Fe and C co-limitation of heterotrophic bacteria in the naturally fertilized region off the Kerguelen Islands
International audience It has been univocally shown that iron (Fe) is the primary limiting nutrient for phytoplankton metabolism in high-nutrient, low-chlorophyll (HNLC) waters, yet the question of how this trace metal affects heterotrophic microbial activity is far less understood. We investigated...
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ftccsdartic:oai:HAL:hal-01207645v1 2023-05-15T17:02:02+02:00 Fe and C co-limitation of heterotrophic bacteria in the naturally fertilized region off the Kerguelen Islands Obernosterer, Ingrid Fourquez, M. Blain, S. Laboratoire d'Océanographie Microbienne (LOMIC) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2015 https://hal.sorbonne-universite.fr/hal-01207645 https://hal.sorbonne-universite.fr/hal-01207645/document https://hal.sorbonne-universite.fr/hal-01207645/file/bg-12-1983-2015.pdf https://doi.org/10.5194/bg-12-1983-2015 en eng HAL CCSD European Geosciences Union info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-12-1983-2015 hal-01207645 https://hal.sorbonne-universite.fr/hal-01207645 https://hal.sorbonne-universite.fr/hal-01207645/document https://hal.sorbonne-universite.fr/hal-01207645/file/bg-12-1983-2015.pdf doi:10.5194/bg-12-1983-2015 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1726-4170 EISSN: 1726-4189 Biogeosciences https://hal.sorbonne-universite.fr/hal-01207645 Biogeosciences, 2015, 12 (6), pp.1983-1992. ⟨10.5194/bg-12-1983-2015⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2015 ftccsdartic https://doi.org/10.5194/bg-12-1983-2015 2023-03-27T12:18:06Z International audience It has been univocally shown that iron (Fe) is the primary limiting nutrient for phytoplankton metabolism in high-nutrient, low-chlorophyll (HNLC) waters, yet the question of how this trace metal affects heterotrophic microbial activity is far less understood. We investigated the role of Fe for bacterial heterotrophic production and growth at three contrasting sites in the naturally Fe-fertilized region east of the Kerguelen Islands and at one site in HNLC waters during the KEOPS2 (Kerguelen Ocean and Plateau Compared Study 2) cruise in spring 2011. We performed dark incubations of natural microbial communities amended either with iron (Fe, as FeCl3) or carbon (C, as trace-metal clean glucose), or a combination of both, and followed bacterial abundance and heterotrophic production for up to 7 days. Our results show that single and combined additions of Fe and C stimulated bulk and cell-specific bacterial production at the Fe-fertilized sites, while in HNLC waters only combined additions resulted in significant increases in these parameters. Bacterial abundance was enhanced in two out of the three experiments performed in Fe-fertilized waters but did not respond to Fe or C additions in HNLC waters. Our results provide evidence that both Fe and C are present at limiting concentrations for bacterial heterotrophic activity in the naturally fertilized region off the Kerguelen Islands in spring, while bacteria were co-limited by these elements in HNLC waters. These results shed new light on the role of Fe in bacterial heterotrophic metabolism in regions of the Southern Ocean that receive variable Fe inputs. Article in Journal/Newspaper Kerguelen Islands Southern Ocean Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Southern Ocean Kerguelen Kerguelen Islands Biogeosciences 12 6 1983 1992 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Obernosterer, Ingrid Fourquez, M. Blain, S. Fe and C co-limitation of heterotrophic bacteria in the naturally fertilized region off the Kerguelen Islands |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience It has been univocally shown that iron (Fe) is the primary limiting nutrient for phytoplankton metabolism in high-nutrient, low-chlorophyll (HNLC) waters, yet the question of how this trace metal affects heterotrophic microbial activity is far less understood. We investigated the role of Fe for bacterial heterotrophic production and growth at three contrasting sites in the naturally Fe-fertilized region east of the Kerguelen Islands and at one site in HNLC waters during the KEOPS2 (Kerguelen Ocean and Plateau Compared Study 2) cruise in spring 2011. We performed dark incubations of natural microbial communities amended either with iron (Fe, as FeCl3) or carbon (C, as trace-metal clean glucose), or a combination of both, and followed bacterial abundance and heterotrophic production for up to 7 days. Our results show that single and combined additions of Fe and C stimulated bulk and cell-specific bacterial production at the Fe-fertilized sites, while in HNLC waters only combined additions resulted in significant increases in these parameters. Bacterial abundance was enhanced in two out of the three experiments performed in Fe-fertilized waters but did not respond to Fe or C additions in HNLC waters. Our results provide evidence that both Fe and C are present at limiting concentrations for bacterial heterotrophic activity in the naturally fertilized region off the Kerguelen Islands in spring, while bacteria were co-limited by these elements in HNLC waters. These results shed new light on the role of Fe in bacterial heterotrophic metabolism in regions of the Southern Ocean that receive variable Fe inputs. |
author2 |
Laboratoire d'Océanographie Microbienne (LOMIC) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Obernosterer, Ingrid Fourquez, M. Blain, S. |
author_facet |
Obernosterer, Ingrid Fourquez, M. Blain, S. |
author_sort |
Obernosterer, Ingrid |
title |
Fe and C co-limitation of heterotrophic bacteria in the naturally fertilized region off the Kerguelen Islands |
title_short |
Fe and C co-limitation of heterotrophic bacteria in the naturally fertilized region off the Kerguelen Islands |
title_full |
Fe and C co-limitation of heterotrophic bacteria in the naturally fertilized region off the Kerguelen Islands |
title_fullStr |
Fe and C co-limitation of heterotrophic bacteria in the naturally fertilized region off the Kerguelen Islands |
title_full_unstemmed |
Fe and C co-limitation of heterotrophic bacteria in the naturally fertilized region off the Kerguelen Islands |
title_sort |
fe and c co-limitation of heterotrophic bacteria in the naturally fertilized region off the kerguelen islands |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.sorbonne-universite.fr/hal-01207645 https://hal.sorbonne-universite.fr/hal-01207645/document https://hal.sorbonne-universite.fr/hal-01207645/file/bg-12-1983-2015.pdf https://doi.org/10.5194/bg-12-1983-2015 |
geographic |
Southern Ocean Kerguelen Kerguelen Islands |
geographic_facet |
Southern Ocean Kerguelen Kerguelen Islands |
genre |
Kerguelen Islands Southern Ocean |
genre_facet |
Kerguelen Islands Southern Ocean |
op_source |
ISSN: 1726-4170 EISSN: 1726-4189 Biogeosciences https://hal.sorbonne-universite.fr/hal-01207645 Biogeosciences, 2015, 12 (6), pp.1983-1992. ⟨10.5194/bg-12-1983-2015⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-12-1983-2015 hal-01207645 https://hal.sorbonne-universite.fr/hal-01207645 https://hal.sorbonne-universite.fr/hal-01207645/document https://hal.sorbonne-universite.fr/hal-01207645/file/bg-12-1983-2015.pdf doi:10.5194/bg-12-1983-2015 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.5194/bg-12-1983-2015 |
container_title |
Biogeosciences |
container_volume |
12 |
container_issue |
6 |
container_start_page |
1983 |
op_container_end_page |
1992 |
_version_ |
1766055258780336128 |