Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes.
International audience Regulatory volume decrease (RVD) following hyposmotic stimulation was studied in isolated turbot, Scophthalmus maximus, hepatocytes. Exposed to a reduced osmolality (from 320 to 240 mosm kg(-1)), cells first swelled and then exhibited a RVD. Volume regulation was significantly...
Published in: | Journal of Comparative Physiology B |
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Online Access: | https://hal.univ-brest.fr/hal-00750437 https://doi.org/10.1007/s00360-005-0061-8 |
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ftccsdartic:oai:HAL:hal-00750437v1 2023-05-15T18:15:44+02:00 Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes. Ollivier, Hélène Pichavant, Karine Puill-Stephan, Eneour Roy, Stella Calvès, Patrick Nonnotte, Liliane Nonnotte, Guy Optimisation des régulations physiologiques (ORPHY (EA 4324)) Institut Brestois Santé Agro Matière (IBSAM) Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest) 2006-06 https://hal.univ-brest.fr/hal-00750437 https://doi.org/10.1007/s00360-005-0061-8 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00360-005-0061-8 info:eu-repo/semantics/altIdentifier/pmid/16395590 hal-00750437 https://hal.univ-brest.fr/hal-00750437 doi:10.1007/s00360-005-0061-8 PUBMED: 16395590 ISSN: 0174-1578 EISSN: 1432-136X Journal of Comparative Physiology B https://hal.univ-brest.fr/hal-00750437 Journal of Comparative Physiology B, Springer Verlag, 2006, 176 (5), pp.393-403. ⟨10.1007/s00360-005-0061-8⟩ MESH: Animals MESH: Arachidonic Acid MESH: Osmotic Pressure MESH: Potassium Channels MESH: Protein Kinases MESH: Water-Electrolyte Balance MESH: Calcium MESH: Cell Size MESH: Chloride Channels MESH: Eicosanoids MESH: Flatfishes MESH: Hepatocytes [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] info:eu-repo/semantics/article Journal articles 2006 ftccsdartic https://doi.org/10.1007/s00360-005-0061-8 2021-10-17T01:44:59Z International audience Regulatory volume decrease (RVD) following hyposmotic stimulation was studied in isolated turbot, Scophthalmus maximus, hepatocytes. Exposed to a reduced osmolality (from 320 to 240 mosm kg(-1)), cells first swelled and then exhibited a RVD. Volume regulation was significantly inhibited in presence of NPPB, 9-AC, acetazolamide, DIDS and barium. Taken together, these results could suggest that RVD operated via separate K+ and Cl- channels and probably Cl-/HCO3(-) exchanger in turbot hepatocytes. The K+/Cl- cotransporter could also be involved as furosemide and DIOA strongly inhibited the process whereas NEM, a K+/Cl- cotransporter activator, added under isosmotic conditions, led to cell shrinkage. RVD in turbot hepatocytes appeared also to depend on proteins p38 MAP kinase and tyrosine kinase but not on proteins ERK 1/2. Arachidonic acid and leukotrienes could also be involved since inhibition of synthesis of both these compounds by quinacrine and NDGA, respectively, inhibited the volume regulation. Likewise, Ca2+ has been proved to be an essential messenger as RVD was prevented in absence of Ca2+. Finally, this work provides bases for novel studies on cell volume regulation in marine teleosteans. Article in Journal/Newspaper Scophthalmus maximus Turbot Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Journal of Comparative Physiology B 176 5 393 403 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
MESH: Animals MESH: Arachidonic Acid MESH: Osmotic Pressure MESH: Potassium Channels MESH: Protein Kinases MESH: Water-Electrolyte Balance MESH: Calcium MESH: Cell Size MESH: Chloride Channels MESH: Eicosanoids MESH: Flatfishes MESH: Hepatocytes [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
spellingShingle |
