Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat
International audience In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially...
Published in: | Chemosphere |
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Online Access: | https://hal.archives-ouvertes.fr/hal-00686855 https://hal.archives-ouvertes.fr/hal-00686855/document https://hal.archives-ouvertes.fr/hal-00686855/file/Luna-Acosta_et_al_2012_CHEM.pdf https://doi.org/10.1016/j.chemosphere.2012.02.022 |
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ftccsdartic:oai:HAL:hal-00686855v1 2023-05-15T15:58:10+02:00 Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat Luna-Acosta, Andrea Renault, Tristan Thomas-Guyon, Hélène Faury, Nicole Saulnier, Denis Budzinski, Hélène Le Ménach, Karine Pardon, P. Fruitier-Arnaudin, Ingrid Bustamante, Paco LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Fédération de Recherche en Environnement pour le Développement Durable (FREDD) Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie (LGP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Physico -& Toxico Chimie des systèmes naturels (LPTC) Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS) 2012-06 https://hal.archives-ouvertes.fr/hal-00686855 https://hal.archives-ouvertes.fr/hal-00686855/document https://hal.archives-ouvertes.fr/hal-00686855/file/Luna-Acosta_et_al_2012_CHEM.pdf https://doi.org/10.1016/j.chemosphere.2012.02.022 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2012.02.022 hal-00686855 https://hal.archives-ouvertes.fr/hal-00686855 https://hal.archives-ouvertes.fr/hal-00686855/document https://hal.archives-ouvertes.fr/hal-00686855/file/Luna-Acosta_et_al_2012_CHEM.pdf doi:10.1016/j.chemosphere.2012.02.022 info:eu-repo/semantics/OpenAccess ISSN: 0045-6535 Chemosphere https://hal.archives-ouvertes.fr/hal-00686855 Chemosphere, Elsevier, 2012, 87 (11), pp.1335-1340. ⟨10.1016/j.chemosphere.2012.02.022⟩ bivalve pollution gene expression laccase superoxide dismutase phagocytosis [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology info:eu-repo/semantics/article Journal articles 2012 ftccsdartic https://doi.org/10.1016/j.chemosphere.2012.02.022 2021-10-24T16:35:32Z International audience In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 hours of exposure to the herbicide diuron at 1 µg L-1, the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 hours of exposure. In the mixture with the herbicides diuron and isoproturon, and the pharmaceutical ibuprofen, catecholase-type PO activity in the plasma and the percentage of phagocytosis in the haemocytes were reduced after 6 hours of exposure. Our results showed that early effects on molecular, biochemical and cellular parameters can be detected in the presence of diuron alone or within a mixture, giving an insight of its potential effect in situations that can be found in natural environments, i.e. relatively high concentrations for short periods of time. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Chemosphere 87 11 1335 1340 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
bivalve pollution gene expression laccase superoxide dismutase phagocytosis [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
spellingShingle |
bivalve pollution gene expression laccase superoxide dismutase phagocytosis [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Luna-Acosta, Andrea Renault, Tristan Thomas-Guyon, Hélène Faury, Nicole Saulnier, Denis Budzinski, Hélène Le Ménach, Karine Pardon, P. Fruitier-Arnaudin, Ingrid Bustamante, Paco Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat |
topic_facet |
bivalve pollution gene expression laccase superoxide dismutase phagocytosis [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
description |
International audience In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 hours of exposure to the herbicide diuron at 1 µg L-1, the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 hours of exposure. In the mixture with the herbicides diuron and isoproturon, and the pharmaceutical ibuprofen, catecholase-type PO activity in the plasma and the percentage of phagocytosis in the haemocytes were reduced after 6 hours of exposure. Our results showed that early effects on molecular, biochemical and cellular parameters can be detected in the presence of diuron alone or within a mixture, giving an insight of its potential effect in situations that can be found in natural environments, i.e. relatively high concentrations for short periods of time. |
author2 |
LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Fédération de Recherche en Environnement pour le Développement Durable (FREDD) Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie (LGP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Physico -& Toxico Chimie des systèmes naturels (LPTC) Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Luna-Acosta, Andrea Renault, Tristan Thomas-Guyon, Hélène Faury, Nicole Saulnier, Denis Budzinski, Hélène Le Ménach, Karine Pardon, P. Fruitier-Arnaudin, Ingrid Bustamante, Paco |
author_facet |
Luna-Acosta, Andrea Renault, Tristan Thomas-Guyon, Hélène Faury, Nicole Saulnier, Denis Budzinski, Hélène Le Ménach, Karine Pardon, P. Fruitier-Arnaudin, Ingrid Bustamante, Paco |
author_sort |
Luna-Acosta, Andrea |
title |
Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat |
title_short |
Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat |
title_full |
Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat |
title_fullStr |
Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat |
title_full_unstemmed |
Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat |
title_sort |
detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of pacific oyster (crassostrea gigas) spat |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.archives-ouvertes.fr/hal-00686855 https://hal.archives-ouvertes.fr/hal-00686855/document https://hal.archives-ouvertes.fr/hal-00686855/file/Luna-Acosta_et_al_2012_CHEM.pdf https://doi.org/10.1016/j.chemosphere.2012.02.022 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0045-6535 Chemosphere https://hal.archives-ouvertes.fr/hal-00686855 Chemosphere, Elsevier, 2012, 87 (11), pp.1335-1340. ⟨10.1016/j.chemosphere.2012.02.022⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2012.02.022 hal-00686855 https://hal.archives-ouvertes.fr/hal-00686855 https://hal.archives-ouvertes.fr/hal-00686855/document https://hal.archives-ouvertes.fr/hal-00686855/file/Luna-Acosta_et_al_2012_CHEM.pdf doi:10.1016/j.chemosphere.2012.02.022 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.chemosphere.2012.02.022 |
container_title |
Chemosphere |
container_volume |
87 |
container_issue |
11 |
container_start_page |
1335 |
op_container_end_page |
1340 |
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1766393893125881856 |