Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment
Silicate weathering improves soils by releasing bioessential nutrients from the bedrock to the soil ecosystem. However, whether bacteria are capable of inhabiting subsurface critical zones (zone of active rock weathering), and their role therein, are unknown. Next-generation sequencing and community...
Published in: | FEMS Microbiology Ecology |
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Online Access: | https://hal.archives-ouvertes.fr/hal-01268513 https://doi.org/10.1111/1574-6941.12167 |
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ftccsdartic:oai:HAL:hal-01268513v1 2023-05-15T15:09:02+02:00 Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment Summers, Stephen Whiteley, Andrew S. Kelly, Laura Cockell, Charles S. Centre for Ecology and Hydrology SUPA School of Physics and Astronomy Edinburgh University of Edinburgh School of Earth and Environment The University of Western Australia (UWA) Interactions Arbres-Microorganismes (IAM) Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) NERC Centre for Ecology and Hydrology, UK; Open University, UK 2013 https://hal.archives-ouvertes.fr/hal-01268513 https://doi.org/10.1111/1574-6941.12167 en eng HAL CCSD Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/1574-6941.12167 info:eu-repo/semantics/altIdentifier/pmid/23777316 hal-01268513 https://hal.archives-ouvertes.fr/hal-01268513 doi:10.1111/1574-6941.12167 PRODINRA: 257559 PUBMED: 23777316 WOS: 000326960000002 ISSN: 0168-6496 EISSN: 1574-6941 FEMS Microbiology Ecology https://hal.archives-ouvertes.fr/hal-01268513 FEMS Microbiology Ecology, Wiley-Blackwell, 2013, 86 (3), pp.381-393. ⟨10.1111/1574-6941.12167⟩ weathering critical zone bacteria minerals molecular ecology soil microbiology [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.1111/1574-6941.12167 2021-11-28T01:12:12Z Silicate weathering improves soils by releasing bioessential nutrients from the bedrock to the soil ecosystem. However, whether bacteria are capable of inhabiting subsurface critical zones (zone of active rock weathering), and their role therein, are unknown. Next-generation sequencing and community fingerprinting permitted us to characterize communities from an Icelandic critical zone environment. Communities were compared with respect to physico-chemical properties of the environment to determine the factors influencing bacterial diversity. We showed that land coverage influenced critical zone communities. Analysis of tree-covered site (TCS) soils exhibited high cell densities (TCS=2.25x 10(7)g(-1)), whereas lichen- and moss-covered sites (LMS) had lower cell densities (LMS=1.06x10(7)cellsg(-1)), thought to be a result of the organic carbon produced by the trees. Differences in the bacterial community were observed from the abundance of 16S rRNA gene sequences affiliated with Acidobacteria and Proteobacteria, with TCS possessing higher abundances of Proteobacteria [no of sequences: LMS=1526 (+/- 497); TCS=2214 (+/- 531)], specifically Alpha- and Betaproteobacteria, and lower Acidobacteria numbers [no of sequences: LMS=1244 (+/- 338); TCS=598 (+/- 140)]. Diversity indices and 16S rRNA gene rarefaction showed that communities from TCS soils had lower -diversity than sites without, indicative of specialized communities at sites with root-forming plants. Article in Journal/Newspaper Arctic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic FEMS Microbiology Ecology 86 3 381 393 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
weathering critical zone bacteria minerals molecular ecology soil microbiology [SDV]Life Sciences [q-bio] |
spellingShingle |
weathering critical zone bacteria minerals molecular ecology soil microbiology [SDV]Life Sciences [q-bio] Summers, Stephen Whiteley, Andrew S. Kelly, Laura Cockell, Charles S. Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
topic_facet |
weathering critical zone bacteria minerals molecular ecology soil microbiology [SDV]Life Sciences [q-bio] |
description |
Silicate weathering improves soils by releasing bioessential nutrients from the bedrock to the soil ecosystem. However, whether bacteria are capable of inhabiting subsurface critical zones (zone of active rock weathering), and their role therein, are unknown. Next-generation sequencing and community fingerprinting permitted us to characterize communities from an Icelandic critical zone environment. Communities were compared with respect to physico-chemical properties of the environment to determine the factors influencing bacterial diversity. We showed that land coverage influenced critical zone communities. Analysis of tree-covered site (TCS) soils exhibited high cell densities (TCS=2.25x 10(7)g(-1)), whereas lichen- and moss-covered sites (LMS) had lower cell densities (LMS=1.06x10(7)cellsg(-1)), thought to be a result of the organic carbon produced by the trees. Differences in the bacterial community were observed from the abundance of 16S rRNA gene sequences affiliated with Acidobacteria and Proteobacteria, with TCS possessing higher abundances of Proteobacteria [no of sequences: LMS=1526 (+/- 497); TCS=2214 (+/- 531)], specifically Alpha- and Betaproteobacteria, and lower Acidobacteria numbers [no of sequences: LMS=1244 (+/- 338); TCS=598 (+/- 140)]. Diversity indices and 16S rRNA gene rarefaction showed that communities from TCS soils had lower -diversity than sites without, indicative of specialized communities at sites with root-forming plants. |
author2 |
Centre for Ecology and Hydrology SUPA School of Physics and Astronomy Edinburgh University of Edinburgh School of Earth and Environment The University of Western Australia (UWA) Interactions Arbres-Microorganismes (IAM) Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) NERC Centre for Ecology and Hydrology, UK; Open University, UK |
format |
Article in Journal/Newspaper |
author |
Summers, Stephen Whiteley, Andrew S. Kelly, Laura Cockell, Charles S. |
author_facet |
Summers, Stephen Whiteley, Andrew S. Kelly, Laura Cockell, Charles S. |
author_sort |
Summers, Stephen |
title |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_short |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_full |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_fullStr |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_full_unstemmed |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_sort |
land coverage influences the bacterial community composition in the critical zone of a sub-arctic basaltic environment |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.archives-ouvertes.fr/hal-01268513 https://doi.org/10.1111/1574-6941.12167 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
ISSN: 0168-6496 EISSN: 1574-6941 FEMS Microbiology Ecology https://hal.archives-ouvertes.fr/hal-01268513 FEMS Microbiology Ecology, Wiley-Blackwell, 2013, 86 (3), pp.381-393. ⟨10.1111/1574-6941.12167⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/1574-6941.12167 info:eu-repo/semantics/altIdentifier/pmid/23777316 hal-01268513 https://hal.archives-ouvertes.fr/hal-01268513 doi:10.1111/1574-6941.12167 PRODINRA: 257559 PUBMED: 23777316 WOS: 000326960000002 |
op_doi |
https://doi.org/10.1111/1574-6941.12167 |
container_title |
FEMS Microbiology Ecology |
container_volume |
86 |
container_issue |
3 |
container_start_page |
381 |
op_container_end_page |
393 |
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1766340279040737280 |