The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity
International audience BackgroundOne of the five basal actinopterygian lineages, the Chondrostei, including sturgeon, shovelnose, and paddlefish (Order Acipenseriformes) show extraordinary ploidy diversity associated with three rounds of lineage-specific whole-genome duplication, resulting in three...
Published in: | Genetics Selection Evolution |
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ftccsdartic:oai:HAL:hal-01341346v1 2023-05-15T13:01:57+02:00 The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity Havelka, Miloš Bytyutskyy, Dmytro Symonová, Radka Ráb, Petr Flajšhans, Martin 2016-12 https://hal.archives-ouvertes.fr/hal-01341346 https://hal.archives-ouvertes.fr/hal-01341346/document https://hal.archives-ouvertes.fr/hal-01341346/file/12711_2016_Article_194.pdf https://doi.org/10.1186/s12711-016-0194-0 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1186/s12711-016-0194-0 hal-01341346 https://hal.archives-ouvertes.fr/hal-01341346 https://hal.archives-ouvertes.fr/hal-01341346/document https://hal.archives-ouvertes.fr/hal-01341346/file/12711_2016_Article_194.pdf doi:10.1186/s12711-016-0194-0 info:eu-repo/semantics/OpenAccess EISSN: 1297-9686 Genetics Selection Evolution https://hal.archives-ouvertes.fr/hal-01341346 Genetics Selection Evolution, 2016, 48 (1), pp.12. ⟨10.1186/s12711-016-0194-0⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2016 ftccsdartic https://doi.org/10.1186/s12711-016-0194-0 2021-02-21T01:25:57Z International audience BackgroundOne of the five basal actinopterygian lineages, the Chondrostei, including sturgeon, shovelnose, and paddlefish (Order Acipenseriformes) show extraordinary ploidy diversity associated with three rounds of lineage-specific whole-genome duplication, resulting in three levels of ploidy in sturgeon. Recently, incidence of spontaneous polyploidization has been reported among cultured sturgeon and it could have serious negative implications for the economics of sturgeon farming. We report the occurrence of seven spontaneous heptaploid (7n) Siberian sturgeon Acipenser baerii, which is a functional tetraploid species (4n) with ~245 chromosomes. Our aims were to assess ploidy level and chromosome number of the analysed specimens and to identify the possible mechanism that underlies the occurrence of spontaneous additional chromosome sets in their genome.ResultsAmong 150 specimens resulting from the mating of a tetraploid (4n) A. baerii (~245 chromosomes) dam with a hexaploid (6n) A. baerii (~368 chromosomes) sire, 143 displayed a relative DNA content that corresponds to pentaploidy (5n) with an absolute DNA content of 8.98 ± 0.03 pg DNA per nucleus and nuclear area of 35.3 ± 4.3 μm2 and seven specimens exhibited a relative DNA content that corresponds to heptaploidy (7n), with an absolute DNA content of 15.02 ± 0.04 pg DNA per nucleus and nuclear area of 48.4 ± 5.1 μm2. Chromosome analyses confirmed a modal number of ~437 chromosomes in these heptaploid (7n) individuals. DNA genotyping of eight microsatellite loci followed by parental assignment confirmed spontaneous duplication of the maternal chromosome sets via retention of the second polar body in meiosis II as the mechanism for the formation of this unusual chromosome number and ploidy level in a functional tetraploid A. baerii.ConclusionsWe report the second highest chromosome count among vertebrates in cultured sturgeon (~437) after the schizothoracine cyprinid Ptychobarbus dipogon with ~446 chromosomes. The finding also ... Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Genetics Selection Evolution 48 1 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
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[SDV]Life Sciences [q-bio] Havelka, Miloš Bytyutskyy, Dmytro Symonová, Radka Ráb, Petr Flajšhans, Martin The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
International audience BackgroundOne of the five basal actinopterygian lineages, the Chondrostei, including sturgeon, shovelnose, and paddlefish (Order Acipenseriformes) show extraordinary ploidy diversity associated with three rounds of lineage-specific whole-genome duplication, resulting in three levels of ploidy in sturgeon. Recently, incidence of spontaneous polyploidization has been reported among cultured sturgeon and it could have serious negative implications for the economics of sturgeon farming. We report the occurrence of seven spontaneous heptaploid (7n) Siberian sturgeon Acipenser baerii, which is a functional tetraploid species (4n) with ~245 chromosomes. Our aims were to assess ploidy level and chromosome number of the analysed specimens and to identify the possible mechanism that underlies the occurrence of spontaneous additional chromosome sets in their genome.ResultsAmong 150 specimens resulting from the mating of a tetraploid (4n) A. baerii (~245 chromosomes) dam with a hexaploid (6n) A. baerii (~368 chromosomes) sire, 143 displayed a relative DNA content that corresponds to pentaploidy (5n) with an absolute DNA content of 8.98 ± 0.03 pg DNA per nucleus and nuclear area of 35.3 ± 4.3 μm2 and seven specimens exhibited a relative DNA content that corresponds to heptaploidy (7n), with an absolute DNA content of 15.02 ± 0.04 pg DNA per nucleus and nuclear area of 48.4 ± 5.1 μm2. Chromosome analyses confirmed a modal number of ~437 chromosomes in these heptaploid (7n) individuals. DNA genotyping of eight microsatellite loci followed by parental assignment confirmed spontaneous duplication of the maternal chromosome sets via retention of the second polar body in meiosis II as the mechanism for the formation of this unusual chromosome number and ploidy level in a functional tetraploid A. baerii.ConclusionsWe report the second highest chromosome count among vertebrates in cultured sturgeon (~437) after the schizothoracine cyprinid Ptychobarbus dipogon with ~446 chromosomes. The finding also ... |
format |
Article in Journal/Newspaper |
author |
Havelka, Miloš Bytyutskyy, Dmytro Symonová, Radka Ráb, Petr Flajšhans, Martin |
author_facet |
Havelka, Miloš Bytyutskyy, Dmytro Symonová, Radka Ráb, Petr Flajšhans, Martin |
author_sort |
Havelka, Miloš |
title |
The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity |
title_short |
The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity |
title_full |
The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity |
title_fullStr |
The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity |
title_full_unstemmed |
The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity |
title_sort |
second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.archives-ouvertes.fr/hal-01341346 https://hal.archives-ouvertes.fr/hal-01341346/document https://hal.archives-ouvertes.fr/hal-01341346/file/12711_2016_Article_194.pdf https://doi.org/10.1186/s12711-016-0194-0 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
EISSN: 1297-9686 Genetics Selection Evolution https://hal.archives-ouvertes.fr/hal-01341346 Genetics Selection Evolution, 2016, 48 (1), pp.12. ⟨10.1186/s12711-016-0194-0⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12711-016-0194-0 hal-01341346 https://hal.archives-ouvertes.fr/hal-01341346 https://hal.archives-ouvertes.fr/hal-01341346/document https://hal.archives-ouvertes.fr/hal-01341346/file/12711_2016_Article_194.pdf doi:10.1186/s12711-016-0194-0 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s12711-016-0194-0 |
container_title |
Genetics Selection Evolution |
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48 |
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1 |
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1766290967270260736 |