Responses of conventional and molecular biomarkers in turbot Scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene.
International audience Several accidental spills in European coastal areas have resulted in the release of different toxic compounds into the marine environment, such as heavy fuel oil type no. 6 in the "Erika" and "Prestige" oil spills and the highly toxic styrene after the loss...
Published in: | Aquatic Toxicology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hal.univ-brest.fr/hal-00753917 https://doi.org/10.1016/j.aquatox.2012.02.004 |
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ftccsdartic:oai:HAL:hal-00753917v1 2023-05-15T18:15:48+02:00 Responses of conventional and molecular biomarkers in turbot Scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene. Ruiz, Pamela Ortiz-Zarragoitia, Maren Orbea, Amaia Theron, Michael Le Floch, Stéphane Cajaraville, Miren P Optimisation des régulations physiologiques (ORPHY (EA 4324)) Institut Brestois Santé Agro Matière (IBSAM) Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest) Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre) Cedre 2012-07-15 https://hal.univ-brest.fr/hal-00753917 https://doi.org/10.1016/j.aquatox.2012.02.004 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2012.02.004 info:eu-repo/semantics/altIdentifier/pmid/22483509 hal-00753917 https://hal.univ-brest.fr/hal-00753917 doi:10.1016/j.aquatox.2012.02.004 PUBMED: 22483509 ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00753917 Aquatic Toxicology, Elsevier, 2012, 116-117, pp.116-28. ⟨10.1016/j.aquatox.2012.02.004⟩ MESH: Acyl-CoA Oxidase MESH: Animals MESH: Seawater MESH: Styrene MESH: Vitellogenins MESH: Water Pollutants Chemical MESH: Biological Markers MESH: Flatfishes MESH: Fuel Oils MESH: Gene Expression Regulation MESH: Gonads MESH: Liver MESH: Lysosomes MESH: Receptors Aryl Hydrocarbon [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] info:eu-repo/semantics/article Journal articles 2012 ftccsdartic https://doi.org/10.1016/j.aquatox.2012.02.004 2021-10-17T01:44:46Z International audience Several accidental spills in European coastal areas have resulted in the release of different toxic compounds into the marine environment, such as heavy fuel oil type no. 6 in the "Erika" and "Prestige" oil spills and the highly toxic styrene after the loss of the "Ievoli Sun". There is a clear need to develop tools that might allow assessing the biological impact of these accidental spills on aquatic organisms. The aim of the present study was to determine the short-term effects and recovery after exposure of juvenile fish (Scophthalmus maximus) to heavy fuel oil no. 6 and styrene by using a battery of molecular, cell and tissue level biomarkers. Turbots were exposed to styrene for 7 days and to the diluted soluble fraction of the oil (10%) for 14 days, and then allowed to recover in clean seawater for the same time periods. cyp1a1 transcript was overexpressed in turbots after 3 and 14 days of exposure to heavy fuel oil, whereas ahr transcription was not modulated after heavy fuel oil and styrene exposure. pparα transcription level was significantly up-regulated after 3 days of treatment with styrene. Liver activity of peroxisomal acyl-CoA oxidase (AOX) was significantly induced after 14 days of oil exposure, but it was not affected by styrene. Hepatocyte lysosomal membrane stability (LMS) was significantly reduced after exposure to both treatments, indicating that the tested compounds significantly impaired fish health. Both AOX and LMS values returned to control levels after the recovery period. No differences in gamete development were observed between fuel- or styrene- exposed fish and control fish, and vitellogenin plasma levels were low, suggesting no xenoestrogenic effects of fuel oil or styrene. While styrene did not cause any increase in the prevalence of liver histopathological alterations, prevalence of extensive cell vacuolization increased after exposure to heavy fuel oil for 14 days. In conclusion, the suite of selected biomarkers proved to be useful to determine the early ... Article in Journal/Newspaper Scophthalmus maximus Turbot Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Aquatic Toxicology 116-117 116 128 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
MESH: Acyl-CoA Oxidase MESH: Animals MESH: Seawater MESH: Styrene MESH: Vitellogenins MESH: Water Pollutants Chemical MESH: Biological Markers MESH: Flatfishes MESH: Fuel Oils MESH: Gene Expression Regulation MESH: Gonads MESH: Liver MESH: Lysosomes MESH: Receptors Aryl Hydrocarbon [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
spellingShingle |
