Estimating microbial populations by flow cytometry: Comparison between instruments
International audience For almost three decades, flow cytometry has allowed researchers to investigate ocean planktonic communities using size and cell fluorescence properties. However, oceanographic applications must face two constraints. First, when dealing with marine microbes, instruments must b...
Published in: | Limnology and Oceanography: Methods |
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Online Access: | https://hal.sorbonne-universite.fr/hal-01482199 https://hal.sorbonne-universite.fr/hal-01482199/document https://hal.sorbonne-universite.fr/hal-01482199/file/Gerikas_Ribeiro_2016_Estimating.pdf https://doi.org/10.1002/lom3.10135 |
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ftccsdartic:oai:HAL:hal-01482199v1 2024-02-27T08:45:28+00:00 Estimating microbial populations by flow cytometry: Comparison between instruments Gérikas Ribeiro, Catherine Marie, Dominique Lopes dos Santos, Adriana Pereira Brandini, Frederico Vaulot, Daniel Departamento de Oceanografia Biologica, Instituto Oceanografico, Universidade de Sao Paulo Diversité et Interactions au sein du Plancton Océanique (DIPO) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2017 https://hal.sorbonne-universite.fr/hal-01482199 https://hal.sorbonne-universite.fr/hal-01482199/document https://hal.sorbonne-universite.fr/hal-01482199/file/Gerikas_Ribeiro_2016_Estimating.pdf https://doi.org/10.1002/lom3.10135 en eng HAL CCSD Association for the Sciences of Limnology and Oceanography info:eu-repo/semantics/altIdentifier/doi/10.1002/lom3.10135 hal-01482199 https://hal.sorbonne-universite.fr/hal-01482199 https://hal.sorbonne-universite.fr/hal-01482199/document https://hal.sorbonne-universite.fr/hal-01482199/file/Gerikas_Ribeiro_2016_Estimating.pdf doi:10.1002/lom3.10135 info:eu-repo/semantics/OpenAccess ISSN: 1541-5856 Limnology and Oceanography: Methods https://hal.sorbonne-universite.fr/hal-01482199 Limnology and Oceanography: Methods, 2017, 14 (11), pp.750 - 758. ⟨10.1002/lom3.10135⟩ [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2017 ftccsdartic https://doi.org/10.1002/lom3.10135 2024-01-28T03:43:08Z International audience For almost three decades, flow cytometry has allowed researchers to investigate ocean planktonic communities using size and cell fluorescence properties. However, oceanographic applications must face two constraints. First, when dealing with marine microbes, instruments must be sensitive because these organisms are very small and with low fluorescence. Second, instruments must be portable to be used on board ships. We compared the performance of two instruments, the BD FACSCanto™ and BD Accuri™ C6. The former is an expensive laboratory-based instrument which has a very good sensitivity, whilst the latter is less sensitive but presents critical advantages for field studies (easy handling and transportation, relatively low cost). We have analyzed 102 samples from the South Atlantic Ocean from three transects off Brazil, within the euphotic zone. We compared cell abundance of heterotrophic bacteria, Prochlorococcus and Synechococcus, as well as photosynthetic pico- and nano-eukaryotes. Heterotrophic bacteria, pico- and nano-eukaryotes could be easily detected with both cytometers. Prochlorococcus and Synechococcus populations were severely under-estimated with the BD Accuri™ C6, particularly for samples from the well-lit layers of the water column. Correction of abundance data using previously suggested approaches was not sufficient to fully compensate for the low sensibility. Our data suggest that the BD Accuri™ C6 is suitable for counting marine bacteria and photosynthetic eukaryotes, but not Prochlorococcus and Synechococcus. Article in Journal/Newspaper South Atlantic Ocean Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Limnology and Oceanography: Methods 14 11 750 758 |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
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[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Gérikas Ribeiro, Catherine Marie, Dominique Lopes dos Santos, Adriana Pereira Brandini, Frederico Vaulot, Daniel Estimating microbial populations by flow cytometry: Comparison between instruments |
topic_facet |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience For almost three decades, flow cytometry has allowed researchers to investigate ocean planktonic communities using size and cell fluorescence properties. However, oceanographic applications must face two constraints. First, when dealing with marine microbes, instruments must be sensitive because these organisms are very small and with low fluorescence. Second, instruments must be portable to be used on board ships. We compared the performance of two instruments, the BD FACSCanto™ and BD Accuri™ C6. The former is an expensive laboratory-based instrument which has a very good sensitivity, whilst the latter is less sensitive but presents critical advantages for field studies (easy handling and transportation, relatively low cost). We have analyzed 102 samples from the South Atlantic Ocean from three transects off Brazil, within the euphotic zone. We compared cell abundance of heterotrophic bacteria, Prochlorococcus and Synechococcus, as well as photosynthetic pico- and nano-eukaryotes. Heterotrophic bacteria, pico- and nano-eukaryotes could be easily detected with both cytometers. Prochlorococcus and Synechococcus populations were severely under-estimated with the BD Accuri™ C6, particularly for samples from the well-lit layers of the water column. Correction of abundance data using previously suggested approaches was not sufficient to fully compensate for the low sensibility. Our data suggest that the BD Accuri™ C6 is suitable for counting marine bacteria and photosynthetic eukaryotes, but not Prochlorococcus and Synechococcus. |
author2 |
Departamento de Oceanografia Biologica, Instituto Oceanografico, Universidade de Sao Paulo Diversité et Interactions au sein du Plancton Océanique (DIPO) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Gérikas Ribeiro, Catherine Marie, Dominique Lopes dos Santos, Adriana Pereira Brandini, Frederico Vaulot, Daniel |
author_facet |
Gérikas Ribeiro, Catherine Marie, Dominique Lopes dos Santos, Adriana Pereira Brandini, Frederico Vaulot, Daniel |
author_sort |
Gérikas Ribeiro, Catherine |
title |
Estimating microbial populations by flow cytometry: Comparison between instruments |
title_short |
Estimating microbial populations by flow cytometry: Comparison between instruments |
title_full |
Estimating microbial populations by flow cytometry: Comparison between instruments |
title_fullStr |
Estimating microbial populations by flow cytometry: Comparison between instruments |
title_full_unstemmed |
Estimating microbial populations by flow cytometry: Comparison between instruments |
title_sort |
estimating microbial populations by flow cytometry: comparison between instruments |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.sorbonne-universite.fr/hal-01482199 https://hal.sorbonne-universite.fr/hal-01482199/document https://hal.sorbonne-universite.fr/hal-01482199/file/Gerikas_Ribeiro_2016_Estimating.pdf https://doi.org/10.1002/lom3.10135 |
genre |
South Atlantic Ocean |
genre_facet |
South Atlantic Ocean |
op_source |
ISSN: 1541-5856 Limnology and Oceanography: Methods https://hal.sorbonne-universite.fr/hal-01482199 Limnology and Oceanography: Methods, 2017, 14 (11), pp.750 - 758. ⟨10.1002/lom3.10135⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/lom3.10135 hal-01482199 https://hal.sorbonne-universite.fr/hal-01482199 https://hal.sorbonne-universite.fr/hal-01482199/document https://hal.sorbonne-universite.fr/hal-01482199/file/Gerikas_Ribeiro_2016_Estimating.pdf doi:10.1002/lom3.10135 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1002/lom3.10135 |
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Limnology and Oceanography: Methods |
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14 |
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11 |
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750 |
op_container_end_page |
758 |
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