Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. Interlaboratory validation study
International audience Thirteen laboratories participated in an interlaboratory study to evaluate the method performance characteristics of a liquid chromatography tandem mass spectrometric method (LC-MS/MS) for marine lipophilic shellfish toxins. Method performance characteristics were evaluated fo...
Published in: | Food Additives & Contaminants: Part A |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hal.science/hal-00741567 https://hal.science/hal-00741567/document https://hal.science/hal-00741567/file/PEER_stage2_10.1080%252F19440049.2011.608382.pdf https://doi.org/10.1080/19440049.2011.608382 |
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ftccsdartic:oai:HAL:hal-00741567v1 2023-07-23T04:18:56+02:00 Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. Interlaboratory validation study van den Top, Hester J. Gerssen, Arjen Mccarron, Pearse van Egmond, Hans RIKILT Institute of Food Safety, Wageningen UR Institute for Marine Biosciences National Research Council of Italy RIKILT Institute of Food Safety, Wageningen UR, 2011-10-14 https://hal.science/hal-00741567 https://hal.science/hal-00741567/document https://hal.science/hal-00741567/file/PEER_stage2_10.1080%252F19440049.2011.608382.pdf https://doi.org/10.1080/19440049.2011.608382 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1080/19440049.2011.608382 hal-00741567 https://hal.science/hal-00741567 https://hal.science/hal-00741567/document https://hal.science/hal-00741567/file/PEER_stage2_10.1080%252F19440049.2011.608382.pdf doi:10.1080/19440049.2011.608382 info:eu-repo/semantics/OpenAccess Food Additives and Contaminants https://hal.science/hal-00741567 Food Additives and Contaminants, 2011, pp.1. ⟨10.1080/19440049.2011.608382⟩ Life Sciences info:eu-repo/semantics/article Journal articles 2011 ftccsdartic https://doi.org/10.1080/19440049.2011.608382 2023-07-01T22:35:58Z International audience Thirteen laboratories participated in an interlaboratory study to evaluate the method performance characteristics of a liquid chromatography tandem mass spectrometric method (LC-MS/MS) for marine lipophilic shellfish toxins. Method performance characteristics were evaluated for mussel (Mytilus edulis), oyster (Crassostrea gigas) and cockle (Cerastoderma edule) matrices. The specific toxin analogues tested included okadaic acid (OA), dinophysistoxins-1 and -2 (DTX1,-2), azaspiracids-1, -2 and -3 (AZA1,-2,-3), pectenotoxin-2 (PTX2), yessotoxin (YTX) and 45-OH-yessotoxin (45-OH-YTX). The instrumental technique was developed as an alternative to the still widely applied biological methods (mouse or rat bioassay). Validation was done according to the AOAC harmonised protocol for the design, conduct and interpretation of method-performance studies. Eight different test materials were sent as blind duplicates to the participating laboratories. Twelve laboratories returned results that were accepted to be included in the statistical evaluation. The method precision was expressed as HORRATs. For the individual toxins (except for 45-OH-YTX) HORRATs were found to be ≤ 1.8 (median HORRAT: 0.8) in all tested materials. The recoveries of OA-, AZA- and YTX- group toxins were within the range of 80 - 108% and for PTX2 was within the range of 62 - 93%. Based on the acceptable values for precision and recovery, it was concluded that the method is suitable for official control purposes to quantitatively determine OA/DTXs, AZAs, YTXs and PTX2 in shellfish. Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Food Additives & Contaminants: Part A 1 13 |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Life Sciences |
spellingShingle |
Life Sciences van den Top, Hester J. Gerssen, Arjen Mccarron, Pearse van Egmond, Hans Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. Interlaboratory validation study |
topic_facet |
Life Sciences |
description |
International audience Thirteen laboratories participated in an interlaboratory study to evaluate the method performance characteristics of a liquid chromatography tandem mass spectrometric method (LC-MS/MS) for marine lipophilic shellfish toxins. Method performance characteristics were evaluated for mussel (Mytilus edulis), oyster (Crassostrea gigas) and cockle (Cerastoderma edule) matrices. The specific toxin analogues tested included okadaic acid (OA), dinophysistoxins-1 and -2 (DTX1,-2), azaspiracids-1, -2 and -3 (AZA1,-2,-3), pectenotoxin-2 (PTX2), yessotoxin (YTX) and 45-OH-yessotoxin (45-OH-YTX). The instrumental technique was developed as an alternative to the still widely applied biological methods (mouse or rat bioassay). Validation was done according to the AOAC harmonised protocol for the design, conduct and interpretation of method-performance studies. Eight different test materials were sent as blind duplicates to the participating laboratories. Twelve laboratories returned results that were accepted to be included in the statistical evaluation. The method precision was expressed as HORRATs. For the individual toxins (except for 45-OH-YTX) HORRATs were found to be ≤ 1.8 (median HORRAT: 0.8) in all tested materials. The recoveries of OA-, AZA- and YTX- group toxins were within the range of 80 - 108% and for PTX2 was within the range of 62 - 93%. Based on the acceptable values for precision and recovery, it was concluded that the method is suitable for official control purposes to quantitatively determine OA/DTXs, AZAs, YTXs and PTX2 in shellfish. |
author2 |
RIKILT Institute of Food Safety, Wageningen UR Institute for Marine Biosciences National Research Council of Italy RIKILT Institute of Food Safety, Wageningen UR, |
format |
Article in Journal/Newspaper |
author |
van den Top, Hester J. Gerssen, Arjen Mccarron, Pearse van Egmond, Hans |
author_facet |
van den Top, Hester J. Gerssen, Arjen Mccarron, Pearse van Egmond, Hans |
author_sort |
van den Top, Hester J. |
title |
Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. Interlaboratory validation study |
title_short |
Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. Interlaboratory validation study |
title_full |
Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. Interlaboratory validation study |
title_fullStr |
Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. Interlaboratory validation study |
title_full_unstemmed |
Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. Interlaboratory validation study |
title_sort |
quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography - mass spectrometry. interlaboratory validation study |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.science/hal-00741567 https://hal.science/hal-00741567/document https://hal.science/hal-00741567/file/PEER_stage2_10.1080%252F19440049.2011.608382.pdf https://doi.org/10.1080/19440049.2011.608382 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Food Additives and Contaminants https://hal.science/hal-00741567 Food Additives and Contaminants, 2011, pp.1. ⟨10.1080/19440049.2011.608382⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1080/19440049.2011.608382 hal-00741567 https://hal.science/hal-00741567 https://hal.science/hal-00741567/document https://hal.science/hal-00741567/file/PEER_stage2_10.1080%252F19440049.2011.608382.pdf doi:10.1080/19440049.2011.608382 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1080/19440049.2011.608382 |
container_title |
Food Additives & Contaminants: Part A |
container_start_page |
1 |
op_container_end_page |
13 |
_version_ |
1772181658045251584 |