Novel proteins from the calcifying shell matrix of the Pacific oyster Crassostrea gigas.

10 pages International audience The shell of the Pacific oyster Crassostrea gigas is composed of more than 99% CaCO(3) and of around 0.5% of occluded organic matrix. According to classical views, this matrix is supposed to regulate the shell mineral deposition. In this study, we developed one of the...

Full description

Bibliographic Details
Published in:Marine Biotechnology
Main Authors: Marie, Benjamin, Zanella-Cléon, Isabelle, Guichard, Nathalie, Becchi, Michel, Marin, Frédéric
Other Authors: Biogéosciences UMR 6282 Dijon (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines Lyon (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Financial support provided by INSU (Action INTERVIE 2010) and by COST action TD0903 (Davorin Medakovic 2009-2013), and by the "Conseil Régional de Bourgogne" (Dijon, France).
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2011
Subjects:
Biomineralization
Calcium carbonate
EGF-like domain
Foliated calcite
Mollusk shell
Organic matrix
Proteomics
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Pacific
Crassostrea gigas
Pacific oyster
Online Access:https://hal.archives-ouvertes.fr/hal-00638291
https://doi.org/10.1007/s10126-011-9379-2
Description
Summary:10 pages International audience The shell of the Pacific oyster Crassostrea gigas is composed of more than 99% CaCO(3) and of around 0.5% of occluded organic matrix. According to classical views, this matrix is supposed to regulate the shell mineral deposition. In this study, we developed one of the first proteomic approaches applied to mollusk shell in order to characterise the calcifying matrix proteins. The insoluble organic matrix, purified after demineralisation of the shell powder, was digested with trypsin enzyme, and separated on nano-LC, prior to nanospray quadrupole/time-of-flight analysis. MS/MS spectra were searched against the above 220,000 EST sequences available in the public database for Crassostrea. Using this approach, we were able to identify partial or full-length sequence transcripts that encode eight novel shell matrix proteins.

Similar Items