Functional divergence of type 2 deiodinase paralogs in the Atlantic salmon
Thyroid hormone (TH) is an ancestral signal linked to seasonal life history transitions throughout vertebrates. TH action depends upon tissue-localized regulation of levels of active TH (triiodothyronine, T3), through spatiotemporal expression of thyroid hormone deiodinase (dio) genes. We investigat...
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Online Access: | https://hal.archives-ouvertes.fr/hal-01194956 https://doi.org/10.1016/j.cub.2015.01.074 |
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ftccsdartic:oai:HAL:hal-01194956v1 2023-05-15T15:31:23+02:00 Functional divergence of type 2 deiodinase paralogs in the Atlantic salmon Lorgen, Marlene Casadei, Elisa Król, Elżbieta Douglas, Alex Birnie, Mike J. Ebbesson, Lars O.E. Nilsen, Tom O. Jordan, William C. Jørgensen, Even H. Dardente, Hugues Hazlerigg, David G. Martin, Samuel A. M. Institute of Biological and Environmental Sciences University of Aberdeen Uni Research Environment Uni Research Ltd Institute of Zoology Zoological Society of London Department of Arctic and Marine Biology, Faculty of BioSciences, Fisheries and Economy University of Tromsø (UiT) Physiologie de la reproduction et des comportements Nouzilly (PRC) Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation Saumur -Université de Tours-Centre National de la Recherche Scientifique (CNRS) Biotechnology and Biological Sciences Research Council (BB/H008063/1) UK to D.G.H.; S.A.M.M. and the Research Council Norway (project #222215) to L.O.E.E. M.L. was funded by a Marine Alliance for Science and Technology for Scotland 2015 https://hal.archives-ouvertes.fr/hal-01194956 https://doi.org/10.1016/j.cub.2015.01.074 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cub.2015.01.074 hal-01194956 https://hal.archives-ouvertes.fr/hal-01194956 doi:10.1016/j.cub.2015.01.074 PRODINRA: 286340 WOS: 000352091100031 ISSN: 0960-9822 EISSN: 1879-0445 Current Biology - CB https://hal.archives-ouvertes.fr/hal-01194956 Current Biology - CB, Elsevier, 2015, 25 (7), pp.936-941. ⟨10.1016/j.cub.2015.01.074⟩ [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] info:eu-repo/semantics/article Journal articles 2015 ftccsdartic https://doi.org/10.1016/j.cub.2015.01.074 2021-12-05T03:06:39Z Thyroid hormone (TH) is an ancestral signal linked to seasonal life history transitions throughout vertebrates. TH action depends upon tissue-localized regulation of levels of active TH (triiodothyronine, T3), through spatiotemporal expression of thyroid hormone deiodinase (dio) genes. We investigated the dio gene family in juvenile Atlantic salmon (Salmo salar) parr, which prepare for seaward migration in the spring (smoltification) through TH-dependent changes in physiology. We identified two type 2 deiodinase paralogs, dio2a and dio2b, responsible for conversion of thyroxine (T4) to T3. During smoltification, dio2b was induced in the brain and gills in zones of cell proliferation following increasing day length. Contrastingly, dio2a expression was induced in the gills by transfer to salt water (SW), with the magnitude of the response proportional to the plasma chloride level. This response reflected a selective enrichment for osmotic response elements (OREs) in the dio2a promoter region. Transcriptomic profiling of gill tissue from fish transferred to SW plus or minus the deiodinase inhibitor, iopanoic acid, revealed SW-induced increases in cellular respiration as the principal consequence of gill dio2 activity. Divergent evolution of dio2 paralogs supports organ-specific timing of the TH-dependent events governing the phenotypic plasticity required for migration to sea. Article in Journal/Newspaper Atlantic salmon Salmo salar Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Current Biology 25 7 936 941 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] |
spellingShingle |
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Lorgen, Marlene Casadei, Elisa Król, Elżbieta Douglas, Alex Birnie, Mike J. Ebbesson, Lars O.E. Nilsen, Tom O. Jordan, William C. Jørgensen, Even H. Dardente, Hugues Hazlerigg, David G. Martin, Samuel A. M. Functional divergence of type 2 deiodinase paralogs in the Atlantic salmon |
