Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009
International audience The emergence of West Nile virus (WNV) was expected in Austria since the initial discovery of the infection in neighbouring Hungary in 2003/2004. In 2008 six cases of West Nile disease were diagnosed at the Institute for Veterinary Disease Control Mödling, Austrian Agency for...
Published in: | Veterinary Microbiology |
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2011
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Online Access: | https://hal.archives-ouvertes.fr/hal-00683508 https://hal.archives-ouvertes.fr/hal-00683508/document https://hal.archives-ouvertes.fr/hal-00683508/file/PEER_stage2_10.1016%252Fj.vetmic.2010.12.012.pdf https://doi.org/10.1016/j.vetmic.2010.12.012 |
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ftccsdartic:oai:HAL:hal-00683508v1 2023-05-15T16:32:26+02:00 Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009 Wodak, Eveline Richter, Susanne Bagó, Zoltán. Revilla-Fernández, Sandra Weissenböck, Herbert Nowotny, Norbert Winter, Petra Austrian Agency for Health and Food Safety (AGES) Department of Pathobiology University of Veterinary Medicine Zoonoses and Emerging Infections Group 2011-03-29 https://hal.archives-ouvertes.fr/hal-00683508 https://hal.archives-ouvertes.fr/hal-00683508/document https://hal.archives-ouvertes.fr/hal-00683508/file/PEER_stage2_10.1016%252Fj.vetmic.2010.12.012.pdf https://doi.org/10.1016/j.vetmic.2010.12.012 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2010.12.012 hal-00683508 https://hal.archives-ouvertes.fr/hal-00683508 https://hal.archives-ouvertes.fr/hal-00683508/document https://hal.archives-ouvertes.fr/hal-00683508/file/PEER_stage2_10.1016%252Fj.vetmic.2010.12.012.pdf doi:10.1016/j.vetmic.2010.12.012 info:eu-repo/semantics/OpenAccess ISSN: 0378-1135 Veterinary Microbiology https://hal.archives-ouvertes.fr/hal-00683508 Veterinary Microbiology, Elsevier, 2011, 149 (3-4), pp.358. ⟨10.1016/j.vetmic.2010.12.012⟩ West Nile virus Flavivirus mosquito-borne flavivirus zoonosis goshawk Austria [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health info:eu-repo/semantics/article Journal articles 2011 ftccsdartic https://doi.org/10.1016/j.vetmic.2010.12.012 2020-12-26T04:54:28Z International audience The emergence of West Nile virus (WNV) was expected in Austria since the initial discovery of the infection in neighbouring Hungary in 2003/2004. In 2008 six cases of West Nile disease were diagnosed at the Institute for Veterinary Disease Control Mödling, Austrian Agency for Health and Food Safety (AGES), involving five goshawks () and one gyrfalcon (), which were found dead in the eastern Austrian federal states of Lower Austria, Vienna and Styria, respectively. Pathomorphological and immunohistochemical findings suggested a WNV infection. Virus was isolated in embryonated specific pathogen free chicken eggs and propagated in mouse neuroblastoma cells (NA), in which a cytopathic effect occurred. The virus was identified and characterised by electron microscopic examination and molecular detection using RT-PCR, sequencing, and phylogenetic analysis. The Austrian WNV sequences exhibited nucleotide identities of 99.9% to the lineage 2 WNV sequences described in Hungary since 2004. In addition, 71 sera of 14 different bird species were screened for the presence of WNV antibodies using a commercial ELISA: 43,7% of the tested samples showed antibody titres. Selected positive sera were also subjected to WNV neutralisation tests, in which the ELISA results were verified in 66%. The results of this study confirm unambiguously the presence of a lineage 2 WNV infection in birds of prey in the eastern part of Austria. Article in Journal/Newspaper gyrfalcon Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Veterinary Microbiology 149 3-4 358 366 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
West Nile virus Flavivirus mosquito-borne flavivirus zoonosis goshawk Austria [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health |
spellingShingle |
