Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic)
International audience We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communitie...
Published in: | Biogeosciences |
---|---|
Main Authors: | , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2013
|
Subjects: | |
Online Access: | https://hal.sorbonne-universite.fr/hal-01548259 https://hal.sorbonne-universite.fr/hal-01548259/document https://hal.sorbonne-universite.fr/hal-01548259/file/bg-10-2747-2013.pdf https://doi.org/10.5194/bg-10-2747-2013 |
id |
ftccsdartic:oai:HAL:hal-01548259v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:hal-01548259v1 2023-05-15T15:01:53+02:00 Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) Ortega-Retuerta, E. Joux, F. Jeffrey, W. H. Ghiglione, J. F. Laboratoire d'Océanographie Microbienne (LOMIC) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Center for Environmental Diagnostics and Bioremediation Pensacola (UWF University of West Florida Pensacola (UWF) 2013 https://hal.sorbonne-universite.fr/hal-01548259 https://hal.sorbonne-universite.fr/hal-01548259/document https://hal.sorbonne-universite.fr/hal-01548259/file/bg-10-2747-2013.pdf https://doi.org/10.5194/bg-10-2747-2013 en eng HAL CCSD European Geosciences Union info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-10-2747-2013 hal-01548259 https://hal.sorbonne-universite.fr/hal-01548259 https://hal.sorbonne-universite.fr/hal-01548259/document https://hal.sorbonne-universite.fr/hal-01548259/file/bg-10-2747-2013.pdf doi:10.5194/bg-10-2747-2013 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1726-4170 EISSN: 1726-4189 Biogeosciences https://hal.sorbonne-universite.fr/hal-01548259 Biogeosciences, European Geosciences Union, 2013, 10 (4), pp.2747 - 2759. ⟨10.5194/bg-10-2747-2013⟩ [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.5194/bg-10-2747-2013 2021-05-22T23:18:23Z International audience We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis–single-strand conformation poly-morphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different , suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a. Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7 % similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally , for the coast and river samples and both PA and FL fractions, Betaproteobacteria, Alphaproteobacteria and Acti-nobacteria were dominant. These results highlight the coexistence of particle specialists and generalists and the role of particle quality in structuring bacterial communities in the area. These results may also serve as a basis ... Article in Journal/Newspaper Arctic Arctic Ocean Beaufort Sea Mackenzie river Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic Arctic Ocean Mackenzie River Biogeosciences 10 4 2747 2759 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere |
spellingShingle |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere Ortega-Retuerta, E. Joux, F. Jeffrey, W. H. Ghiglione, J. F. Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
topic_facet |
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere |
description |
International audience We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis–single-strand conformation poly-morphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different , suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a. Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7 % similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally , for the coast and river samples and both PA and FL fractions, Betaproteobacteria, Alphaproteobacteria and Acti-nobacteria were dominant. These results highlight the coexistence of particle specialists and generalists and the role of particle quality in structuring bacterial communities in the area. These results may also serve as a basis ... |
author2 |
Laboratoire d'Océanographie Microbienne (LOMIC) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Center for Environmental Diagnostics and Bioremediation Pensacola (UWF University of West Florida Pensacola (UWF) |
format |
Article in Journal/Newspaper |
author |
Ortega-Retuerta, E. Joux, F. Jeffrey, W. H. Ghiglione, J. F. |
author_facet |
Ortega-Retuerta, E. Joux, F. Jeffrey, W. H. Ghiglione, J. F. |
author_sort |
Ortega-Retuerta, E. |
title |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_short |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_full |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_fullStr |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_full_unstemmed |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_sort |
spatial variability of particle-attached and free-living bacterial diversity in surface waters from the mackenzie river to the beaufort sea (canadian arctic) |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.sorbonne-universite.fr/hal-01548259 https://hal.sorbonne-universite.fr/hal-01548259/document https://hal.sorbonne-universite.fr/hal-01548259/file/bg-10-2747-2013.pdf https://doi.org/10.5194/bg-10-2747-2013 |
geographic |
Arctic Arctic Ocean Mackenzie River |
geographic_facet |
Arctic Arctic Ocean Mackenzie River |
genre |
Arctic Arctic Ocean Beaufort Sea Mackenzie river |
genre_facet |
Arctic Arctic Ocean Beaufort Sea Mackenzie river |
op_source |
ISSN: 1726-4170 EISSN: 1726-4189 Biogeosciences https://hal.sorbonne-universite.fr/hal-01548259 Biogeosciences, European Geosciences Union, 2013, 10 (4), pp.2747 - 2759. ⟨10.5194/bg-10-2747-2013⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-10-2747-2013 hal-01548259 https://hal.sorbonne-universite.fr/hal-01548259 https://hal.sorbonne-universite.fr/hal-01548259/document https://hal.sorbonne-universite.fr/hal-01548259/file/bg-10-2747-2013.pdf doi:10.5194/bg-10-2747-2013 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.5194/bg-10-2747-2013 |
container_title |
Biogeosciences |
container_volume |
10 |
container_issue |
4 |
container_start_page |
2747 |
op_container_end_page |
2759 |
_version_ |
1766333880566022144 |