Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles
International audience Notodiscus hookeri is the only native land snail of the sub-Antarctic present in Crozet Archipelago. Two shell ecophenotypes co-exist on Possession Island and feed exclusively on lichens [1] that are symbiotic organisms widely distributed in sub-Antarctic islands. To improve o...
Published in: | Planta Medica |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2016
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Online Access: | https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446729 https://doi.org/10.1055/s-0036-1596670 |
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ftccsdartic:oai:HAL:hal-01446729v1 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Sub-antarctic lichens metabolites profiling DART-MS Notodiscus hookeri chemical ecology [CHIM]Chemical Sciences |
spellingShingle |
Sub-antarctic lichens metabolites profiling DART-MS Notodiscus hookeri chemical ecology [CHIM]Chemical Sciences Gadea, Alice Lohézic-Le Dévéhat, Françoise Le Lamer, Anne-Cécile Le Pogam, P. Ertz, Damien Charrier, Maryvonne Boustie, Joël Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
topic_facet |
Sub-antarctic lichens metabolites profiling DART-MS Notodiscus hookeri chemical ecology [CHIM]Chemical Sciences |
description |
International audience Notodiscus hookeri is the only native land snail of the sub-Antarctic present in Crozet Archipelago. Two shell ecophenotypes co-exist on Possession Island and feed exclusively on lichens [1] that are symbiotic organisms widely distributed in sub-Antarctic islands. To improve our understanding of lichen-snail trophic interactions, the endemic tripartite lichen Argopsis friesiana was selected. The snail belonging to the organic ecophenotype occurs sympatrically with A. friesiana. Profiling and quantification of the major secondary metabolites in the whole lichen were carried out by LC-DAD-MS. Two main compounds were identified, the depside atranorin (8.2 mg g-1 DM) and the depsidone argopsin. Besides, a screening of primary metabolites was carried out to determine which compounds could be useful for the snail energetic demands. Glutamate, glutamine, gamma-aminobutyric acid and alpha-alanine were the main amino acids found in the lichen in low concentrations (0.4 mg·g-1 DM). Carbohydrates, including arabitol (10.2 mg g-1), mannitol (4.0.10-4 mg g-1) and sucrose (3.0.10-4 mg g-1), were also identified. In cultivation, N. hookeri snails were fed with fructified thalli of A. friesiana. Snails (n = 20) ate the podetia cortex and phyllocladia but they clearly avoided apothecia, the reproductive organs. This sectorial damage of the lichen by the snail suggested a differential distribution of the metabolites. Therefore, a profiling of secondary compounds was performed by Direct Analysis in Real Time – Mass Spectroscopy (DART-MS) [2] on each morphological part of the lichen (phyllocladia, apothecia, podetia and cephalodia). The identification and distribution of secondary metabolites, fatty acids, amino acids and carbohydrates should help us to determine whether metabolites affect snail feeding choice and which of them could be involved. |
author2 |
Institut des Sciences Chimiques de Rennes (ISCR) Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA) Ecosystèmes, biodiversité, évolution Rennes (ECOBIO) Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) Pharmacochimie et Biologie pour le Développement (PHARMA-DEV) Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599) Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC) Botanic Garden Meise |
format |
Article in Journal/Newspaper |
author |
Gadea, Alice Lohézic-Le Dévéhat, Françoise Le Lamer, Anne-Cécile Le Pogam, P. Ertz, Damien Charrier, Maryvonne Boustie, Joël |
author_facet |
Gadea, Alice Lohézic-Le Dévéhat, Françoise Le Lamer, Anne-Cécile Le Pogam, P. Ertz, Damien Charrier, Maryvonne Boustie, Joël |
author_sort |
Gadea, Alice |
title |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_short |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_full |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_fullStr |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_full_unstemmed |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_sort |
sectorial land snail damage to the lichen argopsis friesiana could be explained by metabolite profiles |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446729 https://doi.org/10.1055/s-0036-1596670 |
long_lat |
