Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
International audience Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of u...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2014
|
Subjects: | |
Online Access: | https://hal.sorbonne-universite.fr/hal-01358986 https://hal.sorbonne-universite.fr/hal-01358986/document https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf https://doi.org/10.1038/srep04661 |
id |
ftccsdartic:oai:HAL:hal-01358986v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:hal-01358986v1 2024-02-27T08:37:24+00:00 Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean Pedneault, Estelle Galand, Pierre E Potvin, Marianne Tremblay, Jean-Eric Lovejoy, Connie Université Laval Québec (ULaval) Laboratoire d'Ecogéochimie des environnements benthiques (LECOB) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2014-04-11 https://hal.sorbonne-universite.fr/hal-01358986 https://hal.sorbonne-universite.fr/hal-01358986/document https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf https://doi.org/10.1038/srep04661 en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/srep04661 hal-01358986 https://hal.sorbonne-universite.fr/hal-01358986 https://hal.sorbonne-universite.fr/hal-01358986/document https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf doi:10.1038/srep04661 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports https://hal.sorbonne-universite.fr/hal-01358986 Scientific Reports, 2014, 4, pp.4661. ⟨10.1038/srep04661⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2014 ftccsdartic https://doi.org/10.1038/srep04661 2024-01-28T03:48:45Z International audience Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean. Article in Journal/Newspaper Arctic Arctic Ocean Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic Arctic Ocean Scientific Reports 4 1 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Pedneault, Estelle Galand, Pierre E Potvin, Marianne Tremblay, Jean-Eric Lovejoy, Connie Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean. |
author2 |
Université Laval Québec (ULaval) Laboratoire d'Ecogéochimie des environnements benthiques (LECOB) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Pedneault, Estelle Galand, Pierre E Potvin, Marianne Tremblay, Jean-Eric Lovejoy, Connie |
author_facet |
Pedneault, Estelle Galand, Pierre E Potvin, Marianne Tremblay, Jean-Eric Lovejoy, Connie |
author_sort |
Pedneault, Estelle |
title |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_short |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_full |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_fullStr |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_full_unstemmed |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_sort |
archaeal amoa and urec genes and their transcriptional activity in the arctic ocean |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.sorbonne-universite.fr/hal-01358986 https://hal.sorbonne-universite.fr/hal-01358986/document https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf https://doi.org/10.1038/srep04661 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports https://hal.sorbonne-universite.fr/hal-01358986 Scientific Reports, 2014, 4, pp.4661. ⟨10.1038/srep04661⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/srep04661 hal-01358986 https://hal.sorbonne-universite.fr/hal-01358986 https://hal.sorbonne-universite.fr/hal-01358986/document https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf doi:10.1038/srep04661 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1038/srep04661 |
container_title |
Scientific Reports |
container_volume |
4 |
container_issue |
1 |
_version_ |
1792044409030705152 |