CO2 and vitamin B-12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom
Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B-12 and carbon dioxide partial pressure (pCO(2)) influences growth rate, primary productivity, cellu...
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ftccsdartic:oai:HAL:hal-00687942v1 2023-12-17T10:48:06+01:00 CO2 and vitamin B-12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom King, Andrew L. Sanudo-Wilhelmy, Sergio A. Leblanc, Karine Hutchins, David A. Fu, Feixue Institut méditerranéen d'océanologie (MIO) Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS) 2011 https://hal.science/hal-00687942 https://doi.org/10.1038/ismej.2010.211 en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/ismej.2010.211 hal-00687942 https://hal.science/hal-00687942 doi:10.1038/ismej.2010.211 PUBMEDCENTRAL: PMC3146264 ISSN: 1751-7362 EISSN: 1751-7370 ISME Journal https://hal.science/hal-00687942 ISME Journal, 2011, 5, pp.1388-1396. ⟨10.1038/ismej.2010.211⟩ phytoplankton vitamin B-12 carbon dioxide ocean acidification trace metals MARINE-PHYTOPLANKTON THALASSIOSIRA-WEISSFLOGII GROWTH LIMITATION IRON COASTAL OCEAN ZINC TEMPERATURE BACTERIA CADMIUM [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere info:eu-repo/semantics/article Journal articles 2011 ftccsdartic https://doi.org/10.1038/ismej.2010.211 2023-11-18T23:29:43Z Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B-12 and carbon dioxide partial pressure (pCO(2)) influences growth rate, primary productivity, cellular iron (Fe), cobalt (Co), zinc (Zn) and cadmium (Cd) quotas, and the net use efficiencies (NUEs) of these bioactive trace metals (mol C fixed per mol cellular trace metal per day). Under B-12-replete conditions, cells grown at high pCO(2) had lower Fe, Zn and Cd quotas, and used those trace metals more efficiently in comparison with cells grown at low pCO(2). At high pCO(2), B-12-limited cells had similar to 50% lower specific growth and carbon fixation rates, and used Fe similar to 15-fold less efficiently, and Zn and Cd similar to 3-fold less efficiently, in comparison with B-12-replete cells. The observed higher Fe, Zn and Cd NUE under high pCO(2)/B-12-replete conditions are consistent with predicted downregulation of carbon-concentrating mechanisms. Co quotas of B-12-replete cells were similar to 5- to 14-fold higher in comparison with B-12-limited cells, suggesting that >80% of cellular Co of B-12-limited cells was likely from B-12. Our results demonstrate that CO2 and vitamin B-12 interactively influence growth, carbon fixation, trace metal requirements and trace metal NUE of this diatom. This suggests the need to consider complex feedback interactions between multiple environmental factors for this biogeochemically critical group of phytoplankton in the last glacial maximum as well as the current and future changing ocean. The ISME Journal (2011) 5, 1388-1396; doi:10.1038/ismej.2010.211; published online 20 January 2011 Article in Journal/Newspaper Ocean acidification Subarctic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific The ISME Journal 5 8 1388 1396 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
phytoplankton vitamin B-12 carbon dioxide ocean acidification trace metals MARINE-PHYTOPLANKTON THALASSIOSIRA-WEISSFLOGII GROWTH LIMITATION IRON COASTAL OCEAN ZINC TEMPERATURE BACTERIA CADMIUM [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere |
spellingShingle |
phytoplankton vitamin B-12 carbon dioxide ocean acidification trace metals MARINE-PHYTOPLANKTON THALASSIOSIRA-WEISSFLOGII GROWTH LIMITATION IRON COASTAL OCEAN ZINC TEMPERATURE BACTERIA CADMIUM [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere King, Andrew L. Sanudo-Wilhelmy, Sergio A. Leblanc, Karine Hutchins, David A. Fu, Feixue CO2 and vitamin B-12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
topic_facet |
phytoplankton vitamin B-12 carbon dioxide ocean acidification trace metals MARINE-PHYTOPLANKTON THALASSIOSIRA-WEISSFLOGII GROWTH LIMITATION IRON COASTAL OCEAN ZINC TEMPERATURE BACTERIA CADMIUM [SDU.OCEAN]Sciences of the Universe [physics]/Ocean Atmosphere |
description |
Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B-12 and carbon dioxide partial pressure (pCO(2)) influences growth rate, primary productivity, cellular iron (Fe), cobalt (Co), zinc (Zn) and cadmium (Cd) quotas, and the net use efficiencies (NUEs) of these bioactive trace metals (mol C fixed per mol cellular trace metal per day). Under B-12-replete conditions, cells grown at high pCO(2) had lower Fe, Zn and Cd quotas, and used those trace metals more efficiently in comparison with cells grown at low pCO(2). At high pCO(2), B-12-limited cells had similar to 50% lower specific growth and carbon fixation rates, and used Fe similar to 15-fold less efficiently, and Zn and Cd similar to 3-fold less efficiently, in comparison with B-12-replete cells. The observed higher Fe, Zn and Cd NUE under high pCO(2)/B-12-replete conditions are consistent with predicted downregulation of carbon-concentrating mechanisms. Co quotas of B-12-replete cells were similar to 5- to 14-fold higher in comparison with B-12-limited cells, suggesting that >80% of cellular Co of B-12-limited cells was likely from B-12. Our results demonstrate that CO2 and vitamin B-12 interactively influence growth, carbon fixation, trace metal requirements and trace metal NUE of this diatom. This suggests the need to consider complex feedback interactions between multiple environmental factors for this biogeochemically critical group of phytoplankton in the last glacial maximum as well as the current and future changing ocean. The ISME Journal (2011) 5, 1388-1396; doi:10.1038/ismej.2010.211; published online 20 January 2011 |
author2 |
Institut méditerranéen d'océanologie (MIO) Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
King, Andrew L. Sanudo-Wilhelmy, Sergio A. Leblanc, Karine Hutchins, David A. Fu, Feixue |
author_facet |
King, Andrew L. Sanudo-Wilhelmy, Sergio A. Leblanc, Karine Hutchins, David A. Fu, Feixue |
author_sort |
King, Andrew L. |
title |
CO2 and vitamin B-12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_short |
CO2 and vitamin B-12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_full |
CO2 and vitamin B-12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_fullStr |
CO2 and vitamin B-12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_full_unstemmed |
CO2 and vitamin B-12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_sort |
co2 and vitamin b-12 interactions determine bioactive trace metal requirements of a subarctic pacific diatom |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.science/hal-00687942 https://doi.org/10.1038/ismej.2010.211 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification Subarctic |
genre_facet |
Ocean acidification Subarctic |
op_source |
ISSN: 1751-7362 EISSN: 1751-7370 ISME Journal https://hal.science/hal-00687942 ISME Journal, 2011, 5, pp.1388-1396. ⟨10.1038/ismej.2010.211⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/ismej.2010.211 hal-00687942 https://hal.science/hal-00687942 doi:10.1038/ismej.2010.211 PUBMEDCENTRAL: PMC3146264 |
op_doi |
https://doi.org/10.1038/ismej.2010.211 |
container_title |
The ISME Journal |
container_volume |
5 |
container_issue |
8 |
container_start_page |
1388 |
op_container_end_page |
1396 |
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1785572183136796672 |