MESH: Acyl-CoA Oxidase MESH: Animals MESH: Seawater MESH: Styrene MESH: Vitellogenins MESH: Water Pollutants Chemical MESH: Biological Markers MESH: Flatfishes MESH: Fuel Oils MESH: Gene Expression Regulation MESH: Gonads MESH: Liver MESH: Lysosomes MESH: Receptors Aryl Hydrocarbon [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Ruiz, Pamela Ortiz-Zarragoitia, Maren Orbea, Amaia Theron, Michael Le Floch, Stéphane Cajaraville, Miren P Responses of conventional and molecular biomarkers in turbot Scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene. |
topic_facet |
MESH: Acyl-CoA Oxidase MESH: Animals MESH: Seawater MESH: Styrene MESH: Vitellogenins MESH: Water Pollutants Chemical MESH: Biological Markers MESH: Flatfishes MESH: Fuel Oils MESH: Gene Expression Regulation MESH: Gonads MESH: Liver MESH: Lysosomes MESH: Receptors Aryl Hydrocarbon [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
description |
International audience Several accidental spills in European coastal areas have resulted in the release of different toxic compounds into the marine environment, such as heavy fuel oil type no. 6 in the "Erika" and "Prestige" oil spills and the highly toxic styrene after the loss of the "Ievoli Sun". There is a clear need to develop tools that might allow assessing the biological impact of these accidental spills on aquatic organisms. The aim of the present study was to determine the short-term effects and recovery after exposure of juvenile fish (Scophthalmus maximus) to heavy fuel oil no. 6 and styrene by using a battery of molecular, cell and tissue level biomarkers. Turbots were exposed to styrene for 7 days and to the diluted soluble fraction of the oil (10%) for 14 days, and then allowed to recover in clean seawater for the same time periods. cyp1a1 transcript was overexpressed in turbots after 3 and 14 days of exposure to heavy fuel oil, whereas ahr transcription was not modulated after heavy fuel oil and styrene exposure. pparα transcription level was significantly up-regulated after 3 days of treatment with styrene. Liver activity of peroxisomal acyl-CoA oxidase (AOX) was significantly induced after 14 days of oil exposure, but it was not affected by styrene. Hepatocyte lysosomal membrane stability (LMS) was significantly reduced after exposure to both treatments, indicating that the tested compounds significantly impaired fish health. Both AOX and LMS values returned to control levels after the recovery period. No differences in gamete development were observed between fuel- or styrene- exposed fish and control fish, and vitellogenin plasma levels were low, suggesting no xenoestrogenic effects of fuel oil or styrene. While styrene did not cause any increase in the prevalence of liver histopathological alterations, prevalence of extensive cell vacuolization increased after exposure to heavy fuel oil for 14 days. In conclusion, the suite of selected biomarkers proved to be useful to determine the early ... |
author2 |
Optimisation des régulations physiologiques (ORPHY (EA 4324)) Institut Brestois Santé Agro Matière (IBSAM) Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest) Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre) Cedre |
format |
Article in Journal/Newspaper |
author |
Ruiz, Pamela Ortiz-Zarragoitia, Maren Orbea, Amaia Theron, Michael Le Floch, Stéphane Cajaraville, Miren P |
author_facet |
Ruiz, Pamela Ortiz-Zarragoitia, Maren Orbea, Amaia Theron, Michael Le Floch, Stéphane Cajaraville, Miren P |
author_sort |
Ruiz, Pamela |
title |
Responses of conventional and molecular biomarkers in turbot Scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene. |
title_short |
Responses of conventional and molecular biomarkers in turbot Scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene. |
title_full |
Responses of conventional and molecular biomarkers in turbot Scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene. |
title_fullStr |
Responses of conventional and molecular biomarkers in turbot Scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene. |
title_full_unstemmed |
Responses of conventional and molecular biomarkers in turbot Scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene. |
title_sort |
responses of conventional and molecular biomarkers in turbot scophthalmus maximus exposed to heavy fuel oil no. 6 and styrene. |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00753917 https://doi.org/10.1016/j.aquatox.2012.02.004 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00753917 Aquatic Toxicology, Elsevier, 2012, 116-117, pp.116-28. ⟨10.1016/j.aquatox.2012.02.004⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2012.02.004 info:eu-repo/semantics/altIdentifier/pmid/22483509 hal-00753917 https://hal.univ-brest.fr/hal-00753917 doi:10.1016/j.aquatox.2012.02.004 PUBMED: 22483509 |
op_doi |
https://doi.org/10.1016/j.aquatox.2012.02.004 |
container_title |
Aquatic Toxicology |
container_volume |
116-117 |
container_start_page |
116 |
op_container_end_page |
128 |
_version_ |
1766189022447665152 |