Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas
International audience AbstractThis study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and...
Published in: | Veterinary Research |
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Language: | English |
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Online Access: | https://hal.archives-ouvertes.fr/hal-01528062 https://hal.archives-ouvertes.fr/hal-01528062/document https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_Article_438.pdf https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_438_MOESM1_ESM.pdf https://doi.org/10.1186/s13567-017-0438-1 |
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ftccsdartic:oai:HAL:hal-01528062v1 2023-05-15T15:58:47+02:00 Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas Travers, Marie-Agnès Tourbiez, Delphine Parizadeh, Leïla Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie 2017-12 https://hal.archives-ouvertes.fr/hal-01528062 https://hal.archives-ouvertes.fr/hal-01528062/document https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_Article_438.pdf https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_438_MOESM1_ESM.pdf https://doi.org/10.1186/s13567-017-0438-1 en eng HAL CCSD BioMed Central info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-017-0438-1 hal-01528062 https://hal.archives-ouvertes.fr/hal-01528062 https://hal.archives-ouvertes.fr/hal-01528062/document https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_Article_438.pdf https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_438_MOESM1_ESM.pdf doi:10.1186/s13567-017-0438-1 info:eu-repo/semantics/OpenAccess ISSN: 0928-4249 EISSN: 1297-9716 Veterinary Research https://hal.archives-ouvertes.fr/hal-01528062 Veterinary Research, BioMed Central, 2017, 48 (1), pp.32. ⟨10.1186/s13567-017-0438-1⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2017 ftccsdartic https://doi.org/10.1186/s13567-017-0438-1 2021-02-28T01:28:56Z International audience AbstractThis study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Veterinary Research 48 1 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
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[SDV]Life Sciences [q-bio] Travers, Marie-Agnès Tourbiez, Delphine Parizadeh, Leïla Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
International audience AbstractThis study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. |
format |
Article in Journal/Newspaper |
author |
Travers, Marie-Agnès Tourbiez, Delphine Parizadeh, Leïla Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie |
author_facet |
Travers, Marie-Agnès Tourbiez, Delphine Parizadeh, Leïla Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie |
author_sort |
Travers, Marie-Agnès |
title |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_short |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_sort |
several strains, one disease: experimental investigation of vibrio aestuarianus infection parameters in the pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.archives-ouvertes.fr/hal-01528062 https://hal.archives-ouvertes.fr/hal-01528062/document https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_Article_438.pdf https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_438_MOESM1_ESM.pdf https://doi.org/10.1186/s13567-017-0438-1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0928-4249 EISSN: 1297-9716 Veterinary Research https://hal.archives-ouvertes.fr/hal-01528062 Veterinary Research, BioMed Central, 2017, 48 (1), pp.32. ⟨10.1186/s13567-017-0438-1⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-017-0438-1 hal-01528062 https://hal.archives-ouvertes.fr/hal-01528062 https://hal.archives-ouvertes.fr/hal-01528062/document https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_Article_438.pdf https://hal.archives-ouvertes.fr/hal-01528062/file/13567_2017_438_MOESM1_ESM.pdf doi:10.1186/s13567-017-0438-1 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s13567-017-0438-1 |
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Veterinary Research |
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48 |
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1 |
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1766394547375439872 |