topic_facet |
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] |
description |
Thyroid hormone (TH) is an ancestral signal linked to seasonal life history transitions throughout vertebrates. TH action depends upon tissue-localized regulation of levels of active TH (triiodothyronine, T3), through spatiotemporal expression of thyroid hormone deiodinase (dio) genes. We investigated the dio gene family in juvenile Atlantic salmon (Salmo salar) parr, which prepare for seaward migration in the spring (smoltification) through TH-dependent changes in physiology. We identified two type 2 deiodinase paralogs, dio2a and dio2b, responsible for conversion of thyroxine (T4) to T3. During smoltification, dio2b was induced in the brain and gills in zones of cell proliferation following increasing day length. Contrastingly, dio2a expression was induced in the gills by transfer to salt water (SW), with the magnitude of the response proportional to the plasma chloride level. This response reflected a selective enrichment for osmotic response elements (OREs) in the dio2a promoter region. Transcriptomic profiling of gill tissue from fish transferred to SW plus or minus the deiodinase inhibitor, iopanoic acid, revealed SW-induced increases in cellular respiration as the principal consequence of gill dio2 activity. Divergent evolution of dio2 paralogs supports organ-specific timing of the TH-dependent events governing the phenotypic plasticity required for migration to sea. |
author2 |
Institute of Biological and Environmental Sciences University of Aberdeen Uni Research Environment Uni Research Ltd Institute of Zoology Zoological Society of London Department of Arctic and Marine Biology, Faculty of BioSciences, Fisheries and Economy University of Tromsø (UiT) Physiologie de la reproduction et des comportements Nouzilly (PRC) Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation Saumur -Université de Tours-Centre National de la Recherche Scientifique (CNRS) Biotechnology and Biological Sciences Research Council (BB/H008063/1) UK to D.G.H.; S.A.M.M. and the Research Council Norway (project #222215) to L.O.E.E. M.L. was funded by a Marine Alliance for Science and Technology for Scotland |
format |
Article in Journal/Newspaper |
author |
Lorgen, Marlene Casadei, Elisa Król, Elżbieta Douglas, Alex Birnie, Mike J. Ebbesson, Lars O.E. Nilsen, Tom O. Jordan, William C. Jørgensen, Even H. Dardente, Hugues Hazlerigg, David G. Martin, Samuel A. M. |
author_facet |
Lorgen, Marlene Casadei, Elisa Król, Elżbieta Douglas, Alex Birnie, Mike J. Ebbesson, Lars O.E. Nilsen, Tom O. Jordan, William C. Jørgensen, Even H. Dardente, Hugues Hazlerigg, David G. Martin, Samuel A. M. |
author_sort |
Lorgen, Marlene |
title |
Functional divergence of type 2 deiodinase paralogs in the Atlantic salmon |
title_short |
Functional divergence of type 2 deiodinase paralogs in the Atlantic salmon |
title_full |
Functional divergence of type 2 deiodinase paralogs in the Atlantic salmon |
title_fullStr |
Functional divergence of type 2 deiodinase paralogs in the Atlantic salmon |
title_full_unstemmed |
Functional divergence of type 2 deiodinase paralogs in the Atlantic salmon |
title_sort |
functional divergence of type 2 deiodinase paralogs in the atlantic salmon |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.archives-ouvertes.fr/hal-01194956 https://doi.org/10.1016/j.cub.2015.01.074 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 0960-9822 EISSN: 1879-0445 Current Biology - CB https://hal.archives-ouvertes.fr/hal-01194956 Current Biology - CB, Elsevier, 2015, 25 (7), pp.936-941. ⟨10.1016/j.cub.2015.01.074⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cub.2015.01.074 hal-01194956 https://hal.archives-ouvertes.fr/hal-01194956 doi:10.1016/j.cub.2015.01.074 PRODINRA: 286340 WOS: 000352091100031 |
op_doi |
https://doi.org/10.1016/j.cub.2015.01.074 |
container_title |
Current Biology |
container_volume |
25 |
container_issue |
7 |
container_start_page |
936 |
op_container_end_page |
941 |
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1766361884465823744 |