Effect of increased pCO(2) on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms.
International audience Ocean acidification may stimulate primary production through increased availability of inorganic carbon in the photic zone, which may in turn change the biogenic flux of dissolved organic carbon (DOC) and the growth potential of heterotrophic bacteria. To investigate the effec...
Published in: | FEMS Microbiology Ecology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hal.archives-ouvertes.fr/hal-00757197 https://doi.org/10.1111/j.1574-6941.2012.01443.x |
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ftccsdartic:oai:HAL:hal-00757197v1 2023-05-15T16:03:50+02:00 Effect of increased pCO(2) on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms. Ray, Jessica L Töpper, Birte An, Shu Silyakova, Anna Spindelböck, Joachim Thyrhaug, Runar Dubow, Michael S Thingstad, T Frede Sandaa, Ruth-Anne Institut de génétique et microbiologie Orsay (IGM) Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) 2012-12 https://hal.archives-ouvertes.fr/hal-00757197 https://doi.org/10.1111/j.1574-6941.2012.01443.x en eng HAL CCSD Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6941.2012.01443.x info:eu-repo/semantics/altIdentifier/pmid/22775552 hal-00757197 https://hal.archives-ouvertes.fr/hal-00757197 doi:10.1111/j.1574-6941.2012.01443.x PUBMED: 22775552 ISSN: 0168-6496 EISSN: 1574-6941 FEMS Microbiology Ecology https://hal.archives-ouvertes.fr/hal-00757197 FEMS Microbiology Ecology, Wiley-Blackwell, 2012, 82 (3), pp.713-23. ⟨10.1111/j.1574-6941.2012.01443.x⟩ [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology info:eu-repo/semantics/article Journal articles 2012 ftccsdartic https://doi.org/10.1111/j.1574-6941.2012.01443.x 2021-07-04T02:00:24Z International audience Ocean acidification may stimulate primary production through increased availability of inorganic carbon in the photic zone, which may in turn change the biogenic flux of dissolved organic carbon (DOC) and the growth potential of heterotrophic bacteria. To investigate the effects of ocean acidification on marine bacterial assemblages, a two-by-three factorial mescosom experiment was conducted using surface sea water from the East Greenland Current in Fram Strait. Pyrosequencing of the V1-V2 region of bacterial 16S ribosomal RNA genes was used to investigate differences in the endpoint (Day 9) composition of bacterial assemblages in mineral nutrient-replete mesocosms amended with glucose (0 μm, 5.3 μm and 15.9 μm) under ambient (250 μatm) or acidified (400 μatm) partial pressures of CO(2) (pCO(2) ). All mesocosms showed low richness and diversity by Chao1 estimator and Shannon index, respectively, with general dominance by Gammaproteobacteria and Flavobacteria. Nonmetric multidimensional scaling analysis and two-way analysis of variance of the Jaccard dissimilarity matrix (97% similarity cut-off) demonstrated that the significant community shift between 0 μm and 15.9 μm glucose addition at 250 μatm pCO(2) was eliminated at 400 μatm pCO(2) . These results suggest that the response potential of marine bacteria to DOC input may be altered under acidified conditions. Article in Journal/Newspaper East Greenland east greenland current Fram Strait Greenland Ocean acidification Shannon Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Greenland FEMS Microbiology Ecology 82 3 713 723 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology |
spellingShingle |
[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Ray, Jessica L Töpper, Birte An, Shu Silyakova, Anna Spindelböck, Joachim Thyrhaug, Runar Dubow, Michael S Thingstad, T Frede Sandaa, Ruth-Anne Effect of increased pCO(2) on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms. |
topic_facet |
[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology |
description |
International audience Ocean acidification may stimulate primary production through increased availability of inorganic carbon in the photic zone, which may in turn change the biogenic flux of dissolved organic carbon (DOC) and the growth potential of heterotrophic bacteria. To investigate the effects of ocean acidification on marine bacterial assemblages, a two-by-three factorial mescosom experiment was conducted using surface sea water from the East Greenland Current in Fram Strait. Pyrosequencing of the V1-V2 region of bacterial 16S ribosomal RNA genes was used to investigate differences in the endpoint (Day 9) composition of bacterial assemblages in mineral nutrient-replete mesocosms amended with glucose (0 μm, 5.3 μm and 15.9 μm) under ambient (250 μatm) or acidified (400 μatm) partial pressures of CO(2) (pCO(2) ). All mesocosms showed low richness and diversity by Chao1 estimator and Shannon index, respectively, with general dominance by Gammaproteobacteria and Flavobacteria. Nonmetric multidimensional scaling analysis and two-way analysis of variance of the Jaccard dissimilarity matrix (97% similarity cut-off) demonstrated that the significant community shift between 0 μm and 15.9 μm glucose addition at 250 μatm pCO(2) was eliminated at 400 μatm pCO(2) . These results suggest that the response potential of marine bacteria to DOC input may be altered under acidified conditions. |
author2 |
Institut de génétique et microbiologie Orsay (IGM) Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Ray, Jessica L Töpper, Birte An, Shu Silyakova, Anna Spindelböck, Joachim Thyrhaug, Runar Dubow, Michael S Thingstad, T Frede Sandaa, Ruth-Anne |
author_facet |
Ray, Jessica L Töpper, Birte An, Shu Silyakova, Anna Spindelböck, Joachim Thyrhaug, Runar Dubow, Michael S Thingstad, T Frede Sandaa, Ruth-Anne |
author_sort |
Ray, Jessica L |
title |
Effect of increased pCO(2) on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms. |
title_short |
Effect of increased pCO(2) on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms. |
title_full |
Effect of increased pCO(2) on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms. |
title_fullStr |
Effect of increased pCO(2) on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms. |
title_full_unstemmed |
Effect of increased pCO(2) on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms. |
title_sort |
effect of increased pco(2) on bacterial assemblage shifts in response to glucose addition in fram strait seawater mesocosms. |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.archives-ouvertes.fr/hal-00757197 https://doi.org/10.1111/j.1574-6941.2012.01443.x |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
East Greenland east greenland current Fram Strait Greenland Ocean acidification Shannon |
genre_facet |
East Greenland east greenland current Fram Strait Greenland Ocean acidification Shannon |
op_source |
ISSN: 0168-6496 EISSN: 1574-6941 FEMS Microbiology Ecology https://hal.archives-ouvertes.fr/hal-00757197 FEMS Microbiology Ecology, Wiley-Blackwell, 2012, 82 (3), pp.713-23. ⟨10.1111/j.1574-6941.2012.01443.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6941.2012.01443.x info:eu-repo/semantics/altIdentifier/pmid/22775552 hal-00757197 https://hal.archives-ouvertes.fr/hal-00757197 doi:10.1111/j.1574-6941.2012.01443.x PUBMED: 22775552 |
op_doi |
https://doi.org/10.1111/j.1574-6941.2012.01443.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
82 |
container_issue |
3 |
container_start_page |
713 |
op_container_end_page |
723 |
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1766399520424329216 |