In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg)
International audience The effects of a soluble fraction of light cycle oil (LCO) on haemocyte parameters, phenoloxidase (PO) activity and mRNA expression of immune-related genes, in the Pacific oyster, Crassostrea gigas, were tested after seven days of exposure and two weeks of recovery period. Fiv...
Published in: | Aquatic Toxicology |
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Language: | English |
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Online Access: | https://hal.univ-brest.fr/hal-00638192 https://doi.org/10.1016/j.aquatox.2009.08.005 |
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ftccsdartic:oai:HAL:hal-00638192v1 2023-05-15T15:57:35+02:00 In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg) Bado-Nilles, Anne Renault, Tristan Faury, Nicole Le Floch, Stéphane Quentel, Claire Auffret, Michel Thomas-Guyon, Hélène LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre) Cedre Laboratoire de Génétique et Pathologie (LGP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Site de Ploufragan-Plouzané (AFSSA) Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2010 https://hal.univ-brest.fr/hal-00638192 https://doi.org/10.1016/j.aquatox.2009.08.005 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2009.08.005 hal-00638192 https://hal.univ-brest.fr/hal-00638192 doi:10.1016/j.aquatox.2009.08.005 ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00638192 Aquatic Toxicology, Elsevier, 2010, 97 (3), pp.196-203. ⟨10.1016/j.aquatox.2009.08.005⟩ Crassostrea gigas Light cycle oil Immune parameters Bioaccumulation Gene transcription [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2010 ftccsdartic https://doi.org/10.1016/j.aquatox.2009.08.005 2021-12-19T03:33:14Z International audience The effects of a soluble fraction of light cycle oil (LCO) on haemocyte parameters, phenoloxidase (PO) activity and mRNA expression of immune-related genes, in the Pacific oyster, Crassostrea gigas, were tested after seven days of exposure and two weeks of recovery period. Five polycyclic aromatic hydrocarbons (PAHs) out of ten detected in tank water had bioaccumulated at the end of the contamination period. The concentration of PAHs in oyster tissues decreased during the recovery period and 14 days after the exposure, 69% of bioaccumulated PAHs were detected in contaminated oysters. The exposure induced severe oyster mortality (21%), external and internal green colouration of the shell and a significant decrease of PO activity. The mRNA expression of several genes was altered. As a conclusion, a modulation of immune-related parameters was demonstrated using three different approaches, namely cellular (flow cytometry), biochemical (spectrophotometry) and genomics (gene transcription) in oysters after contact with soluble fraction of LCO. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Aquatic Toxicology 97 3 196 203 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Crassostrea gigas Light cycle oil Immune parameters Bioaccumulation Gene transcription [SDE]Environmental Sciences |
spellingShingle |
Crassostrea gigas Light cycle oil Immune parameters Bioaccumulation Gene transcription [SDE]Environmental Sciences Bado-Nilles, Anne Renault, Tristan Faury, Nicole Le Floch, Stéphane Quentel, Claire Auffret, Michel Thomas-Guyon, Hélène In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg) |
topic_facet |
Crassostrea gigas Light cycle oil Immune parameters Bioaccumulation Gene transcription [SDE]Environmental Sciences |
description |
International audience The effects of a soluble fraction of light cycle oil (LCO) on haemocyte parameters, phenoloxidase (PO) activity and mRNA expression of immune-related genes, in the Pacific oyster, Crassostrea gigas, were tested after seven days of exposure and two weeks of recovery period. Five polycyclic aromatic hydrocarbons (PAHs) out of ten detected in tank water had bioaccumulated at the end of the contamination period. The concentration of PAHs in oyster tissues decreased during the recovery period and 14 days after the exposure, 69% of bioaccumulated PAHs were detected in contaminated oysters. The exposure induced severe oyster mortality (21%), external and internal green colouration of the shell and a significant decrease of PO activity. The mRNA expression of several genes was altered. As a conclusion, a modulation of immune-related parameters was demonstrated using three different approaches, namely cellular (flow cytometry), biochemical (spectrophotometry) and genomics (gene transcription) in oysters after contact with soluble fraction of LCO. |
author2 |
LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre) Cedre Laboratoire de Génétique et Pathologie (LGP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Site de Ploufragan-Plouzané (AFSSA) Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Bado-Nilles, Anne Renault, Tristan Faury, Nicole Le Floch, Stéphane Quentel, Claire Auffret, Michel Thomas-Guyon, Hélène |
author_facet |
Bado-Nilles, Anne Renault, Tristan Faury, Nicole Le Floch, Stéphane Quentel, Claire Auffret, Michel Thomas-Guyon, Hélène |
author_sort |
Bado-Nilles, Anne |
title |
In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg) |
title_short |
In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg) |
title_full |
In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg) |
title_fullStr |
In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg) |
title_full_unstemmed |
In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg) |
title_sort |
in vivo effects of lco soluble fraction on immune-related functions and gene transcription in the pacific oyster, crassostrea gigas (thunberg) |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.univ-brest.fr/hal-00638192 https://doi.org/10.1016/j.aquatox.2009.08.005 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00638192 Aquatic Toxicology, Elsevier, 2010, 97 (3), pp.196-203. ⟨10.1016/j.aquatox.2009.08.005⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2009.08.005 hal-00638192 https://hal.univ-brest.fr/hal-00638192 doi:10.1016/j.aquatox.2009.08.005 |
op_doi |
https://doi.org/10.1016/j.aquatox.2009.08.005 |
container_title |
Aquatic Toxicology |
container_volume |
97 |
container_issue |
3 |
container_start_page |
196 |
op_container_end_page |
203 |
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1766393248614449152 |