Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers
International audience Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syrin...
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2008
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ftccsdartic:oai:HAL:hal-00645718v1 2023-05-15T15:57:37+02:00 Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers Gagnaire, Béatrice Duchemin, Matthieu Auffret, Michel Thomas-Guyon, Hélène Renault, Tristan Laboratoire de Génétique et Pathologie (LGP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Centre de Recherche sur les Ecosystèmes Littoraux Anthropisés (CRELA) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2008 https://hal.univ-brest.fr/hal-00645718 https://hal.univ-brest.fr/hal-00645718/document https://hal.univ-brest.fr/hal-00645718/file/gagnaire_duchemin_ALR_2008.pdf https://doi.org/10.1051/alr:2008009 en eng HAL CCSD EDP Sciences info:eu-repo/semantics/altIdentifier/doi/10.1051/alr:2008009 hal-00645718 https://hal.univ-brest.fr/hal-00645718 https://hal.univ-brest.fr/hal-00645718/document https://hal.univ-brest.fr/hal-00645718/file/gagnaire_duchemin_ALR_2008.pdf doi:10.1051/alr:2008009 info:eu-repo/semantics/OpenAccess ISSN: 0990-7440 EISSN: 1765-2952 Aquatic Living Resources https://hal.univ-brest.fr/hal-00645718 Aquatic Living Resources, EDP Sciences, 2008, 21, pp.39-43. ⟨10.1051/alr:2008009⟩ Flow cytometry Hemocytes Phagocytosis Crassostrea gigas [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2008 ftccsdartic https://doi.org/10.1051/alr:2008009 2021-12-19T03:32:26Z International audience Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers (EPICS XL4®, Beckman Coulter and FacsCalibur®, Becton Dickinson) and two sites of hemocyte collection (pericardial cavity and adductor muscle sinus) were compared for the analysis of hemocyte parameters in the Pacific oyster, Crassostrea gigas. Hemolymph cells were analyzed in terms of their number and organelle contents. Cell mortality, phagocytosis, non specific esterase, extension of the lysosomal compartment and production of reactive oxygen species were quantified. The results showed that the phagocytic index was higher for hemocytes obtained in the muscle sinus hemolymph. The results ar Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Coulter ENVELOPE(-58.033,-58.033,-83.283,-83.283) |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Flow cytometry Hemocytes Phagocytosis Crassostrea gigas [SDE]Environmental Sciences |
spellingShingle |
Flow cytometry Hemocytes Phagocytosis Crassostrea gigas [SDE]Environmental Sciences Gagnaire, Béatrice Duchemin, Matthieu Auffret, Michel Thomas-Guyon, Hélène Renault, Tristan Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
topic_facet |
Flow cytometry Hemocytes Phagocytosis Crassostrea gigas [SDE]Environmental Sciences |
description |
International audience Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers (EPICS XL4®, Beckman Coulter and FacsCalibur®, Becton Dickinson) and two sites of hemocyte collection (pericardial cavity and adductor muscle sinus) were compared for the analysis of hemocyte parameters in the Pacific oyster, Crassostrea gigas. Hemolymph cells were analyzed in terms of their number and organelle contents. Cell mortality, phagocytosis, non specific esterase, extension of the lysosomal compartment and production of reactive oxygen species were quantified. The results showed that the phagocytic index was higher for hemocytes obtained in the muscle sinus hemolymph. The results ar |
author2 |
Laboratoire de Génétique et Pathologie (LGP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Centre de Recherche sur les Ecosystèmes Littoraux Anthropisés (CRELA) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Gagnaire, Béatrice Duchemin, Matthieu Auffret, Michel Thomas-Guyon, Hélène Renault, Tristan |
author_facet |
Gagnaire, Béatrice Duchemin, Matthieu Auffret, Michel Thomas-Guyon, Hélène Renault, Tristan |
author_sort |
Gagnaire, Béatrice |
title |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_short |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_full |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_fullStr |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_full_unstemmed |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_sort |
comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the pacific oyster, crassostrea gigas using two types of flow cytometers |
publisher |
HAL CCSD |
publishDate |
2008 |
url |
https://hal.univ-brest.fr/hal-00645718 https://hal.univ-brest.fr/hal-00645718/document https://hal.univ-brest.fr/hal-00645718/file/gagnaire_duchemin_ALR_2008.pdf https://doi.org/10.1051/alr:2008009 |
long_lat |
ENVELOPE(-58.033,-58.033,-83.283,-83.283) |
geographic |
Pacific Coulter |
geographic_facet |
Pacific Coulter |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0990-7440 EISSN: 1765-2952 Aquatic Living Resources https://hal.univ-brest.fr/hal-00645718 Aquatic Living Resources, EDP Sciences, 2008, 21, pp.39-43. ⟨10.1051/alr:2008009⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1051/alr:2008009 hal-00645718 https://hal.univ-brest.fr/hal-00645718 https://hal.univ-brest.fr/hal-00645718/document https://hal.univ-brest.fr/hal-00645718/file/gagnaire_duchemin_ALR_2008.pdf doi:10.1051/alr:2008009 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1051/alr:2008009 |
_version_ |
1766393283983966208 |