Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L
International audience Maternal mRNA governs early embryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in European eel, Anguilla anguilla. Previous studies have shown that quantities of the maternal gene...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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ftccsdartic:oai:HAL:hal-01483245v1 2023-05-15T13:27:12+02:00 Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L Rozenfeld, Christoffer Butts, Ian A. E. Tomkiewicz, Jonna Zambonino-Infante, Jose-Luis Mazurais, David DTU Aqua, National Institute of Aquatic Resources Technical University of Denmark Lyngby (DTU) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 245257,EC:FP7:KBBE,FP7-KBBE-2009-3,PRO-EEL(2010) 2016-01 https://hal.archives-ouvertes.fr/hal-01483245 https://doi.org/10.1016/j.cbpa.2015.09.011 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2015.09.011 info:eu-repo/grantAgreement/EC/FP7/245257/EU/Reproduction of European Eel: Towards a Self-sustained Aquaculture/PRO-EEL hal-01483245 https://hal.archives-ouvertes.fr/hal-01483245 doi:10.1016/j.cbpa.2015.09.011 ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal.archives-ouvertes.fr/hal-01483245 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2016, 191, pp.59-65. ⟨10.1016/j.cbpa.2015.09.011⟩ Teleost embryogenesis ACL aquaculture Maternal and embryonic mRNA qPCR Segmentation stage [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2016 ftccsdartic https://doi.org/10.1016/j.cbpa.2015.09.011 2021-12-19T02:37:37Z International audience Maternal mRNA governs early embryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in European eel, Anguilla anguilla. Previous studies have shown that quantities of the maternal gene products β-tubulin, insulin-like growth factor 2 (igf2), nucleoplasmin (npm2), prohibitin 2 (phb2), phosphatidylinositol glycan biosynthesis class F protein 5 (pigf5), and carnitine O-palmitoyltransferase liver isoform-like 1 (cpt1) are associated with embryonic developmental competence in other teleosts. Here, the relations between relative mRNA abundance of these genes in eggs and/or embryos and egg quality, was studied and analyzed. We compared egg quality of the two groups: i) batches with hatching and ii) batches with no hatching. Results showed no significant differences in relative mRNA abundance between the hatch and no hatching groups for any of the selected genes at 0, 2.5, and 5 HPF. However, at 30 HPF the hatch group showed significantly higher abundance of cpt1a, cpt1b, β-tubulin, phb2, and pigf5 transcripts than the no hatch group. Therefore, these results indicate that up-regulation of the transcription of these genes in European eel after the mid-blastula transition, may be needed to sustain embryonic development and hatching success. Article in Journal/Newspaper Anguilla anguilla Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 191 59 65 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Teleost embryogenesis ACL aquaculture Maternal and embryonic mRNA qPCR Segmentation stage [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Teleost embryogenesis ACL aquaculture Maternal and embryonic mRNA qPCR Segmentation stage [SDE.BE]Environmental Sciences/Biodiversity and Ecology Rozenfeld, Christoffer Butts, Ian A. E. Tomkiewicz, Jonna Zambonino-Infante, Jose-Luis Mazurais, David Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L |
topic_facet |
Teleost embryogenesis ACL aquaculture Maternal and embryonic mRNA qPCR Segmentation stage [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Maternal mRNA governs early embryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in European eel, Anguilla anguilla. Previous studies have shown that quantities of the maternal gene products β-tubulin, insulin-like growth factor 2 (igf2), nucleoplasmin (npm2), prohibitin 2 (phb2), phosphatidylinositol glycan biosynthesis class F protein 5 (pigf5), and carnitine O-palmitoyltransferase liver isoform-like 1 (cpt1) are associated with embryonic developmental competence in other teleosts. Here, the relations between relative mRNA abundance of these genes in eggs and/or embryos and egg quality, was studied and analyzed. We compared egg quality of the two groups: i) batches with hatching and ii) batches with no hatching. Results showed no significant differences in relative mRNA abundance between the hatch and no hatching groups for any of the selected genes at 0, 2.5, and 5 HPF. However, at 30 HPF the hatch group showed significantly higher abundance of cpt1a, cpt1b, β-tubulin, phb2, and pigf5 transcripts than the no hatch group. Therefore, these results indicate that up-regulation of the transcription of these genes in European eel after the mid-blastula transition, may be needed to sustain embryonic development and hatching success. |
author2 |
DTU Aqua, National Institute of Aquatic Resources Technical University of Denmark Lyngby (DTU) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 245257,EC:FP7:KBBE,FP7-KBBE-2009-3,PRO-EEL(2010) |
format |
Article in Journal/Newspaper |
author |
Rozenfeld, Christoffer Butts, Ian A. E. Tomkiewicz, Jonna Zambonino-Infante, Jose-Luis Mazurais, David |
author_facet |
Rozenfeld, Christoffer Butts, Ian A. E. Tomkiewicz, Jonna Zambonino-Infante, Jose-Luis Mazurais, David |
author_sort |
Rozenfeld, Christoffer |
title |
Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L |
title_short |
Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L |
title_full |
Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L |
title_fullStr |
Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L |
title_full_unstemmed |
Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L |
title_sort |
abundance of specific mrna transcripts impacts hatching success in european eel, anguilla anguilla l |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.archives-ouvertes.fr/hal-01483245 https://doi.org/10.1016/j.cbpa.2015.09.011 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal.archives-ouvertes.fr/hal-01483245 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2016, 191, pp.59-65. ⟨10.1016/j.cbpa.2015.09.011⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2015.09.011 info:eu-repo/grantAgreement/EC/FP7/245257/EU/Reproduction of European Eel: Towards a Self-sustained Aquaculture/PRO-EEL hal-01483245 https://hal.archives-ouvertes.fr/hal-01483245 doi:10.1016/j.cbpa.2015.09.011 |
op_doi |
https://doi.org/10.1016/j.cbpa.2015.09.011 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
191 |
container_start_page |
59 |
op_container_end_page |
65 |
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