Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas
International audience Pacific oysters, Crassostrea gigas, living at a range of tidal heights, routinely encounter large fluctuations in temperature. We demonstrate that levels of heat shock proteins (HSP) and other stress proteins (metallothioneins, MTs) quantified by ELISA, remained similar in gil...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hal.univ-brest.fr/hal-00670394 https://doi.org/10.1016/j.cbpa.2008.10.004 |
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ftccsdartic:oai:HAL:hal-00670394v1 2023-05-15T15:57:35+02:00 Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas Meistertzheim, Anne-Leila Lejart, Morgane Le Goïc, Nelly Thébault, Marie-Therese Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2009 https://hal.univ-brest.fr/hal-00670394 https://doi.org/10.1016/j.cbpa.2008.10.004 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2008.10.004 hal-00670394 https://hal.univ-brest.fr/hal-00670394 doi:10.1016/j.cbpa.2008.10.004 ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal.univ-brest.fr/hal-00670394 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2009, 152 (2), pp.234-239. ⟨10.1016/j.cbpa.2008.10.004⟩ Crassostrea gigas Reproduction Heat shock proteins Metallothioneins Sex Gonad Maturation stage [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2009 ftccsdartic https://doi.org/10.1016/j.cbpa.2008.10.004 2021-12-19T03:29:34Z International audience Pacific oysters, Crassostrea gigas, living at a range of tidal heights, routinely encounter large fluctuations in temperature. We demonstrate that levels of heat shock proteins (HSP) and other stress proteins (metallothioneins, MTs) quantified by ELISA, remained similar in gills, mantle and digestive gland between oysters inhabiting low and high tidal heights. In contrast, endogenous HSPs and MITs levels in gonad changed significantly during gametogenesis. In female gonads, the constitutive form of HSP70 and the MTs increased from immature (or resting) to mature stages (about more than 3-fold) and decreased after spawning. In male gonads, the same expression patterns were observed, whereas Proteins levels decreased once fully mature. Females presented higher concentration of HSP70 and MTs than males during the spawning period. No significant difference in HSPs and MTs patterns was found among oysters sampled at low and high tidal heights. We hypothesize that the high level of stress proteins in eggs may increase survival of oyster progeny. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 152 2 234 239 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Crassostrea gigas Reproduction Heat shock proteins Metallothioneins Sex Gonad Maturation stage [SDE]Environmental Sciences |
spellingShingle |
Crassostrea gigas Reproduction Heat shock proteins Metallothioneins Sex Gonad Maturation stage [SDE]Environmental Sciences Meistertzheim, Anne-Leila Lejart, Morgane Le Goïc, Nelly Thébault, Marie-Therese Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas Reproduction Heat shock proteins Metallothioneins Sex Gonad Maturation stage [SDE]Environmental Sciences |
description |
International audience Pacific oysters, Crassostrea gigas, living at a range of tidal heights, routinely encounter large fluctuations in temperature. We demonstrate that levels of heat shock proteins (HSP) and other stress proteins (metallothioneins, MTs) quantified by ELISA, remained similar in gills, mantle and digestive gland between oysters inhabiting low and high tidal heights. In contrast, endogenous HSPs and MITs levels in gonad changed significantly during gametogenesis. In female gonads, the constitutive form of HSP70 and the MTs increased from immature (or resting) to mature stages (about more than 3-fold) and decreased after spawning. In male gonads, the same expression patterns were observed, whereas Proteins levels decreased once fully mature. Females presented higher concentration of HSP70 and MTs than males during the spawning period. No significant difference in HSPs and MTs patterns was found among oysters sampled at low and high tidal heights. We hypothesize that the high level of stress proteins in eggs may increase survival of oyster progeny. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Meistertzheim, Anne-Leila Lejart, Morgane Le Goïc, Nelly Thébault, Marie-Therese |
author_facet |
Meistertzheim, Anne-Leila Lejart, Morgane Le Goïc, Nelly Thébault, Marie-Therese |
author_sort |
Meistertzheim, Anne-Leila |
title |
Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas |
title_short |
Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas |
title_full |
Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas |
title_fullStr |
Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas |
title_sort |
sex-, gametogenesis, and tidal height-related differences in levels of hsp70 and metallothioneins in the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.univ-brest.fr/hal-00670394 https://doi.org/10.1016/j.cbpa.2008.10.004 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal.univ-brest.fr/hal-00670394 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2009, 152 (2), pp.234-239. ⟨10.1016/j.cbpa.2008.10.004⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2008.10.004 hal-00670394 https://hal.univ-brest.fr/hal-00670394 doi:10.1016/j.cbpa.2008.10.004 |
op_doi |
https://doi.org/10.1016/j.cbpa.2008.10.004 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
152 |
container_issue |
2 |
container_start_page |
234 |
op_container_end_page |
239 |
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