OsHV-1 countermeasures to the Pacific oyster's anti-viral response
International audience The hostepathogen interactions between the Pacific oyster (Crassostrea gigas) and Ostreid herpesvirus type 1 (OsHV-1) are poorly characterised. Herpesviruses are a group of large, DNA viruses that are known to encode gene products that subvert their host's antiviral respo...
Published in: | Fish & Shellfish Immunology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hal.archives-ouvertes.fr/hal-01260118 https://hal.archives-ouvertes.fr/hal-01260118/document https://hal.archives-ouvertes.fr/hal-01260118/file/Green-2015-FishShelfImmunol-OSHV-1.pdf https://doi.org/10.1016/j.fsi.2015.09.025 |
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ftccsdartic:oai:HAL:hal-01260118v1 2023-05-15T15:59:04+02:00 OsHV-1 countermeasures to the Pacific oyster's anti-viral response Green, Timothy Rolland, Jean-Luc Vergnes, Agnès Raftos, David Montagnani, Caroline University of Sydney Institute of Marine Science (USIMS) The University of Sydney Dpt Biological Sciences, Macquarie University Macquarie University Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) 2015 https://hal.archives-ouvertes.fr/hal-01260118 https://hal.archives-ouvertes.fr/hal-01260118/document https://hal.archives-ouvertes.fr/hal-01260118/file/Green-2015-FishShelfImmunol-OSHV-1.pdf https://doi.org/10.1016/j.fsi.2015.09.025 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2015.09.025 hal-01260118 https://hal.archives-ouvertes.fr/hal-01260118 https://hal.archives-ouvertes.fr/hal-01260118/document https://hal.archives-ouvertes.fr/hal-01260118/file/Green-2015-FishShelfImmunol-OSHV-1.pdf doi:10.1016/j.fsi.2015.09.025 info:eu-repo/semantics/OpenAccess ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.archives-ouvertes.fr/hal-01260118 Fish and Shellfish Immunology, Elsevier, 2015, 47 (1), pp.435-443. ⟨10.1016/j.fsi.2015.09.025⟩ Anti-viral response Apoptosis Crassostrea Interferon-like Poly I:C [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] info:eu-repo/semantics/article Journal articles 2015 ftccsdartic https://doi.org/10.1016/j.fsi.2015.09.025 2021-10-24T00:06:04Z International audience The hostepathogen interactions between the Pacific oyster (Crassostrea gigas) and Ostreid herpesvirus type 1 (OsHV-1) are poorly characterised. Herpesviruses are a group of large, DNA viruses that are known to encode gene products that subvert their host's antiviral response. It is likely that OsHV-1 has also evolved similar strategies as its genome encodes genes with high homology to C. gigas inhibitors of apoptosis (IAPs) and an interferon-stimulated gene (termed CH25H). The first objective of this study was to simultaneously investigate the expression of C. gigas and OsHV-1 genes that share high sequence homology during an acute infection. Comparison of apoptosis-related genes revealed that components of the extrinsic apoptosis pathway (TNF) were induced in response to OsHV-1 infection, but we failed to observe evidence of apoptosis using a combination of biochemical and molecular assays. IAPs encoded by OsHV-1 were highly expressed during the acute stage of infection and may explain why we didn't observe evidence of apoptosis. However, C. gigas must have an alternative mechanism to apoptosis for clearing OsHV-1 from infected gill cells as we observed a reduction in viral DNA between 27 and 54 h post-infection. The reduction of viral DNA in C. gigas gill cells occurred after the up-regulation of interferon-stimulated genes (viperin, PKR, ADAR). In a second objective, we manipulated the host's antiviral response by injecting C. gigas with a small dose of poly I:C at the time of OsHV-1 infection. This small dose of poly I:C was unable to induce transcription of known antiviral effectors (ISGs), but these oysters were still capable of inhibiting OsHV-1 replication. This result suggests dsRNA induces an antiviral response that is additional to the IFN-like pathway Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Fish & Shellfish Immunology 47 1 435 443 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Anti-viral response Apoptosis Crassostrea Interferon-like Poly I:C [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] |
spellingShingle |
