology https://hal.archives-ouvertes.fr/hal-01651552 Microbiology, Microbiology Society, 2001, 147 (Pt 7), pp.1731-1744. ⟨10.1099/00221287-147-7-1731⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/11429451 info:eu-repo/semantics/altIdentifier/doi/10.1099/00221287-147-7-1731 hal-01651552 https://hal.archives-ouvertes.fr/hal-01651552 PUBMED: 11429451 doi:10.1099/00221287-147-7-1731 |
op_doi |
https://doi.org/10.1099/00221287-147-7-1731 |
container_title |
Microbiology |
container_volume |
147 |
container_issue |
7 |
container_start_page |
1731 |
op_container_end_page |
1744 |
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1766129227829084160 |
spelling |
ftccsdartic:oai:HAL:hal-01651552v1 2023-05-15T17:31:34+02:00 Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides West, N. J. Schonhuber, W. A. Fuller, N. J. Amann, R. I. Rippka, R. Post, A. F. Scanlan, D. J. University of Warwick Coventry Max Planck Institute for Marine Microbiology Max-Planck-Gesellschaft Physiologie microbienne Institut Pasteur Paris -Centre National de la Recherche Scientifique (CNRS) The Interuniversity Institute for marine Science in Eilat This work was supported in part by NERC grant GST/02/1081 (PRIME thematic programme), NERC grant GR3/11606 and by the EU grants MAS3-CT97-0128 (PROMOLEC) and EVK3-CT-1999-00021 (PICODIV). W. A. Schönhuber, R. I. Amann and R. Rippka were supported in part by contract BIO4-CT96-0256 (BASIC) of the European programme BIOTECH (Life Sciences and Technologies, Biotechnology Programme, 1994–1998). R. Rippka also acknowledges support by the Institut Pasteur and the Centre National de la Recherche Scientifique (CNRS, URA 2172). W. A. Schönhuber held a Marie Curie Fellowship from the EC (contract no. EVK1-CT1999-50002) with the Institut Pasteur as host institution. This is PRIME contribution number 126. D. J. Scanlan is a Royal Society University Research Fellow. 2001-07 https://hal.archives-ouvertes.fr/hal-01651552 https://doi.org/10.1099/00221287-147-7-1731 en eng HAL CCSD Microbiology Society info:eu-repo/semantics/altIdentifier/pmid/11429451 info:eu-repo/semantics/altIdentifier/doi/10.1099/00221287-147-7-1731 hal-01651552 https://hal.archives-ouvertes.fr/hal-01651552 PUBMED: 11429451 doi:10.1099/00221287-147-7-1731 ISSN: 1350-0872 EISSN: 1465-2080 Microbiology https://hal.archives-ouvertes.fr/hal-01651552 Microbiology, Microbiology Society, 2001, 147 (Pt 7), pp.1731-1744. ⟨10.1099/00221287-147-7-1731⟩ 16S rRNA TSA oligonucleotide probes Red Sea cyanobacteria MESH: Colony Count Microbial MESH: Cyanobacteria/classification MESH: DNA Ribosomal/analysis MESH: Ecology MESH: Flow Cytometry MESH: Genes rRNA MESH: Genotype MESH: In Situ Hybridization Fluorescence MESH: Molecular Sequence Data MESH: Oligonucleotide Probes/genetics MESH: Phylogeny MESH: RNA Ribosomal 16S/genetics MESH: Seawater/microbiology MESH: Sequence Analysis DNA MESH: Cyanobacteria/genetics MESH: Cyanobacteria/physiology [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2001 ftccsdartic https://doi.org/10.1099/00221287-147-7-1731 2021-10-24T07:42:36Z International audience An in situ hybridization method was applied to the identification of marine cyanobacteria assignable to the genus Prochlorococcus using horseradish-peroxidase-labelled 16S rRNA-targeted oligonucleotide probes in combination with tyramide signal amplification (TSA). With this method very bright signals were obtained, in contrast to hybridizations with oligonucleotides monolabelled with fluorochromes, which failed to give positive signals. Genotype-specific oligonucleotides for high light (HL)- and low light (LL)-adapted members of this genus were identified by 16S rRNA sequence analyses and their specificities confirmed in whole-cell hybridizations with cultured strains of Prochlorococcus marinus Chisholm et al., 1992 R8 , Prochlorococcus sp. and Synechococcus sp. In situ hybridization of these genotype-specific probes to field samples from stratified water bodies collected in the North Atlantic Ocean and the Red Sea allowed a rapid assessment of the abundance and spatial distribution of HL- and LL-adapted Prochlorococcus. In both oceanic regions the LL-adapted Prochlorococcus populations were localized in deeper water whereas the HL-adapted Prochlorococcus populations were not only distinct in each region but also exhibited strikingly different depth distributions, HLI being confined to shallow water in the North Atlantic, in contrast to HLII, which was present throughout the water column in the Red Sea. Article in Journal/Newspaper North Atlantic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Microbiology 147 7 1731 1744 |