Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the Black-Lip Pearl oyster, Pinctada margaritifera
The present chapter aims at describing the main biological characteristics of two oyster species, both important for their commercial values. Oyster spermatozoa belong to the "aquasperm" according to ultra-structural data: they possess an acrosome and four mitochondria. The sperm flagellum...
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ftccsdartic:oai:HAL:hal-01205080v1 2023-05-15T15:59:03+02:00 Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the Black-Lip Pearl oyster, Pinctada margaritifera Demoy-Schneider, Marina Schmitt, Nelly Suquet, Marc Labbé, Catherine Boulais, Myrina Prokopchuk, Galina Cosson, Jacky University of French Polynesia UMR PFOM Department, Station Expérimental d'Argenton Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Physiologie et Génomique des Poissons (LPGP) Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) UMR PFOM Department, Station Expérimentale d'Argenton Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology University of South Bohemia CENAQUA (registration number CZ.1.05/2.1.00/01.0024. of Czech Republic) - Czech Agencies Projects: IAA 608030801, ME10015, QH82119, LC06073, CZ.1.05/2.1.00/01.0024, GACR P502/11/0090, GAJU 046/2010/Z Brenda T. Erickson 2014 https://hal.archives-ouvertes.fr/hal-01205080 en eng HAL CCSD Nova Science Publishers ISBN: 978-1-63117-020-1 hal-01205080 https://hal.archives-ouvertes.fr/hal-01205080 PRODINRA: 254710 Spermatozoa https://hal.archives-ouvertes.fr/hal-01205080 Spermatozoa, Nova Science Publishers, 259 p., 2014, 978-1-63117-020-1 https://www.novapublishers.com/catalog/product_info.php?products_id=47602 [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/bookPart Book sections 2014 ftccsdartic 2021-12-12T04:47:56Z The present chapter aims at describing the main biological characteristics of two oyster species, both important for their commercial values. Oyster spermatozoa belong to the "aquasperm" according to ultra-structural data: they possess an acrosome and four mitochondria. The sperm flagellum is devoid of progressive motility eitheir in the gonads or at spawning in sea water. In both cases, flagellar motility (% of moving cells) appears slowly in a time dependent manner in natural sea water (5 to 15 min). This ability to acquire potentiality for motility (maturation process) can be accelerated by sperm incubation with drugs like serotonine, theophilline, caffeine or dibutyryl-cAMP, which rapidly initiate progressive movement of live sperm. All drugs are effective in a time dependent manner and especially caffeine and theophilline are shown to act in a concentration dependent manner. These drugs allow forwardly directed translation for the majority of sperm cells. Furtheremore, spermatozoa induced to such efficient swimming behavior exhibit fertilizing performances of high levelDuring hatchery practices, oyster sperm are frequently collected by scarification of the male gonad alternatively, ejaculation can be induced by various methods. According to our results, injection of compounds such as serotonine (1 to 10mM) in the male gonad leads to release of sperm in sea water. By dilution into regular sea water, live oyster spermatozoa obtained by scarification show initially mostly erratic movements with poor efficiency for forward translation and initiate efficient flagellar motility after several minutes.Motility of black-lip pearl oyster spermatozoa appears gradually when spermatozoa are transferred into alkaline sea water. This motility-activating effect is reversed when pH is shifted back to acidic values; as in normal sea water, activity of sperm (% motile cells) increases gradually after alkaline pH activation and lasts for several minutes. The characteristics of these fully motile spermatozoa are described in ... Book Part Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
[SDV.BA]Life Sciences [q-bio]/Animal biology Demoy-Schneider, Marina Schmitt, Nelly Suquet, Marc Labbé, Catherine Boulais, Myrina Prokopchuk, Galina Cosson, Jacky Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the Black-Lip Pearl oyster, Pinctada margaritifera |
topic_facet |
[SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
The present chapter aims at describing the main biological characteristics of two oyster species, both important for their commercial values. Oyster spermatozoa belong to the "aquasperm" according to ultra-structural data: they possess an acrosome and four mitochondria. The sperm flagellum is devoid of progressive motility eitheir in the gonads or at spawning in sea water. In both cases, flagellar motility (% of moving cells) appears slowly in a time dependent manner in natural sea water (5 to 15 min). This ability to acquire potentiality for motility (maturation process) can be accelerated by sperm incubation with drugs like serotonine, theophilline, caffeine or dibutyryl-cAMP, which rapidly initiate progressive movement of live sperm. All drugs are effective in a time dependent manner and especially caffeine and theophilline are shown to act in a concentration dependent manner. These drugs allow forwardly directed translation for the majority of sperm cells. Furtheremore, spermatozoa induced to such efficient swimming behavior exhibit fertilizing performances of high levelDuring hatchery practices, oyster sperm are frequently collected by scarification of the male gonad alternatively, ejaculation can be induced by various methods. According to our results, injection of compounds such as serotonine (1 to 10mM) in the male gonad leads to release of sperm in sea water. By dilution into regular sea water, live oyster spermatozoa obtained by scarification show initially mostly erratic movements with poor efficiency for forward translation and initiate efficient flagellar motility after several minutes.Motility of black-lip pearl oyster spermatozoa appears gradually when spermatozoa are transferred into alkaline sea water. This motility-activating effect is reversed when pH is shifted back to acidic values; as in normal sea water, activity of sperm (% motile cells) increases gradually after alkaline pH activation and lasts for several minutes. The characteristics of these fully motile spermatozoa are described in ... |
author2 |
University of French Polynesia UMR PFOM Department, Station Expérimental d'Argenton Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Physiologie et Génomique des Poissons (LPGP) Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) UMR PFOM Department, Station Expérimentale d'Argenton Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology University of South Bohemia CENAQUA (registration number CZ.1.05/2.1.00/01.0024. of Czech Republic) - Czech Agencies Projects: IAA 608030801, ME10015, QH82119, LC06073, CZ.1.05/2.1.00/01.0024, GACR P502/11/0090, GAJU 046/2010/Z Brenda T. Erickson |
format |
Book Part |
author |
Demoy-Schneider, Marina Schmitt, Nelly Suquet, Marc Labbé, Catherine Boulais, Myrina Prokopchuk, Galina Cosson, Jacky |
author_facet |
Demoy-Schneider, Marina Schmitt, Nelly Suquet, Marc Labbé, Catherine Boulais, Myrina Prokopchuk, Galina Cosson, Jacky |
author_sort |
Demoy-Schneider, Marina |
title |
Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the Black-Lip Pearl oyster, Pinctada margaritifera |
title_short |
Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the Black-Lip Pearl oyster, Pinctada margaritifera |
title_full |
Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the Black-Lip Pearl oyster, Pinctada margaritifera |
title_fullStr |
Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the Black-Lip Pearl oyster, Pinctada margaritifera |
title_full_unstemmed |
Biological characteristics of sperm in two oyster species: the Pacific oyster, Crassostrea gigas, and the Black-Lip Pearl oyster, Pinctada margaritifera |
title_sort |
biological characteristics of sperm in two oyster species: the pacific oyster, crassostrea gigas, and the black-lip pearl oyster, pinctada margaritifera |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.archives-ouvertes.fr/hal-01205080 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Spermatozoa https://hal.archives-ouvertes.fr/hal-01205080 Spermatozoa, Nova Science Publishers, 259 p., 2014, 978-1-63117-020-1 https://www.novapublishers.com/catalog/product_info.php?products_id=47602 |
op_relation |
ISBN: 978-1-63117-020-1 hal-01205080 https://hal.archives-ouvertes.fr/hal-01205080 PRODINRA: 254710 |
_version_ |
1766394827889442816 |