A multibiomarker approach in juvenile turbot, Scophthalmus maximus, exposed to contaminated sediments
International audience Juvenile turbot were exposed in laboratory conditions to a mixture of chemical contaminants associated with harbour and estuarine sediments for seven and 21 days. Several molecular biomarkers of exposure were then measured in fish liver: two biotransformation parameters [ethox...
Published in: | Ecotoxicology and Environmental Safety |
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Online Access: | https://hal.archives-ouvertes.fr/hal-00815307 https://hal.archives-ouvertes.fr/hal-00815307/document https://hal.archives-ouvertes.fr/hal-00815307/file/2012-021_post-print.pdf https://doi.org/10.1016/j.ecoenv.2012.02.010 |
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ftccsdartic:oai:HAL:hal-00815307v1 2023-05-15T18:15:52+02:00 A multibiomarker approach in juvenile turbot, Scophthalmus maximus, exposed to contaminated sediments Kerambrun, Elodie Henry, Françoise Marechal, A. Sanchez, W. Filipuci, I. Minier, C. Amara, Rachid Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG) Centre National de la Recherche Scientifique (CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut national des sciences de l'Univers (INSU - CNRS) Laboratoire d'Ecotoxicologie - Milieux Aquatiques (LEMA) Université Le Havre Normandie (ULH) Normandie Université (NU)-Normandie Université (NU) Institut National de l'Environnement Industriel et des Risques (INERIS) 2012-06 https://hal.archives-ouvertes.fr/hal-00815307 https://hal.archives-ouvertes.fr/hal-00815307/document https://hal.archives-ouvertes.fr/hal-00815307/file/2012-021_post-print.pdf https://doi.org/10.1016/j.ecoenv.2012.02.010 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2012.02.010 hal-00815307 https://hal.archives-ouvertes.fr/hal-00815307 https://hal.archives-ouvertes.fr/hal-00815307/document https://hal.archives-ouvertes.fr/hal-00815307/file/2012-021_post-print.pdf doi:10.1016/j.ecoenv.2012.02.010 info:eu-repo/semantics/OpenAccess ISSN: 0147-6513 EISSN: 1090-2414 Ecotoxicology and Environmental Safety https://hal.archives-ouvertes.fr/hal-00815307 Ecotoxicology and Environmental Safety, Elsevier, 2012, 80, pp.45-53. ⟨10.1016/j.ecoenv.2012.02.010⟩ [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2012 ftccsdartic https://doi.org/10.1016/j.ecoenv.2012.02.010 2021-11-28T01:24:23Z International audience Juvenile turbot were exposed in laboratory conditions to a mixture of chemical contaminants associated with harbour and estuarine sediments for seven and 21 days. Several molecular biomarkers of exposure were then measured in fish liver: two biotransformation parameters [ethoxyresorufin-O-deethylase (EROD) and phase II glutathione S-transferase (GST) activities] and an antioxidant enzyme activity [catalase (CAT)]. Modifications at the histological level were analysed by the measurement of the number and size of melanomacrophage centres (MMCs) and disturbances to the immune function by the measurement of cytokine transforming growth factor-beta (TGF-β) and development of the thymus. The responses of these molecular and immunological biomarkers were correlated with metal and PAH concentrations measured in sediments and with the physiological performance of turbot analysed in a previous study on the same fish (growth rates, condition factor K, RNA:DNA ratio and lipid index). While no difference was found in thymus analysis, some molecular and immunological responses were observed in fish exposed to contaminated sediments. Weak relationships between molecular biomarkers' responses and PAH concentrations were recorded, while their responses were significantly correlated with some metals. MMC and aggregates were weakly related to chemical contaminants whereas some significant correlations were found between TGF-b1 responses and some metal concentrations. However, molecular and immunological biomarkers were weakly related to fish physiological damages since low responses were observed in the condition which led to the lowest growth and condition indices. These data suggest the complexity of cause-effect relationships between exposure to pollutants, metabolisms and health damages. Precautions should be considered in the use of molecular and immunological biomarkers alone in biomonitoring programs. Their complementary use with physiological biomarkers, such as fish growth and condition indices, ... Article in Journal/Newspaper Scophthalmus maximus Turbot Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Ecotoxicology and Environmental Safety 80 45 53 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Kerambrun, Elodie Henry, Françoise Marechal, A. Sanchez, W. Filipuci, I. Minier, C. Amara, Rachid A multibiomarker approach in juvenile turbot, Scophthalmus maximus, exposed to contaminated sediments |
