ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus
Some investigators have proposed the measurement of aspartate transcarbamylase (ATCase) activity as a suitable technique for estimating zooplankton productivity. However, this technique has never been comprehensively evaluated under controlled laboratory conditions nor compared extensively with othe...
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ftccsdartic:oai:HAL:hal-00726589v1 2024-02-11T10:06:41+01:00 ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus Biegala, Isabelle C. Harris, R. P. Bergeron, J. P. Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) University of Texas at Austin Austin 1999-10 https://hal.science/hal-00726589 https://doi.org/10.1007/s002270050595 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s002270050595 hal-00726589 https://hal.science/hal-00726589 doi:10.1007/s002270050595 ISSN: 0025-3162 EISSN: 1432-1793 Marine Biology https://hal.science/hal-00726589 Marine Biology, 1999, 135 (1), pp.1-10. ⟨10.1007/s002270050595⟩ MAXIMAL ENZYME-ACTIVITIES EASTERN NORTH-ATLANTIC SECONDARY PRODUCTION GROWTH-RATE ASPARTATE-TRANSCARBAMYLASE PLANKTONIC COPEPOD PACIFICUS BRODSKY C-HELGOLANDICUS NUCLEIC-ACIDS FINMARCHICUS [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [INFO.INFO-BT]Computer Science [cs]/Biotechnology info:eu-repo/semantics/article Journal articles 1999 ftccsdartic https://doi.org/10.1007/s002270050595 2024-01-27T23:37:57Z Some investigators have proposed the measurement of aspartate transcarbamylase (ATCase) activity as a suitable technique for estimating zooplankton productivity. However, this technique has never been comprehensively evaluated under controlled laboratory conditions nor compared extensively with other productivity techniques in the field. This paper describes such an evaluation, using the reproduction of the calanoid copepod Calanus helgolandicus as an index of germinal growth, an important aspect of zooplankton productivity. ATCase activity and RNA:DNA ratio both increased significantly with increasing gonad maturation. In addition, ATCase activity in females responded immediately to changing food conditions, whilst changes in egg production lagged behind by similar or equal to 24 h. Shifting the ATCase data by 24 h revealed a significant and positive correlation of ATCase activity with egg production. However, in well-fed females, ATCase activity assayed at constant temperature was apparently independent of environmental temperature conditions, whilst egg production was temperature-dependent. In the field, ATCase activity was significantly correlated with egg production only in autumn and winter; when a complete seasonal cycle was considered, no correlation was found between the two measurements. These findings suggest that ATCase is involved partially in either germinal growth and/or in other biosynthetic processes of female C. helgolandicus; thus, ATCase activity does not reflect copepod egg-production with any certainty. Article in Journal/Newspaper North Atlantic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Marine Biology 135 1 1 10 |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
MAXIMAL ENZYME-ACTIVITIES EASTERN NORTH-ATLANTIC SECONDARY PRODUCTION GROWTH-RATE ASPARTATE-TRANSCARBAMYLASE PLANKTONIC COPEPOD PACIFICUS BRODSKY C-HELGOLANDICUS NUCLEIC-ACIDS FINMARCHICUS [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [INFO.INFO-BT]Computer Science [cs]/Biotechnology |
spellingShingle |
MAXIMAL ENZYME-ACTIVITIES EASTERN NORTH-ATLANTIC SECONDARY PRODUCTION GROWTH-RATE ASPARTATE-TRANSCARBAMYLASE PLANKTONIC COPEPOD PACIFICUS BRODSKY C-HELGOLANDICUS NUCLEIC-ACIDS FINMARCHICUS [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [INFO.INFO-BT]Computer Science [cs]/Biotechnology Biegala, Isabelle C. Harris, R. P. Bergeron, J. P. ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus |
topic_facet |
MAXIMAL ENZYME-ACTIVITIES EASTERN NORTH-ATLANTIC SECONDARY PRODUCTION GROWTH-RATE ASPARTATE-TRANSCARBAMYLASE PLANKTONIC COPEPOD PACIFICUS BRODSKY C-HELGOLANDICUS NUCLEIC-ACIDS FINMARCHICUS [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [INFO.INFO-BT]Computer Science [cs]/Biotechnology |
description |
Some investigators have proposed the measurement of aspartate transcarbamylase (ATCase) activity as a suitable technique for estimating zooplankton productivity. However, this technique has never been comprehensively evaluated under controlled laboratory conditions nor compared extensively with other productivity techniques in the field. This paper describes such an evaluation, using the reproduction of the calanoid copepod Calanus helgolandicus as an index of germinal growth, an important aspect of zooplankton productivity. ATCase activity and RNA:DNA ratio both increased significantly with increasing gonad maturation. In addition, ATCase activity in females responded immediately to changing food conditions, whilst changes in egg production lagged behind by similar or equal to 24 h. Shifting the ATCase data by 24 h revealed a significant and positive correlation of ATCase activity with egg production. However, in well-fed females, ATCase activity assayed at constant temperature was apparently independent of environmental temperature conditions, whilst egg production was temperature-dependent. In the field, ATCase activity was significantly correlated with egg production only in autumn and winter; when a complete seasonal cycle was considered, no correlation was found between the two measurements. These findings suggest that ATCase is involved partially in either germinal growth and/or in other biosynthetic processes of female C. helgolandicus; thus, ATCase activity does not reflect copepod egg-production with any certainty. |
author2 |
Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) University of Texas at Austin Austin |
format |
Article in Journal/Newspaper |
author |
Biegala, Isabelle C. Harris, R. P. Bergeron, J. P. |
author_facet |
Biegala, Isabelle C. Harris, R. P. Bergeron, J. P. |
author_sort |
Biegala, Isabelle C. |
title |
ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus |
title_short |
ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus |
title_full |
ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus |
title_fullStr |
ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus |
title_full_unstemmed |
ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus |
title_sort |
atcase activity, rna:dna ratio, gonad development stage, and egg production in the female copepod calanus helgolandicus |
publisher |
HAL CCSD |
publishDate |
1999 |
url |
https://hal.science/hal-00726589 https://doi.org/10.1007/s002270050595 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 0025-3162 EISSN: 1432-1793 Marine Biology https://hal.science/hal-00726589 Marine Biology, 1999, 135 (1), pp.1-10. ⟨10.1007/s002270050595⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s002270050595 hal-00726589 https://hal.science/hal-00726589 doi:10.1007/s002270050595 |
op_doi |
https://doi.org/10.1007/s002270050595 |
container_title |
Marine Biology |
container_volume |
135 |
container_issue |
1 |
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1 |
op_container_end_page |
10 |
_version_ |
1790604576099926016 |