West Nile virus Flavivirus mosquito-borne flavivirus zoonosis goshawk Austria [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Wodak, Eveline Richter, Susanne Bagó, Zoltán. Revilla-Fernández, Sandra Weissenböck, Herbert Nowotny, Norbert Winter, Petra Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009 |
topic_facet |
West Nile virus Flavivirus mosquito-borne flavivirus zoonosis goshawk Austria [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health |
description |
International audience The emergence of West Nile virus (WNV) was expected in Austria since the initial discovery of the infection in neighbouring Hungary in 2003/2004. In 2008 six cases of West Nile disease were diagnosed at the Institute for Veterinary Disease Control Mödling, Austrian Agency for Health and Food Safety (AGES), involving five goshawks () and one gyrfalcon (), which were found dead in the eastern Austrian federal states of Lower Austria, Vienna and Styria, respectively. Pathomorphological and immunohistochemical findings suggested a WNV infection. Virus was isolated in embryonated specific pathogen free chicken eggs and propagated in mouse neuroblastoma cells (NA), in which a cytopathic effect occurred. The virus was identified and characterised by electron microscopic examination and molecular detection using RT-PCR, sequencing, and phylogenetic analysis. The Austrian WNV sequences exhibited nucleotide identities of 99.9% to the lineage 2 WNV sequences described in Hungary since 2004. In addition, 71 sera of 14 different bird species were screened for the presence of WNV antibodies using a commercial ELISA: 43,7% of the tested samples showed antibody titres. Selected positive sera were also subjected to WNV neutralisation tests, in which the ELISA results were verified in 66%. The results of this study confirm unambiguously the presence of a lineage 2 WNV infection in birds of prey in the eastern part of Austria. |
author2 |
Austrian Agency for Health and Food Safety (AGES) Department of Pathobiology University of Veterinary Medicine Zoonoses and Emerging Infections Group |
format |
Article in Journal/Newspaper |
author |
Wodak, Eveline Richter, Susanne Bagó, Zoltán. Revilla-Fernández, Sandra Weissenböck, Herbert Nowotny, Norbert Winter, Petra |
author_facet |
Wodak, Eveline Richter, Susanne Bagó, Zoltán. Revilla-Fernández, Sandra Weissenböck, Herbert Nowotny, Norbert Winter, Petra |
author_sort |
Wodak, Eveline |
title |
Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009 |
title_short |
Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009 |
title_full |
Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009 |
title_fullStr |
Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009 |
title_full_unstemmed |
Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009 |
title_sort |
detection and molecular analysis of west nile virus infections in birds of prey in the eastern part of austria in 2008 and 2009 |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.archives-ouvertes.fr/hal-00683508 https://hal.archives-ouvertes.fr/hal-00683508/document https://hal.archives-ouvertes.fr/hal-00683508/file/PEER_stage2_10.1016%252Fj.vetmic.2010.12.012.pdf https://doi.org/10.1016/j.vetmic.2010.12.012 |
genre |
gyrfalcon |
genre_facet |
gyrfalcon |
op_source |
ISSN: 0378-1135 Veterinary Microbiology https://hal.archives-ouvertes.fr/hal-00683508 Veterinary Microbiology, Elsevier, 2011, 149 (3-4), pp.358. ⟨10.1016/j.vetmic.2010.12.012⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2010.12.012 hal-00683508 https://hal.archives-ouvertes.fr/hal-00683508 https://hal.archives-ouvertes.fr/hal-00683508/document https://hal.archives-ouvertes.fr/hal-00683508/file/PEER_stage2_10.1016%252Fj.vetmic.2010.12.012.pdf doi:10.1016/j.vetmic.2010.12.012 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.vetmic.2010.12.012 |
container_title |
Veterinary Microbiology |
container_volume |
149 |
container_issue |
3-4 |
container_start_page |
358 |
op_container_end_page |
366 |
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1766022175404326912 |