The Arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics
International audience The Arctic seasonal snowpack can extend at times over a third of the Earth's land surface. This chemically dynamic environment interacts constantly with different environmental compartments such as the atmosphere, soil and snow meltwater, and thus, strongly influences the...
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ftccsdartic:oai:HAL:hal-00932231v1 2023-05-15T14:52:01+02:00 The Arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics Maccario, Lorrie Vogel, Timothy M. Larose, Catherine Ampère, Département Bioingénierie (BioIng) Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL) Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Big Sky, Montana, United States 2013-09-08 https://hal.archives-ouvertes.fr/hal-00932231 en eng HAL CCSD hal-00932231 https://hal.archives-ouvertes.fr/hal-00932231 PAM5 https://hal.archives-ouvertes.fr/hal-00932231 PAM5, Sep 2013, Big Sky, Montana, United States. pp.36 [SPI.NRJ]Engineering Sciences [physics]/Electric power [SDE]Environmental Sciences info:eu-repo/semantics/conferenceObject Conference papers 2013 ftccsdartic 2021-10-17T01:35:14Z International audience The Arctic seasonal snowpack can extend at times over a third of the Earth's land surface. This chemically dynamic environment interacts constantly with different environmental compartments such as the atmosphere, soil and snow meltwater, and thus, strongly influences the entire biosphere. However, the microbial community associated with this habitat and its potential role in biogeochemical cycling remains poorly understood. Previous studies based on 16S rRNA gene analysis revealed a high diversity of microorganisms within the snowpack. Here, we focused on both microbial community structure and function by applying a global approach using metagenomics and metatranscriptomics. From the 250 thousand sequence reads in all our snow metagenomes, the majority (between 58 and 88%) were unassigned to specific metabolic categories, which signals the lack of related functional data in sequence databases. However, snow metagenome analyses demonstrated major shifts in function distribution during the season indicating that the snowpack is a dynamic ecosystem. These changes seem to be related to fluctuations in environmental conditions as some chemical parameters, like mercury or methyl-mercury concentrations, were correlated with function abundance. Comparing snow metagenomes with publically available datasets from different ecosystems, we described the specific functional signature of snowpack microbial community. Some functions like oxidative stress response or lipopolysaccharides biosynthesis are more highly represented in snow metagenomes than in those from other ecosystems. These functions are probably related to how microorganisms cope with the harsh conditions (such as intense UV radiation and cold temperatures) characteristic of the Arctic snowpack. The extraction of mRNA from the Arctic snowpack for further metatranscriptomic analyses supports the hypothesis that microorganisms are metabolically active in the polar snow ecosystem. The expression pattern derived from the mRNA sequencing ... Conference Object Arctic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SPI.NRJ]Engineering Sciences [physics]/Electric power [SDE]Environmental Sciences |
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[SPI.NRJ]Engineering Sciences [physics]/Electric power [SDE]Environmental Sciences Maccario, Lorrie Vogel, Timothy M. Larose, Catherine The Arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics |
topic_facet |
[SPI.NRJ]Engineering Sciences [physics]/Electric power [SDE]Environmental Sciences |
description |
International audience The Arctic seasonal snowpack can extend at times over a third of the Earth's land surface. This chemically dynamic environment interacts constantly with different environmental compartments such as the atmosphere, soil and snow meltwater, and thus, strongly influences the entire biosphere. However, the microbial community associated with this habitat and its potential role in biogeochemical cycling remains poorly understood. Previous studies based on 16S rRNA gene analysis revealed a high diversity of microorganisms within the snowpack. Here, we focused on both microbial community structure and function by applying a global approach using metagenomics and metatranscriptomics. From the 250 thousand sequence reads in all our snow metagenomes, the majority (between 58 and 88%) were unassigned to specific metabolic categories, which signals the lack of related functional data in sequence databases. However, snow metagenome analyses demonstrated major shifts in function distribution during the season indicating that the snowpack is a dynamic ecosystem. These changes seem to be related to fluctuations in environmental conditions as some chemical parameters, like mercury or methyl-mercury concentrations, were correlated with function abundance. Comparing snow metagenomes with publically available datasets from different ecosystems, we described the specific functional signature of snowpack microbial community. Some functions like oxidative stress response or lipopolysaccharides biosynthesis are more highly represented in snow metagenomes than in those from other ecosystems. These functions are probably related to how microorganisms cope with the harsh conditions (such as intense UV radiation and cold temperatures) characteristic of the Arctic snowpack. The extraction of mRNA from the Arctic snowpack for further metatranscriptomic analyses supports the hypothesis that microorganisms are metabolically active in the polar snow ecosystem. The expression pattern derived from the mRNA sequencing ... |
author2 |
Ampère, Département Bioingénierie (BioIng) Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL) Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
format |
Conference Object |
author |
Maccario, Lorrie Vogel, Timothy M. Larose, Catherine |
author_facet |
Maccario, Lorrie Vogel, Timothy M. Larose, Catherine |
author_sort |
Maccario, Lorrie |
title |
The Arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics |
title_short |
The Arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics |
title_full |
The Arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics |
title_fullStr |
The Arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics |
title_full_unstemmed |
The Arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics |
title_sort |
arctic snowpack microbial community highlighted by metagenomics and metatranscriptomics |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.archives-ouvertes.fr/hal-00932231 |
op_coverage |
Big Sky, Montana, United States |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PAM5 https://hal.archives-ouvertes.fr/hal-00932231 PAM5, Sep 2013, Big Sky, Montana, United States. pp.36 |
op_relation |
hal-00932231 https://hal.archives-ouvertes.fr/hal-00932231 |
_version_ |
1766323150975401984 |