Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas
International audience Mollusc shells are organic–inorganic biocomposites, arranged in a limited number of superimposed calcified layers that generally exhibit very different organization of their crystallites. Because of their attractive mechanical and crystallographic properties, these shell layer...
Published in: | Marine Biology |
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Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2016
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Online Access: | https://hal.sorbonne-universite.fr/hal-01400675 https://hal.sorbonne-universite.fr/hal-01400675/document https://hal.sorbonne-universite.fr/hal-01400675/file/Mouchi_2016_Chalky_versus.pdf https://doi.org/10.1007/s00227-016-3040-6 |
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English |
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[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU]Sciences of the Universe [physics]/Earth Sciences |
spellingShingle |
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU]Sciences of the Universe [physics]/Earth Sciences Mouchi, Vincent Lartaud, Franck Guichard, Nathalie Immel, Françoise De Rafélis, Marc Broussard, Cédric Crowley, Quentin G. Marin, Frédéric Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas |
topic_facet |
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU]Sciences of the Universe [physics]/Earth Sciences |
description |
International audience Mollusc shells are organic–inorganic biocomposites, arranged in a limited number of superimposed calcified layers that generally exhibit very different organization of their crystallites. Because of their attractive mechanical and crystallographic properties, these shell layers have been the focus of several physical and biochemical characterizations. In particular, recent proteomic data obtained from individual layers suggest that their protein contents are different. However, the direct visual evidence that some macromolecular components are layer-specific is rather tenuous. This paper is based on a non-conventional immunogold labelling approach to localize proteins in the shell of the edible oyster Crassostrea gigas. The shell microstructure of this model organism is predominantly composed of foliated calcite, interspersed by discontinuous pockets of ‘chalky layers’, a porous microstructure typical of bivalves of the ostreid family. By developing a polyclonal antibody (in two rats) elicited against a proteinaceous shell fraction, we obtained differential staining of the two microstructures. We assert that our labelling is microstructure discriminant. The difference in labelling of the two shell microstructures suggests either that they are formed by a variation of the secretory repertoire of the shell-forming cells of the calcifying mantle epithelium or that the chalky layer may be formed via a completely different mechanism. Our results allow a first glimpse on the subtle regulatory mechanisms that drive the process of chalky and foliated layers deposition. |
author2 |
School of Natural Sciences Trinity College Dublin Institut des Sciences de la Terre de Paris (iSTeP) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'Ecogéochimie des environnements benthiques (LECOB) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Biogéosciences UMR 6282 Dijon (BGS) Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement Laboratoire SUBATECH Nantes (SUBATECH) Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Nantes (UN)-Mines Nantes (Mines Nantes) Laboratoire de biogenèse membranaire (LBM) Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) Géosciences Environnement Toulouse (GET) Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP) Météo France-Centre National d'Études Spatiales Toulouse (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD) Institut Cochin (IC UM3 (UMR 8104 / U1016)) Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Mouchi, Vincent Lartaud, Franck Guichard, Nathalie Immel, Françoise De Rafélis, Marc Broussard, Cédric Crowley, Quentin G. Marin, Frédéric |
author_facet |
Mouchi, Vincent Lartaud, Franck Guichard, Nathalie Immel, Françoise De Rafélis, Marc Broussard, Cédric Crowley, Quentin G. Marin, Frédéric |
author_sort |
Mouchi, Vincent |
title |
Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas |
title_short |
Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas |
title_full |
Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas |
title_fullStr |
Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas |
title_full_unstemmed |
Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas |
title_sort |
chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.sorbonne-universite.fr/hal-01400675 https://hal.sorbonne-universite.fr/hal-01400675/document https://hal.sorbonne-universite.fr/hal-01400675/file/Mouchi_2016_Chalky_versus.pdf https://doi.org/10.1007/s00227-016-3040-6 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0025-3162 EISSN: 1432-1793 Marine Biology https://hal.sorbonne-universite.fr/hal-01400675 Marine Biology, Springer Verlag, 2016, 163 (12), pp.256. ⟨10.1007/s00227-016-3040-6⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00227-016-3040-6 hal-01400675 https://hal.sorbonne-universite.fr/hal-01400675 https://hal.sorbonne-universite.fr/hal-01400675/document https://hal.sorbonne-universite.fr/hal-01400675/file/Mouchi_2016_Chalky_versus.pdf doi:10.1007/s00227-016-3040-6 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s00227-016-3040-6 |
container_title |
Marine Biology |
container_volume |
163 |
container_issue |
12 |
_version_ |
1766393918550704128 |
spelling |
ftccsdartic:oai:HAL:hal-01400675v1 2023-05-15T15:58:11+02:00 Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas Mouchi, Vincent Lartaud, Franck Guichard, Nathalie Immel, Françoise De Rafélis, Marc Broussard, Cédric Crowley, Quentin G. Marin, Frédéric School of Natural Sciences Trinity College Dublin Institut des Sciences de la Terre de Paris (iSTeP) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'Ecogéochimie des environnements benthiques (LECOB) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Biogéosciences UMR 6282 Dijon (BGS) Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement Laboratoire SUBATECH Nantes (SUBATECH) Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Nantes (UN)-Mines Nantes (Mines Nantes) Laboratoire de biogenèse membranaire (LBM) Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) Géosciences Environnement Toulouse (GET) Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP) Météo France-Centre National d'Études Spatiales Toulouse (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD) Institut Cochin (IC UM3 (UMR 8104 / U1016)) Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) 2016 https://hal.sorbonne-universite.fr/hal-01400675 https://hal.sorbonne-universite.fr/hal-01400675/document https://hal.sorbonne-universite.fr/hal-01400675/file/Mouchi_2016_Chalky_versus.pdf https://doi.org/10.1007/s00227-016-3040-6 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00227-016-3040-6 hal-01400675 https://hal.sorbonne-universite.fr/hal-01400675 https://hal.sorbonne-universite.fr/hal-01400675/document https://hal.sorbonne-universite.fr/hal-01400675/file/Mouchi_2016_Chalky_versus.pdf doi:10.1007/s00227-016-3040-6 info:eu-repo/semantics/OpenAccess ISSN: 0025-3162 EISSN: 1432-1793 Marine Biology https://hal.sorbonne-universite.fr/hal-01400675 Marine Biology, Springer Verlag, 2016, 163 (12), pp.256. ⟨10.1007/s00227-016-3040-6⟩ [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU]Sciences of the Universe [physics]/Earth Sciences info:eu-repo/semantics/article Journal articles 2016 ftccsdartic https://doi.org/10.1007/s00227-016-3040-6 2021-11-21T02:40:47Z International audience Mollusc shells are organic–inorganic biocomposites, arranged in a limited number of superimposed calcified layers that generally exhibit very different organization of their crystallites. Because of their attractive mechanical and crystallographic properties, these shell layers have been the focus of several physical and biochemical characterizations. In particular, recent proteomic data obtained from individual layers suggest that their protein contents are different. However, the direct visual evidence that some macromolecular components are layer-specific is rather tenuous. This paper is based on a non-conventional immunogold labelling approach to localize proteins in the shell of the edible oyster Crassostrea gigas. The shell microstructure of this model organism is predominantly composed of foliated calcite, interspersed by discontinuous pockets of ‘chalky layers’, a porous microstructure typical of bivalves of the ostreid family. By developing a polyclonal antibody (in two rats) elicited against a proteinaceous shell fraction, we obtained differential staining of the two microstructures. We assert that our labelling is microstructure discriminant. The difference in labelling of the two shell microstructures suggests either that they are formed by a variation of the secretory repertoire of the shell-forming cells of the calcifying mantle epithelium or that the chalky layer may be formed via a completely different mechanism. Our results allow a first glimpse on the subtle regulatory mechanisms that drive the process of chalky and foliated layers deposition. Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Marine Biology 163 12 |