MESH: Animals MESH: Arachidonic Acid MESH: Osmotic Pressure MESH: Potassium Channels MESH: Protein Kinases MESH: Water-Electrolyte Balance MESH: Calcium MESH: Cell Size MESH: Chloride Channels MESH: Eicosanoids MESH: Flatfishes MESH: Hepatocytes [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Ollivier, Hélène Pichavant, Karine Puill-Stephan, Eneour Roy, Stella Calvès, Patrick Nonnotte, Liliane Nonnotte, Guy Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes. |
topic_facet |
MESH: Animals MESH: Arachidonic Acid MESH: Osmotic Pressure MESH: Potassium Channels MESH: Protein Kinases MESH: Water-Electrolyte Balance MESH: Calcium MESH: Cell Size MESH: Chloride Channels MESH: Eicosanoids MESH: Flatfishes MESH: Hepatocytes [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
description |
International audience Regulatory volume decrease (RVD) following hyposmotic stimulation was studied in isolated turbot, Scophthalmus maximus, hepatocytes. Exposed to a reduced osmolality (from 320 to 240 mosm kg(-1)), cells first swelled and then exhibited a RVD. Volume regulation was significantly inhibited in presence of NPPB, 9-AC, acetazolamide, DIDS and barium. Taken together, these results could suggest that RVD operated via separate K+ and Cl- channels and probably Cl-/HCO3(-) exchanger in turbot hepatocytes. The K+/Cl- cotransporter could also be involved as furosemide and DIOA strongly inhibited the process whereas NEM, a K+/Cl- cotransporter activator, added under isosmotic conditions, led to cell shrinkage. RVD in turbot hepatocytes appeared also to depend on proteins p38 MAP kinase and tyrosine kinase but not on proteins ERK 1/2. Arachidonic acid and leukotrienes could also be involved since inhibition of synthesis of both these compounds by quinacrine and NDGA, respectively, inhibited the volume regulation. Likewise, Ca2+ has been proved to be an essential messenger as RVD was prevented in absence of Ca2+. Finally, this work provides bases for novel studies on cell volume regulation in marine teleosteans. |
author2 |
Optimisation des régulations physiologiques (ORPHY (EA 4324)) Institut Brestois Santé Agro Matière (IBSAM) Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest) |
format |
Article in Journal/Newspaper |
author |
Ollivier, Hélène Pichavant, Karine Puill-Stephan, Eneour Roy, Stella Calvès, Patrick Nonnotte, Liliane Nonnotte, Guy |
author_facet |
Ollivier, Hélène Pichavant, Karine Puill-Stephan, Eneour Roy, Stella Calvès, Patrick Nonnotte, Liliane Nonnotte, Guy |
author_sort |
Ollivier, Hélène |
title |
Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes. |
title_short |
Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes. |
title_full |
Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes. |
title_fullStr |
Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes. |
title_full_unstemmed |
Volume regulation following hyposmotic shock in isolated turbot (Scophthalmus maximus) hepatocytes. |
title_sort |
volume regulation following hyposmotic shock in isolated turbot (scophthalmus maximus) hepatocytes. |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.univ-brest.fr/hal-00750437 https://doi.org/10.1007/s00360-005-0061-8 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
ISSN: 0174-1578 EISSN: 1432-136X Journal of Comparative Physiology B https://hal.univ-brest.fr/hal-00750437 Journal of Comparative Physiology B, Springer Verlag, 2006, 176 (5), pp.393-403. ⟨10.1007/s00360-005-0061-8⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00360-005-0061-8 info:eu-repo/semantics/altIdentifier/pmid/16395590 hal-00750437 https://hal.univ-brest.fr/hal-00750437 doi:10.1007/s00360-005-0061-8 PUBMED: 16395590 |
op_doi |
https://doi.org/10.1007/s00360-005-0061-8 |
container_title |
Journal of Comparative Physiology B |
container_volume |
176 |
container_issue |
5 |
container_start_page |
393 |
op_container_end_page |
403 |
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1766188929624571904 |