Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae
International audience Parameters used to assess the survival of larvae after cryopreservation generally misestimate the damages that prevent larval development. The objectives of the present study were to 1) define the reliability of the survival rate, assessed at 2 and 7 days post fertilization, t...
Published in: | Theriogenology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2012
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Online Access: | https://hal.univ-brest.fr/hal-00693177 https://doi.org/10.1016/j.theriogenology.2012.02.014 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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English |
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CASA ASMA Crassostrea gigas Cryopreservation D-larvae [SDE]Environmental Sciences |
spellingShingle |
CASA ASMA Crassostrea gigas Cryopreservation D-larvae [SDE]Environmental Sciences Suquet, Marc Le Mercier, Alain Rimond, F. Mingant, C. Haffray, Pierrick Labbé, Catherine Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae |
topic_facet |
CASA ASMA Crassostrea gigas Cryopreservation D-larvae [SDE]Environmental Sciences |
description |
International audience Parameters used to assess the survival of larvae after cryopreservation generally misestimate the damages that prevent larval development. The objectives of the present study were to 1) define the reliability of the survival rate, assessed at 2 and 7 days post fertilization, to estimate Pacific oyster larval quality after thawing, and 2) select complementary tools allowing an early and reliable estimation of their quality. Oyster larvae were reared for 25 h after fertilization at 19 °C and cryopreserved at early D-stage. Then, thawed larvae were incubated in 2-L beakers. At 2 days post fertilization, the survival rate of thawed Pacific oyster larvae was lower than that of fresh larvae for only one experiment (Experiment 3) among the four identical experiments carried out in this work (Experiments 1-4). By contrast, the survival of thawed larvae, as assessed 7 days after fertilization, was lower than that of fresh larvae for the four experiments. These results confirm that the quality of thawed larvae is lower than that of fresh larvae and that the survival rate, estimated 2 days post fertilization, is not adapted to a reliable estimation of the subsequent development ability of thawed larvae. Then, complementary parameters were tested at 2 days: the movement characteristics (Experiments 1 and 2) and the morphologic features (Experiments 3 and 4) of thawed larvae. Compared to values observed on fresh larvae, the percentage of thawed motile larvae was different for only one experiment (Experiment 2) of the two. Compared to control, a reduced Average Path Velocity (VAP) of larvae (determined at the D-larval stage using a CASA-Computer Assisted Sperm Analysis-system) was observed after thawing for both experiments (Experiments 1 and 2), suggesting the ability of larval movement velocity to assess the decrease of the quality of thawed oyster larvae. Using an ASMA (Automated Sperm Morphology Analysis) device, a lower area of thawed larvae was observed, compared to control and for the two ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) IFREMER (DPFOM LPI) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF) Laboratoire de Physiologie et Génomique des Poissons (LPGP) Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) CRECHE (Ofimer 136/08/C) - CRYOAQUA (GIS ibisa) - European Union (FEP 30906-2009) |
format |
Article in Journal/Newspaper |
author |
Suquet, Marc Le Mercier, Alain Rimond, F. Mingant, C. Haffray, Pierrick Labbé, Catherine |
author_facet |
Suquet, Marc Le Mercier, Alain Rimond, F. Mingant, C. Haffray, Pierrick Labbé, Catherine |
author_sort |
Suquet, Marc |
title |
Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae |
title_short |
Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae |
title_full |
Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae |
title_fullStr |
Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae |
title_full_unstemmed |
Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae |
title_sort |
setting tools for the early assessment of the quality of thawed pacific oyster (crassostrea gigas) d-larvae |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00693177 https://doi.org/10.1016/j.theriogenology.2012.02.014 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0093-691X Theriogenology https://hal.univ-brest.fr/hal-00693177 Theriogenology, Elsevier, 2012, 78 (2), pp.462-467. ⟨10.1016/j.theriogenology.2012.02.014⟩ |
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2012.02.014 hal-00693177 https://hal.univ-brest.fr/hal-00693177 doi:10.1016/j.theriogenology.2012.02.014 PRODINRA: 171032 WOS: 000306039600025 |
op_doi |
https://doi.org/10.1016/j.theriogenology.2012.02.014 |
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Theriogenology |
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467 |
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ftccsdartic:oai:HAL:hal-00693177v1 2023-05-15T15:58:31+02:00 Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae Suquet, Marc Le Mercier, Alain Rimond, F. Mingant, C. Haffray, Pierrick Labbé, Catherine Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) IFREMER (DPFOM LPI) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF) Laboratoire de Physiologie et Génomique des Poissons (LPGP) Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) CRECHE (Ofimer 136/08/C) - CRYOAQUA (GIS ibisa) - European Union (FEP 30906-2009) 2012-05-02 https://hal.univ-brest.fr/hal-00693177 https://doi.org/10.1016/j.theriogenology.2012.02.014 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2012.02.014 hal-00693177 https://hal.univ-brest.fr/hal-00693177 doi:10.1016/j.theriogenology.2012.02.014 PRODINRA: 171032 WOS: 000306039600025 ISSN: 0093-691X Theriogenology https://hal.univ-brest.fr/hal-00693177 Theriogenology, Elsevier, 2012, 78 (2), pp.462-467. ⟨10.1016/j.theriogenology.2012.02.014⟩ CASA ASMA Crassostrea gigas Cryopreservation D-larvae [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftccsdartic https://doi.org/10.1016/j.theriogenology.2012.02.014 2021-12-19T03:27:33Z International audience Parameters used to assess the survival of larvae after cryopreservation generally misestimate the damages that prevent larval development. The objectives of the present study were to 1) define the reliability of the survival rate, assessed at 2 and 7 days post fertilization, to estimate Pacific oyster larval quality after thawing, and 2) select complementary tools allowing an early and reliable estimation of their quality. Oyster larvae were reared for 25 h after fertilization at 19 °C and cryopreserved at early D-stage. Then, thawed larvae were incubated in 2-L beakers. At 2 days post fertilization, the survival rate of thawed Pacific oyster larvae was lower than that of fresh larvae for only one experiment (Experiment 3) among the four identical experiments carried out in this work (Experiments 1-4). By contrast, the survival of thawed larvae, as assessed 7 days after fertilization, was lower than that of fresh larvae for the four experiments. These results confirm that the quality of thawed larvae is lower than that of fresh larvae and that the survival rate, estimated 2 days post fertilization, is not adapted to a reliable estimation of the subsequent development ability of thawed larvae. Then, complementary parameters were tested at 2 days: the movement characteristics (Experiments 1 and 2) and the morphologic features (Experiments 3 and 4) of thawed larvae. Compared to values observed on fresh larvae, the percentage of thawed motile larvae was different for only one experiment (Experiment 2) of the two. Compared to control, a reduced Average Path Velocity (VAP) of larvae (determined at the D-larval stage using a CASA-Computer Assisted Sperm Analysis-system) was observed after thawing for both experiments (Experiments 1 and 2), suggesting the ability of larval movement velocity to assess the decrease of the quality of thawed oyster larvae. Using an ASMA (Automated Sperm Morphology Analysis) device, a lower area of thawed larvae was observed, compared to control and for the two ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Theriogenology 78 2 462 467 |