Anti-viral response Apoptosis Crassostrea Interferon-like Poly I:C [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] Green, Timothy Rolland, Jean-Luc Vergnes, Agnès Raftos, David Montagnani, Caroline OsHV-1 countermeasures to the Pacific oyster's anti-viral response |
topic_facet |
Anti-viral response Apoptosis Crassostrea Interferon-like Poly I:C [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] |
description |
International audience The hostepathogen interactions between the Pacific oyster (Crassostrea gigas) and Ostreid herpesvirus type 1 (OsHV-1) are poorly characterised. Herpesviruses are a group of large, DNA viruses that are known to encode gene products that subvert their host's antiviral response. It is likely that OsHV-1 has also evolved similar strategies as its genome encodes genes with high homology to C. gigas inhibitors of apoptosis (IAPs) and an interferon-stimulated gene (termed CH25H). The first objective of this study was to simultaneously investigate the expression of C. gigas and OsHV-1 genes that share high sequence homology during an acute infection. Comparison of apoptosis-related genes revealed that components of the extrinsic apoptosis pathway (TNF) were induced in response to OsHV-1 infection, but we failed to observe evidence of apoptosis using a combination of biochemical and molecular assays. IAPs encoded by OsHV-1 were highly expressed during the acute stage of infection and may explain why we didn't observe evidence of apoptosis. However, C. gigas must have an alternative mechanism to apoptosis for clearing OsHV-1 from infected gill cells as we observed a reduction in viral DNA between 27 and 54 h post-infection. The reduction of viral DNA in C. gigas gill cells occurred after the up-regulation of interferon-stimulated genes (viperin, PKR, ADAR). In a second objective, we manipulated the host's antiviral response by injecting C. gigas with a small dose of poly I:C at the time of OsHV-1 infection. This small dose of poly I:C was unable to induce transcription of known antiviral effectors (ISGs), but these oysters were still capable of inhibiting OsHV-1 replication. This result suggests dsRNA induces an antiviral response that is additional to the IFN-like pathway |
author2 |
University of Sydney Institute of Marine Science (USIMS) The University of Sydney Dpt Biological Sciences, Macquarie University Macquarie University Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) |
format |
Article in Journal/Newspaper |
author |
Green, Timothy Rolland, Jean-Luc Vergnes, Agnès Raftos, David Montagnani, Caroline |
author_facet |
Green, Timothy Rolland, Jean-Luc Vergnes, Agnès Raftos, David Montagnani, Caroline |
author_sort |
Green, Timothy |
title |
OsHV-1 countermeasures to the Pacific oyster's anti-viral response |
title_short |
OsHV-1 countermeasures to the Pacific oyster's anti-viral response |
title_full |
OsHV-1 countermeasures to the Pacific oyster's anti-viral response |
title_fullStr |
OsHV-1 countermeasures to the Pacific oyster's anti-viral response |
title_full_unstemmed |
OsHV-1 countermeasures to the Pacific oyster's anti-viral response |
title_sort |
oshv-1 countermeasures to the pacific oyster's anti-viral response |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.archives-ouvertes.fr/hal-01260118 https://hal.archives-ouvertes.fr/hal-01260118/document https://hal.archives-ouvertes.fr/hal-01260118/file/Green-2015-FishShelfImmunol-OSHV-1.pdf https://doi.org/10.1016/j.fsi.2015.09.025 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.archives-ouvertes.fr/hal-01260118 Fish and Shellfish Immunology, Elsevier, 2015, 47 (1), pp.435-443. ⟨10.1016/j.fsi.2015.09.025⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2015.09.025 hal-01260118 https://hal.archives-ouvertes.fr/hal-01260118 https://hal.archives-ouvertes.fr/hal-01260118/document https://hal.archives-ouvertes.fr/hal-01260118/file/Green-2015-FishShelfImmunol-OSHV-1.pdf doi:10.1016/j.fsi.2015.09.025 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2015.09.025 |
container_title |
Fish & Shellfish Immunology |
container_volume |
47 |
container_issue |
1 |
container_start_page |
435 |
op_container_end_page |
443 |
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