topic_facet |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience Juvenile turbot were exposed in laboratory conditions to a mixture of chemical contaminants associated with harbour and estuarine sediments for seven and 21 days. Several molecular biomarkers of exposure were then measured in fish liver: two biotransformation parameters [ethoxyresorufin-O-deethylase (EROD) and phase II glutathione S-transferase (GST) activities] and an antioxidant enzyme activity [catalase (CAT)]. Modifications at the histological level were analysed by the measurement of the number and size of melanomacrophage centres (MMCs) and disturbances to the immune function by the measurement of cytokine transforming growth factor-beta (TGF-β) and development of the thymus. The responses of these molecular and immunological biomarkers were correlated with metal and PAH concentrations measured in sediments and with the physiological performance of turbot analysed in a previous study on the same fish (growth rates, condition factor K, RNA:DNA ratio and lipid index). While no difference was found in thymus analysis, some molecular and immunological responses were observed in fish exposed to contaminated sediments. Weak relationships between molecular biomarkers' responses and PAH concentrations were recorded, while their responses were significantly correlated with some metals. MMC and aggregates were weakly related to chemical contaminants whereas some significant correlations were found between TGF-b1 responses and some metal concentrations. However, molecular and immunological biomarkers were weakly related to fish physiological damages since low responses were observed in the condition which led to the lowest growth and condition indices. These data suggest the complexity of cause-effect relationships between exposure to pollutants, metabolisms and health damages. Precautions should be considered in the use of molecular and immunological biomarkers alone in biomonitoring programs. Their complementary use with physiological biomarkers, such as fish growth and condition indices, ... |
author2 |
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG) Centre National de la Recherche Scientifique (CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut national des sciences de l'Univers (INSU - CNRS) Laboratoire d'Ecotoxicologie - Milieux Aquatiques (LEMA) Université Le Havre Normandie (ULH) Normandie Université (NU)-Normandie Université (NU) Institut National de l'Environnement Industriel et des Risques (INERIS) |
format |
Article in Journal/Newspaper |
author |
Kerambrun, Elodie Henry, Françoise Marechal, A. Sanchez, W. Filipuci, I. Minier, C. Amara, Rachid |
author_facet |
Kerambrun, Elodie Henry, Françoise Marechal, A. Sanchez, W. Filipuci, I. Minier, C. Amara, Rachid |
author_sort |
Kerambrun, Elodie |
title |
A multibiomarker approach in juvenile turbot, Scophthalmus maximus, exposed to contaminated sediments |
title_short |
A multibiomarker approach in juvenile turbot, Scophthalmus maximus, exposed to contaminated sediments |
title_full |
A multibiomarker approach in juvenile turbot, Scophthalmus maximus, exposed to contaminated sediments |
title_fullStr |
A multibiomarker approach in juvenile turbot, Scophthalmus maximus, exposed to contaminated sediments |
title_full_unstemmed |
A multibiomarker approach in juvenile turbot, Scophthalmus maximus, exposed to contaminated sediments |
title_sort |
multibiomarker approach in juvenile turbot, scophthalmus maximus, exposed to contaminated sediments |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.archives-ouvertes.fr/hal-00815307 https://hal.archives-ouvertes.fr/hal-00815307/document https://hal.archives-ouvertes.fr/hal-00815307/file/2012-021_post-print.pdf https://doi.org/10.1016/j.ecoenv.2012.02.010 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
ISSN: 0147-6513 EISSN: 1090-2414 Ecotoxicology and Environmental Safety https://hal.archives-ouvertes.fr/hal-00815307 Ecotoxicology and Environmental Safety, Elsevier, 2012, 80, pp.45-53. ⟨10.1016/j.ecoenv.2012.02.010⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2012.02.010 hal-00815307 https://hal.archives-ouvertes.fr/hal-00815307 https://hal.archives-ouvertes.fr/hal-00815307/document https://hal.archives-ouvertes.fr/hal-00815307/file/2012-021_post-print.pdf doi:10.1016/j.ecoenv.2012.02.010 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.ecoenv.2012.02.010 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
80 |
container_start_page |
45 |
op_container_end_page |
53 |
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1766189113502859264 |