Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates
International audience Based on the combination of standard light and transmission electron microscopy, cryo-SEM, immunohistochemistry and a new sensitive glycolipid histochemical technique (5-hexadecanoylaminofluorescein staining, laser scanning microscopy), including densitometrical evaluation, ou...
Published in: | European Journal of Wildlife Research |
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Online Access: | https://hal.science/hal-00664099 https://hal.science/hal-00664099/document https://hal.science/hal-00664099/file/10344_2011_499_ReferencePDF.pdf https://doi.org/10.1007/s10344-011-0499-9 |
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ftccsdartic:oai:HAL:hal-00664099v1 2024-02-11T10:02:57+01:00 Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates Meyer, Wilfried Schmidt, Judith Kacza, Johannes Busche, Roger Naim, Hassan Y. Jacob, Ralf Institute for Anatomy University of Veterinary Medicine Hannover, Foundation Institute of Veterinary Anatomy Universität Leipzig Institute for Physiological Chemistry Institute of Cytobiology and Cytopathology Philipps Universität Marburg = Philipps University of Marburg 2011-01-29 https://hal.science/hal-00664099 https://hal.science/hal-00664099/document https://hal.science/hal-00664099/file/10344_2011_499_ReferencePDF.pdf https://doi.org/10.1007/s10344-011-0499-9 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10344-011-0499-9 hal-00664099 https://hal.science/hal-00664099 https://hal.science/hal-00664099/document https://hal.science/hal-00664099/file/10344_2011_499_ReferencePDF.pdf doi:10.1007/s10344-011-0499-9 info:eu-repo/semantics/OpenAccess ISSN: 1612-4642 European Journal of Wildlife Research https://hal.science/hal-00664099 European Journal of Wildlife Research, 2011, 57 (4), pp.873-885. ⟨10.1007/s10344-011-0499-9⟩ Barrier Epidermis Wild mammals Biotope adaptation Glycolipids info:eu-repo/semantics/article Journal articles 2011 ftccsdartic https://doi.org/10.1007/s10344-011-0499-9 2024-01-13T23:30:33Z International audience Based on the combination of standard light and transmission electron microscopy, cryo-SEM, immunohistochemistry and a new sensitive glycolipid histochemical technique (5-hexadecanoylaminofluorescein staining, laser scanning microscopy), including densitometrical evaluation, our approach gives for the first time an overview of the specific biology of the epidermal permeability barrier in wild mammals (20 species from five orders), living under varying (aquatic or moist to dry) habitat conditions. The results obtained emphasised that the barrier region in most of the species studied is a continuous zone (thickness, 0.1 and 3 μm) between the upper cells of the stratum granulosum and the inner cells of the stratum corneum conjunctum, normally present as a homogeneous glycolipid layer originating from fusion of lamellar body contents after exocytotic activities of the granular cells. However, this finding did not apply to all of the species studied, i.e., the Wild boar, the Common seal and the three large species with a very thick vital epidermis, the African elephant, the hippopotamus and the common dolphin, exhibited variations from the basic scheme. Densitometric evaluation of the 5-hexadecanoylaminofluorescein staining revealed that reaction intensity was not only generally related to the habitat conditions but also to vital epidermis thickness and hair density. The immunohistochemical demonstration of Na/H exchanger 1 corroborated for all wild mammals studied that this important regulator of pH conditions during barrier formation is continuously produced in the epidermis. The variations in barrier biology observed for some species obviously had to be developed in relation to animal size (or body size area) and hair coat density, but, particularly, by the specific adaptation of certain mammalian groups to the aquatic environment. In the latter case, the typical barrier zone system was lost, as in the hippopotamus or the cetaceans. Article in Journal/Newspaper common seal Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) European Journal of Wildlife Research 57 4 873 885 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Barrier Epidermis Wild mammals Biotope adaptation Glycolipids |
spellingShingle |
Barrier Epidermis Wild mammals Biotope adaptation Glycolipids Meyer, Wilfried Schmidt, Judith Kacza, Johannes Busche, Roger Naim, Hassan Y. Jacob, Ralf Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates |
topic_facet |
Barrier Epidermis Wild mammals Biotope adaptation Glycolipids |
description |
International audience Based on the combination of standard light and transmission electron microscopy, cryo-SEM, immunohistochemistry and a new sensitive glycolipid histochemical technique (5-hexadecanoylaminofluorescein staining, laser scanning microscopy), including densitometrical evaluation, our approach gives for the first time an overview of the specific biology of the epidermal permeability barrier in wild mammals (20 species from five orders), living under varying (aquatic or moist to dry) habitat conditions. The results obtained emphasised that the barrier region in most of the species studied is a continuous zone (thickness, 0.1 and 3 μm) between the upper cells of the stratum granulosum and the inner cells of the stratum corneum conjunctum, normally present as a homogeneous glycolipid layer originating from fusion of lamellar body contents after exocytotic activities of the granular cells. However, this finding did not apply to all of the species studied, i.e., the Wild boar, the Common seal and the three large species with a very thick vital epidermis, the African elephant, the hippopotamus and the common dolphin, exhibited variations from the basic scheme. Densitometric evaluation of the 5-hexadecanoylaminofluorescein staining revealed that reaction intensity was not only generally related to the habitat conditions but also to vital epidermis thickness and hair density. The immunohistochemical demonstration of Na/H exchanger 1 corroborated for all wild mammals studied that this important regulator of pH conditions during barrier formation is continuously produced in the epidermis. The variations in barrier biology observed for some species obviously had to be developed in relation to animal size (or body size area) and hair coat density, but, particularly, by the specific adaptation of certain mammalian groups to the aquatic environment. In the latter case, the typical barrier zone system was lost, as in the hippopotamus or the cetaceans. |
author2 |
Institute for Anatomy University of Veterinary Medicine Hannover, Foundation Institute of Veterinary Anatomy Universität Leipzig Institute for Physiological Chemistry Institute of Cytobiology and Cytopathology Philipps Universität Marburg = Philipps University of Marburg |
format |
Article in Journal/Newspaper |
author |
Meyer, Wilfried Schmidt, Judith Kacza, Johannes Busche, Roger Naim, Hassan Y. Jacob, Ralf |
author_facet |
Meyer, Wilfried Schmidt, Judith Kacza, Johannes Busche, Roger Naim, Hassan Y. Jacob, Ralf |
author_sort |
Meyer, Wilfried |
title |
Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates |
title_short |
Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates |
title_full |
Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates |
title_fullStr |
Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates |
title_full_unstemmed |
Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates |
title_sort |
basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.science/hal-00664099 https://hal.science/hal-00664099/document https://hal.science/hal-00664099/file/10344_2011_499_ReferencePDF.pdf https://doi.org/10.1007/s10344-011-0499-9 |
genre |
common seal |
genre_facet |
common seal |
op_source |
ISSN: 1612-4642 European Journal of Wildlife Research https://hal.science/hal-00664099 European Journal of Wildlife Research, 2011, 57 (4), pp.873-885. ⟨10.1007/s10344-011-0499-9⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10344-011-0499-9 hal-00664099 https://hal.science/hal-00664099 https://hal.science/hal-00664099/document https://hal.science/hal-00664099/file/10344_2011_499_ReferencePDF.pdf doi:10.1007/s10344-011-0499-9 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s10344-011-0499-9 |
container_title |
European Journal of Wildlife Research |
container_volume |
57 |
container_issue |
4 |
container_start_page |
873 |
op_container_end_page |
885 |
_version_ |
1790599050981015552 |