Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species
International audience A systematic search for viral infection was performed in the isolated Kerguelen Islands, using a range of polyvalent genus-specific PCR assays. Barley yellow dwarf virus (BYDV) was detected in both introduced and native grasses such as Poa cookii. The geographical distribution...
Published in: | PLoS ONE |
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Main Authors: | , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2013
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Online Access: | https://hal.archives-ouvertes.fr/hal-01208609 https://hal.archives-ouvertes.fr/hal-01208609/document https://hal.archives-ouvertes.fr/hal-01208609/file/2013_Svanella-Dumas_Plos%20One_1.pdf https://doi.org/10.1371/journal.pone.0067231 |
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English |
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[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Svanella-Dumas, Laurence Candresse, Thierry, Hullé, Maurice Marais-Colombel, Armelle Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
topic_facet |
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] |
description |
International audience A systematic search for viral infection was performed in the isolated Kerguelen Islands, using a range of polyvalent genus-specific PCR assays. Barley yellow dwarf virus (BYDV) was detected in both introduced and native grasses such as Poa cookii. The geographical distribution of BYDV and its prevalence in P. cookii were analyzed using samples collected from various sites of the archipelago. We estimate the average prevalence of BYDV to be 24.9% in P. cookii, with significant variability between sites. BYDV genetic diversity was assessed using sequence information from two genomic regions: the P3 open reading frame (ORF) (encoding the coat protein) and the hypervariable P6 ORF region. The phylogenetic analysis in the P3 region showed that BYDV sequences segregate into three major lineages, the most frequent of which (Ker-I cluster) showed close homology with BYDV-PAV-I isolates and had very low intra-lineage diversity (0.6%). A similarly low diversity was also recorded in the hypervariable P6 region, suggesting that Ker-I isolates derive from the recent introduction of BYDV-PAV-I. Divergence time estimation suggests that BYDV-PAV-I was likely introduced in the Kerguelen environment at the same time frame as its aphid vector, Rhopalosiphum padi, whose distribution shows good overlap with that of BYDV-Ker-I. The two other lineages show more than 22% amino acid divergence in the P3 region with other known species in the BYDV species complex, indicating that they represent distinct BYDV species. Using species-specific amplification primers, the distribution of these novel species was analyzed. The high prevalence of BYDV on native Poaceae and the presence of the vector R. padi, raises the question of its impact on the vulnerable plant communities of this remote ecosystem. |
author2 |
Biologie du fruit et pathologie (BFP) Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 Institut de Génétique, Environnement et Protection des Plantes (IGEPP) Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
format |
Article in Journal/Newspaper |
author |
Svanella-Dumas, Laurence Candresse, Thierry, Hullé, Maurice Marais-Colombel, Armelle |
author_facet |
Svanella-Dumas, Laurence Candresse, Thierry, Hullé, Maurice Marais-Colombel, Armelle |
author_sort |
Svanella-Dumas, Laurence |
title |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_short |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_full |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_fullStr |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_full_unstemmed |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_sort |
distribution of barley yellow dwarf virus-pav in the sub-antarctic kerguelen islands and characterization of two new [i]luteovirus[/i] species |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.archives-ouvertes.fr/hal-01208609 https://hal.archives-ouvertes.fr/hal-01208609/document https://hal.archives-ouvertes.fr/hal-01208609/file/2013_Svanella-Dumas_Plos%20One_1.pdf https://doi.org/10.1371/journal.pone.0067231 |
geographic |
Antarctic Kerguelen Kerguelen Islands |
geographic_facet |
Antarctic Kerguelen Kerguelen Islands |
genre |
Antarc* Antarctic Kerguelen Islands |
genre_facet |
Antarc* Antarctic Kerguelen Islands |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.archives-ouvertes.fr/hal-01208609 PLoS ONE, Public Library of Science, 2013, 8 (6), pp.e67231. ⟨10.1371/journal.pone.0067231⟩ http://www.plosone.org/ |
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op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0067231 |
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PLoS ONE |
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container_start_page |
e67231 |
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ftccsdartic:oai:HAL:hal-01208609v1 2023-05-15T13:49:54+02:00 Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species Svanella-Dumas, Laurence Candresse, Thierry, Hullé, Maurice Marais-Colombel, Armelle Biologie du fruit et pathologie (BFP) Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 Institut de Génétique, Environnement et Protection des Plantes (IGEPP) Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) 2013 https://hal.archives-ouvertes.fr/hal-01208609 https://hal.archives-ouvertes.fr/hal-01208609/document https://hal.archives-ouvertes.fr/hal-01208609/file/2013_Svanella-Dumas_Plos%20One_1.pdf https://doi.org/10.1371/journal.pone.0067231 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/pmid/23825645 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0067231 hal-01208609 https://hal.archives-ouvertes.fr/hal-01208609 https://hal.archives-ouvertes.fr/hal-01208609/document https://hal.archives-ouvertes.fr/hal-01208609/file/2013_Svanella-Dumas_Plos%20One_1.pdf PUBMED: 23825645 doi:10.1371/journal.pone.0067231 PRODINRA: 194274 WOS: 000320576400180 info:eu-repo/semantics/OpenAccess ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.archives-ouvertes.fr/hal-01208609 PLoS ONE, Public Library of Science, 2013, 8 (6), pp.e67231. ⟨10.1371/journal.pone.0067231⟩ http://www.plosone.org/ [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.1371/journal.pone.0067231 2021-10-24T01:19:39Z International audience A systematic search for viral infection was performed in the isolated Kerguelen Islands, using a range of polyvalent genus-specific PCR assays. Barley yellow dwarf virus (BYDV) was detected in both introduced and native grasses such as Poa cookii. The geographical distribution of BYDV and its prevalence in P. cookii were analyzed using samples collected from various sites of the archipelago. We estimate the average prevalence of BYDV to be 24.9% in P. cookii, with significant variability between sites. BYDV genetic diversity was assessed using sequence information from two genomic regions: the P3 open reading frame (ORF) (encoding the coat protein) and the hypervariable P6 ORF region. The phylogenetic analysis in the P3 region showed that BYDV sequences segregate into three major lineages, the most frequent of which (Ker-I cluster) showed close homology with BYDV-PAV-I isolates and had very low intra-lineage diversity (0.6%). A similarly low diversity was also recorded in the hypervariable P6 region, suggesting that Ker-I isolates derive from the recent introduction of BYDV-PAV-I. Divergence time estimation suggests that BYDV-PAV-I was likely introduced in the Kerguelen environment at the same time frame as its aphid vector, Rhopalosiphum padi, whose distribution shows good overlap with that of BYDV-Ker-I. The two other lineages show more than 22% amino acid divergence in the P3 region with other known species in the BYDV species complex, indicating that they represent distinct BYDV species. Using species-specific amplification primers, the distribution of these novel species was analyzed. The high prevalence of BYDV on native Poaceae and the presence of the vector R. padi, raises the question of its impact on the vulnerable plant communities of this remote ecosystem. Article in Journal/Newspaper Antarc* Antarctic Kerguelen Islands Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Antarctic Kerguelen Kerguelen Islands PLoS ONE 8 6 e67231 |