In vitro effect of hydrostatic pressure exposure on hydroxyl radical production in fish red muscle.

• Published in: Redox Report
• Main Authors: Amérand, Aline, Vettier, Aurélie, Sébert, Philippe, Cann-Moisan, Christine
• Other Authors: Optimisation des régulations physiologiques (ORPHY (EA 4324)), Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Unité Haute Pression et Métabolisme, UPCI-EA3879, Université de Brest (UBO)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2005
• Subjects: MESH: Acclimatization; MESH: Anguilla; MESH: Animals; MESH: Hydrostatic Pressure; MESH: Hydroxyl Radical; MESH: Muscle Fibers; Fast-Twitch; MESH: Oxygen Consumption; MESH: Reactive Oxygen Species; MESH: Trout; [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]; Anguilla anguilla
• Online Access: https://hal.univ-brest.fr/hal-00750458; https://doi.org/10.1179/135100005X21589

International audience The effects of hydrostatic pressure on reactive oxygen species (ROS) production have been studied in vitro on fish red muscle fibres. In the eel, Anguilla anguilla, previous studies have shown that hydrostatic pressure acclimatization improves oxidative phosphorylation efficiency together with a supposed concomitant decrease in electron leak and ROS production. In order to test the hypothesis of an electron leak decrease under pressure, hydroxyl radical (HO*) production and oxygen consumption were measured on fish red muscle fibres directly exposed to hydrostatic pressure. Experiments were performed under two conditions--atmospheric pressure and hydrostatic pressure (16.1 MPa)--on eel and trout (which exhibit low- and high-pressure sensitivity, respectively). This work has permitted, first, the validation of an indirect HO* measurement (in vitro) on fish red muscle and the documentation of reference values for fish. Second, at atmospheric pressure, results show higher oxygen consumption for trout (+40%) than for eel which is accompanied by higher HO* production (+90%); in addition, both species present a positive relationship between HO* production and oxygen consumption. Hydrostatic pressure exposure reverses this relationship for eel but not for trout. These preliminary results only partially verify the proposed hypothesis and further experiments are needed.