Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas.
International audience We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-la...
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Online Access: | https://hal.univ-brest.fr/hal-00788236 https://doi.org/10.1016/j.jprot.2012.07.040 |
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ftccsdartic:oai:HAL:hal-00788236v1 2023-05-15T15:57:43+02:00 Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas. Corporeau, Charlotte Vanderplancke, Gwenaëlle Boulais, Myrina Suquet, Marc Quéré, Claudie Boudry, Pierre Huvet, Arnaud Madec, Stéphanie Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) 2012-10-22 https://hal.univ-brest.fr/hal-00788236 https://doi.org/10.1016/j.jprot.2012.07.040 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2012.07.040 info:eu-repo/semantics/altIdentifier/pmid/22878033 hal-00788236 https://hal.univ-brest.fr/hal-00788236 doi:10.1016/j.jprot.2012.07.040 PUBMED: 22878033 ISSN: 1874-3919 EISSN: 1876-7737 Journal of Proteomics https://hal.univ-brest.fr/hal-00788236 Journal of Proteomics, Elsevier, 2012, 75 (18), pp.5554-63. ⟨10.1016/j.jprot.2012.07.040⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftccsdartic https://doi.org/10.1016/j.jprot.2012.07.040 2021-12-19T03:20:36Z International audience We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin - breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and cell-cycle control proteins. This is the first proteomic study dedicated to oocytes in C. gigas. Our results improve current knowledge about protein factors associated with oocyte quality in this species, and our understanding of the proteomic processes involved in their developmental competence. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Journal of Proteomics 75 18 5554 5563 |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Corporeau, Charlotte Vanderplancke, Gwenaëlle Boulais, Myrina Suquet, Marc Quéré, Claudie Boudry, Pierre Huvet, Arnaud Madec, Stéphanie Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas. |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin - breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and cell-cycle control proteins. This is the first proteomic study dedicated to oocytes in C. gigas. Our results improve current knowledge about protein factors associated with oocyte quality in this species, and our understanding of the proteomic processes involved in their developmental competence. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) |
format |
Article in Journal/Newspaper |
author |
Corporeau, Charlotte Vanderplancke, Gwenaëlle Boulais, Myrina Suquet, Marc Quéré, Claudie Boudry, Pierre Huvet, Arnaud Madec, Stéphanie |
author_facet |
Corporeau, Charlotte Vanderplancke, Gwenaëlle Boulais, Myrina Suquet, Marc Quéré, Claudie Boudry, Pierre Huvet, Arnaud Madec, Stéphanie |
author_sort |
Corporeau, Charlotte |
title |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas. |
title_short |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas. |
title_full |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas. |
title_fullStr |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas. |
title_full_unstemmed |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas. |
title_sort |
proteomic identification of quality factors for oocytes in the pacific oyster crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00788236 https://doi.org/10.1016/j.jprot.2012.07.040 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1874-3919 EISSN: 1876-7737 Journal of Proteomics https://hal.univ-brest.fr/hal-00788236 Journal of Proteomics, Elsevier, 2012, 75 (18), pp.5554-63. ⟨10.1016/j.jprot.2012.07.040⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2012.07.040 info:eu-repo/semantics/altIdentifier/pmid/22878033 hal-00788236 https://hal.univ-brest.fr/hal-00788236 doi:10.1016/j.jprot.2012.07.040 PUBMED: 22878033 |
op_doi |
https://doi.org/10.1016/j.jprot.2012.07.040 |
container_title |
Journal of Proteomics |
container_volume |
75 |
container_issue |
18 |
container_start_page |
5554 |
op_container_end_page |
5563 |
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1766393404890021888 |