Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter
International audience The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low-energy conditions of the winter, but little is known about the specific phy...
Published in: | Frontiers in Microbiology |
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Main Authors: | , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2013
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Subjects: | |
Online Access: | https://hal.sorbonne-universite.fr/hal-01561031 https://hal.sorbonne-universite.fr/hal-01561031/document https://hal.sorbonne-universite.fr/hal-01561031/file/fmicb-04-00118.pdf https://doi.org/10.3389/fmicb.2013.00118 |
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ftccsdartic:oai:HAL:hal-01561031v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
BrdU Arctic Ocean Colwellia bacteria activity [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
BrdU Arctic Ocean Colwellia bacteria activity [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Galand, Pierre Alonso-Saez, Laura Bertilsson, Stefan Lovejoy, Connie Casamayor, Emilio O. Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
topic_facet |
BrdU Arctic Ocean Colwellia bacteria activity [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low-energy conditions of the winter, but little is known about the specific phylotypes that have the potential to survive and grow in such harsh environment. In this study, we aimed at identifying actively growing ribotypes in winter Arctic Ocean seawater cultures by experimental incubations with the thymidine analog bromodeoxyuridine (BrdU), followed by immunocapturing, terminal restriction fragment length polymorphism fingerprinting, cloning, and sequencing the 16S rRNA gene. We incubated water collected at different months over the Arctic winter and showed that the actively growing bacterial fraction, taking up BrdU, represented only a subset of the total community. Among the BrdU-labeled bacterial taxa we identified the Flavobacteria Polaribacter, the Alphaproteobacteria SAR11, the Gammaproteobacteria Arctic 96B-16 cluster and, predominately, members of Colwellia spp. Interestingly, Colwellia sequences formed three clusters (93 and 97% pairwise 16S rRNA identity) that contributed in contrasting ways to the active communities in the incubations. Polaribacter, Arctic 96B-16 and one cluster of Colwellia were more abundant in the active community represented by the BrdU-labeled DNA. In contrast, SAR11 and two other Colwellia clusters were underrepresented in the BrdU-labeled community compared to total communities. Despite the limitation of the long incubations needed to label slow growing arctic communities, the BrdU approach revealed the potential for active growth in low-energy conditions in some relevant groups of polar bacteria, including Polaribacter and Arctic 96B-16. Moreover, under similar incubation conditions, the growth of different Colwellia ribotypes varied, suggesting that related clusters of Colwellia may have distinct metabolic features. |
author2 |
Centre d'Estudis Avançats de Blanes (CEAB) Consejo Superior de Investigaciones Científicas Madrid (CSIC) Laboratoire d'Ecogéochimie des environnements benthiques (LECOB) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Uppsala Universitet Uppsala Centro Oceanográfico de Gijón (IEO) Université Laval Québec (ULaval) |
format |
Article in Journal/Newspaper |
author |
Galand, Pierre Alonso-Saez, Laura Bertilsson, Stefan Lovejoy, Connie Casamayor, Emilio O. |
author_facet |
Galand, Pierre Alonso-Saez, Laura Bertilsson, Stefan Lovejoy, Connie Casamayor, Emilio O. |
author_sort |
Galand, Pierre |
title |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_short |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_full |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_fullStr |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_full_unstemmed |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_sort |
contrasting activity patterns determined by brdu incorporation in bacterial ribotypes from the arctic ocean in winter |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.sorbonne-universite.fr/hal-01561031 https://hal.sorbonne-universite.fr/hal-01561031/document https://hal.sorbonne-universite.fr/hal-01561031/file/fmicb-04-00118.pdf https://doi.org/10.3389/fmicb.2013.00118 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal.sorbonne-universite.fr/hal-01561031 Frontiers in Microbiology, Frontiers Media, 2013, 4, pp.118. ⟨10.3389/fmicb.2013.00118⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2013.00118 hal-01561031 https://hal.sorbonne-universite.fr/hal-01561031 https://hal.sorbonne-universite.fr/hal-01561031/document https://hal.sorbonne-universite.fr/hal-01561031/file/fmicb-04-00118.pdf doi:10.3389/fmicb.2013.00118 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3389/fmicb.2013.00118 |
container_title |
Frontiers in Microbiology |
container_volume |
4 |
_version_ |
1766308088907825152 |
spelling |
ftccsdartic:oai:HAL:hal-01561031v1 2023-05-15T14:35:13+02:00 Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter Galand, Pierre Alonso-Saez, Laura Bertilsson, Stefan Lovejoy, Connie Casamayor, Emilio O. Centre d'Estudis Avançats de Blanes (CEAB) Consejo Superior de Investigaciones Científicas Madrid (CSIC) Laboratoire d'Ecogéochimie des environnements benthiques (LECOB) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Uppsala Universitet Uppsala Centro Oceanográfico de Gijón (IEO) Université Laval Québec (ULaval) 2013 https://hal.sorbonne-universite.fr/hal-01561031 https://hal.sorbonne-universite.fr/hal-01561031/document https://hal.sorbonne-universite.fr/hal-01561031/file/fmicb-04-00118.pdf https://doi.org/10.3389/fmicb.2013.00118 en eng HAL CCSD Frontiers Media info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2013.00118 hal-01561031 https://hal.sorbonne-universite.fr/hal-01561031 https://hal.sorbonne-universite.fr/hal-01561031/document https://hal.sorbonne-universite.fr/hal-01561031/file/fmicb-04-00118.pdf doi:10.3389/fmicb.2013.00118 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal.sorbonne-universite.fr/hal-01561031 Frontiers in Microbiology, Frontiers Media, 2013, 4, pp.118. ⟨10.3389/fmicb.2013.00118⟩ BrdU Arctic Ocean Colwellia bacteria activity [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.3389/fmicb.2013.00118 2021-09-12T00:28:06Z International audience The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low-energy conditions of the winter, but little is known about the specific phylotypes that have the potential to survive and grow in such harsh environment. In this study, we aimed at identifying actively growing ribotypes in winter Arctic Ocean seawater cultures by experimental incubations with the thymidine analog bromodeoxyuridine (BrdU), followed by immunocapturing, terminal restriction fragment length polymorphism fingerprinting, cloning, and sequencing the 16S rRNA gene. We incubated water collected at different months over the Arctic winter and showed that the actively growing bacterial fraction, taking up BrdU, represented only a subset of the total community. Among the BrdU-labeled bacterial taxa we identified the Flavobacteria Polaribacter, the Alphaproteobacteria SAR11, the Gammaproteobacteria Arctic 96B-16 cluster and, predominately, members of Colwellia spp. Interestingly, Colwellia sequences formed three clusters (93 and 97% pairwise 16S rRNA identity) that contributed in contrasting ways to the active communities in the incubations. Polaribacter, Arctic 96B-16 and one cluster of Colwellia were more abundant in the active community represented by the BrdU-labeled DNA. In contrast, SAR11 and two other Colwellia clusters were underrepresented in the BrdU-labeled community compared to total communities. Despite the limitation of the long incubations needed to label slow growing arctic communities, the BrdU approach revealed the potential for active growth in low-energy conditions in some relevant groups of polar bacteria, including Polaribacter and Arctic 96B-16. Moreover, under similar incubation conditions, the growth of different Colwellia ribotypes varied, suggesting that related clusters of Colwellia may have distinct metabolic features. Article in Journal/Newspaper Arctic Arctic Ocean Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic Arctic Ocean Frontiers in Microbiology 4 |