Zinc availability and alkaline phosphatase activity in Emiliania huxleyi: Implications for Zn-P co-limitation in the ocean
International audience Zinc (Zn) serves as a cofactor in several extracellular phosphatases, which allow microorganisms to acquire phosphorus from organic P compounds. In oligotrophic ocean water, where both phosphate and Zn concentrations are low, orthophosphate regeneration through enzymatic hydro...
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ftccsdartic:oai:HAL:hal-00702795v1 2023-05-15T15:43:51+02:00 Zinc availability and alkaline phosphatase activity in Emiliania huxleyi: Implications for Zn-P co-limitation in the ocean Shaked, Y. Xu, Y. Leblanc, Karine Morel, F.M. Department of Geosciences Princeton Princeton University Department of Ecology and Evolutionary Biology Princeton College of Marine Studies (CMS) University of Delaware Newark 2005-09-09 https://hal.archives-ouvertes.fr/hal-00702795 https://doi.org/10.4319/lo.2006.51.1.0299 en eng HAL CCSD Association for the Sciences of Limnology and Oceanography info:eu-repo/semantics/altIdentifier/doi/10.4319/lo.2006.51.1.0299 hal-00702795 https://hal.archives-ouvertes.fr/hal-00702795 doi:10.4319/lo.2006.51.1.0299 ISSN: 0024-3590 EISSN: 1939-5590 Limnology and Oceanography https://hal.archives-ouvertes.fr/hal-00702795 Limnology and Oceanography, Association for the Sciences of Limnology and Oceanography, 2005, 51 (1), pp.299-309. ⟨10.4319/lo.2006.51.1.0299⟩ [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2005 ftccsdartic https://doi.org/10.4319/lo.2006.51.1.0299 2021-11-21T04:07:55Z International audience Zinc (Zn) serves as a cofactor in several extracellular phosphatases, which allow microorganisms to acquire phosphorus from organic P compounds. In oligotrophic ocean water, where both phosphate and Zn concentrations are low, orthophosphate regeneration through enzymatic hydrolysis of organic compounds may be restricted by Zn availability. We examined the possibility of co-limitation by P and Zn in batch cultures of the coccolithophore Emiliania huxleyi grown at very low biomass. Both growth rates and extracellular phosphatase activity were inhibited by low Zn. Cultures grown at nanomolar P and subpicomolar, unchelated Zn concentrations had higher phosphatase activity and slower growth rates when grown on organic P than when grown on inorganic P. We calculated that the additional Zn demand for phosphatase activity in the culture with organic P amounted to 16% of the cellular Zn quota. This percentage would be lower at higher organic P concentrations. Extrapolating from our data, we surmise that Zn-P co-limitation may prevail in highly oligotrophic systems such as the Sargasso Sea, but it is not likely to be widespread in the ocean. Nonetheless, the observation of a significant enhancement of extracellular phosphatase activity in Zn-amended water samples from the Bering Sea demonstrates the potential for Zn-P colimitation during phytoplankton blooms. Article in Journal/Newspaper Bering Sea Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Bering Sea Limnology and Oceanography 51 1 299 309 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Shaked, Y. Xu, Y. Leblanc, Karine Morel, F.M. Zinc availability and alkaline phosphatase activity in Emiliania huxleyi: Implications for Zn-P co-limitation in the ocean |
topic_facet |
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience Zinc (Zn) serves as a cofactor in several extracellular phosphatases, which allow microorganisms to acquire phosphorus from organic P compounds. In oligotrophic ocean water, where both phosphate and Zn concentrations are low, orthophosphate regeneration through enzymatic hydrolysis of organic compounds may be restricted by Zn availability. We examined the possibility of co-limitation by P and Zn in batch cultures of the coccolithophore Emiliania huxleyi grown at very low biomass. Both growth rates and extracellular phosphatase activity were inhibited by low Zn. Cultures grown at nanomolar P and subpicomolar, unchelated Zn concentrations had higher phosphatase activity and slower growth rates when grown on organic P than when grown on inorganic P. We calculated that the additional Zn demand for phosphatase activity in the culture with organic P amounted to 16% of the cellular Zn quota. This percentage would be lower at higher organic P concentrations. Extrapolating from our data, we surmise that Zn-P co-limitation may prevail in highly oligotrophic systems such as the Sargasso Sea, but it is not likely to be widespread in the ocean. Nonetheless, the observation of a significant enhancement of extracellular phosphatase activity in Zn-amended water samples from the Bering Sea demonstrates the potential for Zn-P colimitation during phytoplankton blooms. |
author2 |
Department of Geosciences Princeton Princeton University Department of Ecology and Evolutionary Biology Princeton College of Marine Studies (CMS) University of Delaware Newark |
format |
Article in Journal/Newspaper |
author |
Shaked, Y. Xu, Y. Leblanc, Karine Morel, F.M. |
author_facet |
Shaked, Y. Xu, Y. Leblanc, Karine Morel, F.M. |
author_sort |
Shaked, Y. |
title |
Zinc availability and alkaline phosphatase activity in Emiliania huxleyi: Implications for Zn-P co-limitation in the ocean |
title_short |
Zinc availability and alkaline phosphatase activity in Emiliania huxleyi: Implications for Zn-P co-limitation in the ocean |
title_full |
Zinc availability and alkaline phosphatase activity in Emiliania huxleyi: Implications for Zn-P co-limitation in the ocean |
title_fullStr |
Zinc availability and alkaline phosphatase activity in Emiliania huxleyi: Implications for Zn-P co-limitation in the ocean |
title_full_unstemmed |
Zinc availability and alkaline phosphatase activity in Emiliania huxleyi: Implications for Zn-P co-limitation in the ocean |
title_sort |
zinc availability and alkaline phosphatase activity in emiliania huxleyi: implications for zn-p co-limitation in the ocean |
publisher |
HAL CCSD |
publishDate |
2005 |
url |
https://hal.archives-ouvertes.fr/hal-00702795 https://doi.org/10.4319/lo.2006.51.1.0299 |
geographic |
Bering Sea |
geographic_facet |
Bering Sea |
genre |
Bering Sea |
genre_facet |
Bering Sea |
op_source |
ISSN: 0024-3590 EISSN: 1939-5590 Limnology and Oceanography https://hal.archives-ouvertes.fr/hal-00702795 Limnology and Oceanography, Association for the Sciences of Limnology and Oceanography, 2005, 51 (1), pp.299-309. ⟨10.4319/lo.2006.51.1.0299⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.4319/lo.2006.51.1.0299 hal-00702795 https://hal.archives-ouvertes.fr/hal-00702795 doi:10.4319/lo.2006.51.1.0299 |
op_doi |
https://doi.org/10.4319/lo.2006.51.1.0299 |
container_title |
Limnology and Oceanography |
container_volume |
51 |
container_issue |
1 |
container_start_page |
299 |
op_container_end_page |
309 |
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1766378056591605760 |