ENVELOPE(171.200,171.200,-71.867,-71.867) |
geographic |
Antarctic Possession Island |
geographic_facet |
Antarctic Possession Island |
genre |
Antarc* Antarctic Possession Island |
genre_facet |
Antarc* Antarctic Possession Island |
op_source |
ISSN: 0032-0943 EISSN: 1439-0221 Planta Medica https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446729 Planta Medica, Georg Thieme Verlag, 2016, 81 (S 01), pp.S1-S381. ⟨10.1055/s-0036-1596670⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0036-1596670 info:eu-repo/semantics/altIdentifier/pmid/27975871 hal-01446729 https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446729 doi:10.1055/s-0036-1596670 PUBMED: 27975871 |
op_doi |
https://doi.org/10.1055/s-0036-1596670 |
container_title |
Planta Medica |
container_volume |
81 |
container_issue |
S 01 |
container_start_page |
S1 |
op_container_end_page |
S381 |
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1766275431817805824 |
spelling |
ftccsdartic:oai:HAL:hal-01446729v1 2023-05-15T14:04:23+02:00 Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles Gadea, Alice Lohézic-Le Dévéhat, Françoise Le Lamer, Anne-Cécile Le Pogam, P. Ertz, Damien Charrier, Maryvonne Boustie, Joël Institut des Sciences Chimiques de Rennes (ISCR) Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA) Ecosystèmes, biodiversité, évolution Rennes (ECOBIO) Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) Pharmacochimie et Biologie pour le Développement (PHARMA-DEV) Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599) Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC) Botanic Garden Meise 2016 https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446729 https://doi.org/10.1055/s-0036-1596670 en eng HAL CCSD Georg Thieme Verlag info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0036-1596670 info:eu-repo/semantics/altIdentifier/pmid/27975871 hal-01446729 https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446729 doi:10.1055/s-0036-1596670 PUBMED: 27975871 ISSN: 0032-0943 EISSN: 1439-0221 Planta Medica https://hal-univ-rennes1.archives-ouvertes.fr/hal-01446729 Planta Medica, Georg Thieme Verlag, 2016, 81 (S 01), pp.S1-S381. ⟨10.1055/s-0036-1596670⟩ Sub-antarctic lichens metabolites profiling DART-MS Notodiscus hookeri chemical ecology [CHIM]Chemical Sciences info:eu-repo/semantics/article Journal articles 2016 ftccsdartic https://doi.org/10.1055/s-0036-1596670 2021-12-19T02:39:19Z International audience Notodiscus hookeri is the only native land snail of the sub-Antarctic present in Crozet Archipelago. Two shell ecophenotypes co-exist on Possession Island and feed exclusively on lichens [1] that are symbiotic organisms widely distributed in sub-Antarctic islands. To improve our understanding of lichen-snail trophic interactions, the endemic tripartite lichen Argopsis friesiana was selected. The snail belonging to the organic ecophenotype occurs sympatrically with A. friesiana. Profiling and quantification of the major secondary metabolites in the whole lichen were carried out by LC-DAD-MS. Two main compounds were identified, the depside atranorin (8.2 mg g-1 DM) and the depsidone argopsin. Besides, a screening of primary metabolites was carried out to determine which compounds could be useful for the snail energetic demands. Glutamate, glutamine, gamma-aminobutyric acid and alpha-alanine were the main amino acids found in the lichen in low concentrations (0.4 mg·g-1 DM). Carbohydrates, including arabitol (10.2 mg g-1), mannitol (4.0.10-4 mg g-1) and sucrose (3.0.10-4 mg g-1), were also identified. In cultivation, N. hookeri snails were fed with fructified thalli of A. friesiana. Snails (n = 20) ate the podetia cortex and phyllocladia but they clearly avoided apothecia, the reproductive organs. This sectorial damage of the lichen by the snail suggested a differential distribution of the metabolites. Therefore, a profiling of secondary compounds was performed by Direct Analysis in Real Time – Mass Spectroscopy (DART-MS) [2] on each morphological part of the lichen (phyllocladia, apothecia, podetia and cephalodia). The identification and distribution of secondary metabolites, fatty acids, amino acids and carbohydrates should help us to determine whether metabolites affect snail feeding choice and which of them could be involved. Article in Journal/Newspaper Antarc* Antarctic Possession Island Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Antarctic Possession Island ENVELOPE(171.200,171.200,-71.867,-71.867) Planta Medica 81 S 01 S1 S381 |