Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum.
International audience Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two differe...
Published in: | Aquatic Toxicology |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2014
|
Subjects: | |
Online Access: | https://hal.univ-brest.fr/hal-00948644 https://doi.org/10.1016/j.aquatox.2013.12.012 |
id |
ftccsdartic:oai:HAL:hal-00948644v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Toxin accumulation Physiological effects Harmful algae Metals Oysters ACL [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Toxin accumulation Physiological effects Harmful algae Metals Oysters ACL [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology Haberkorn, Hansy Lambert, Christophe Le Goïc, Nelly Quéré, Claudie Bruneau, Audrey Riso, Ricardo, Auffret, Michel Soudant, Philippe Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum. |
topic_facet |
Toxin accumulation Physiological effects Harmful algae Metals Oysters ACL [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in diacylglycerol - DAG - and decrease in sterols) and phospholipids (decreases in phosphatidylcholine, phosphatidylethanolamine, cardiolipin and ceramide aminoethylphosphonate) of digestive gland suggesting that lipid metabolism disruptions and/or lipid peroxidation have occurred. Simultaneously, concentrations, percentages of dead cells and phenoloxidase activity of hemocytes increased in oysters exposed to metals while reactive oxygen species production of hemocytes decreased. Feeding on the harmful dinoflagellate A. minutum resulted in significant decreases in monoacylglycerol (MAG) and DAG and ether glycerides (EG), as well as significant increases in hemocyte concentration and phagocytic activity as compared to oysters fed T. lutea. Finally, the present study revealed that short-term, simultaneous exposure to Cd-Cu and A. minutum may induce antagonistic (i.e. hemocyte concentration and phagocytosis) or synergic (i.e. DAG content in digestive gland) effects upon cellular and tissular functions in oysters. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie des Invertébrés Plouzané (LPI) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) ANR-06-SEST-0023,MODECOPHY,Modélisation des mécanismes de contamination des coquillages par des phycotoxines(2006) |
format |
Article in Journal/Newspaper |
author |
Haberkorn, Hansy Lambert, Christophe Le Goïc, Nelly Quéré, Claudie Bruneau, Audrey Riso, Ricardo, Auffret, Michel Soudant, Philippe |
author_facet |
Haberkorn, Hansy Lambert, Christophe Le Goïc, Nelly Quéré, Claudie Bruneau, Audrey Riso, Ricardo, Auffret, Michel Soudant, Philippe |
author_sort |
Haberkorn, Hansy |
title |
Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum. |
title_short |
Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum. |
title_full |
Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum. |
title_fullStr |
Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum. |
title_full_unstemmed |
Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum. |
title_sort |
cellular and biochemical responses of the oyster crassostrea gigas to controlled exposures to metals and alexandrium minutum. |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.univ-brest.fr/hal-00948644 https://doi.org/10.1016/j.aquatox.2013.12.012 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00948644 Aquatic Toxicology, Elsevier, 2014, 147, pp.158-67. ⟨10.1016/j.aquatox.2013.12.012⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2013.12.012 info:eu-repo/semantics/altIdentifier/pmid/24418747 hal-00948644 https://hal.univ-brest.fr/hal-00948644 doi:10.1016/j.aquatox.2013.12.012 PUBMED: 24418747 |
op_doi |
https://doi.org/10.1016/j.aquatox.2013.12.012 |
container_title |
Aquatic Toxicology |
container_volume |
147 |
container_start_page |
158 |
op_container_end_page |
167 |
_version_ |
1766394094293090304 |
spelling |
ftccsdartic:oai:HAL:hal-00948644v1 2023-05-15T15:58:22+02:00 Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum. Haberkorn, Hansy Lambert, Christophe Le Goïc, Nelly Quéré, Claudie Bruneau, Audrey Riso, Ricardo, Auffret, Michel Soudant, Philippe Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie des Invertébrés Plouzané (LPI) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) ANR-06-SEST-0023,MODECOPHY,Modélisation des mécanismes de contamination des coquillages par des phycotoxines(2006) 2014-02 https://hal.univ-brest.fr/hal-00948644 https://doi.org/10.1016/j.aquatox.2013.12.012 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2013.12.012 info:eu-repo/semantics/altIdentifier/pmid/24418747 hal-00948644 https://hal.univ-brest.fr/hal-00948644 doi:10.1016/j.aquatox.2013.12.012 PUBMED: 24418747 ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00948644 Aquatic Toxicology, Elsevier, 2014, 147, pp.158-67. ⟨10.1016/j.aquatox.2013.12.012⟩ Toxin accumulation Physiological effects Harmful algae Metals Oysters ACL [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2014 ftccsdartic https://doi.org/10.1016/j.aquatox.2013.12.012 2021-12-19T03:08:22Z International audience Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in diacylglycerol - DAG - and decrease in sterols) and phospholipids (decreases in phosphatidylcholine, phosphatidylethanolamine, cardiolipin and ceramide aminoethylphosphonate) of digestive gland suggesting that lipid metabolism disruptions and/or lipid peroxidation have occurred. Simultaneously, concentrations, percentages of dead cells and phenoloxidase activity of hemocytes increased in oysters exposed to metals while reactive oxygen species production of hemocytes decreased. Feeding on the harmful dinoflagellate A. minutum resulted in significant decreases in monoacylglycerol (MAG) and DAG and ether glycerides (EG), as well as significant increases in hemocyte concentration and phagocytic activity as compared to oysters fed T. lutea. Finally, the present study revealed that short-term, simultaneous exposure to Cd-Cu and A. minutum may induce antagonistic (i.e. hemocyte concentration and phagocytosis) or synergic (i.e. DAG content in digestive gland) effects upon cellular and tissular functions in oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Aquatic Toxicology 147 158 167 |