Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet
International audience During the austral spring and summer months, the Antarctic limpet colonizes intertidal environments in the Western Antarctica Peninsula region. The species is divided into a permanently sub-littoral and a seasonally intertidal, migratory subpopulation. We investigate the physi...
Published in: | Marine Environmental Research |
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Language: | English |
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2010
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Online Access: | https://hal.archives-ouvertes.fr/hal-00565103 https://hal.archives-ouvertes.fr/hal-00565103/document https://hal.archives-ouvertes.fr/hal-00565103/file/PEER_stage2_10.1016%252Fj.marenvres.2009.09.003.pdf https://doi.org/10.1016/j.marenvres.2009.09.003 |
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ftccsdartic:oai:HAL:hal-00565103v1 2023-05-15T13:50:10+02:00 Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet Weihe, Ellen Kriews, Michael Abele, Doris 2010-02-11 https://hal.archives-ouvertes.fr/hal-00565103 https://hal.archives-ouvertes.fr/hal-00565103/document https://hal.archives-ouvertes.fr/hal-00565103/file/PEER_stage2_10.1016%252Fj.marenvres.2009.09.003.pdf https://doi.org/10.1016/j.marenvres.2009.09.003 en eng HAL CCSD Elsevier science info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marenvres.2009.09.003 hal-00565103 https://hal.archives-ouvertes.fr/hal-00565103 https://hal.archives-ouvertes.fr/hal-00565103/document https://hal.archives-ouvertes.fr/hal-00565103/file/PEER_stage2_10.1016%252Fj.marenvres.2009.09.003.pdf doi:10.1016/j.marenvres.2009.09.003 info:eu-repo/semantics/OpenAccess ISSN: 0141-1136 EISSN: 1879-0291 Marine Environmental Research https://hal.archives-ouvertes.fr/hal-00565103 Marine Environmental Research, Elsevier science, 2010, 69 (3), pp.127. ⟨10.1016/j.marenvres.2009.09.003⟩ Nacella concinna oxidative stress glutathione heavy metals hypoxia air exposure info:eu-repo/semantics/article Journal articles 2010 ftccsdartic https://doi.org/10.1016/j.marenvres.2009.09.003 2021-12-19T03:43:12Z International audience During the austral spring and summer months, the Antarctic limpet colonizes intertidal environments in the Western Antarctica Peninsula region. The species is divided into a permanently sub-littoral and a seasonally intertidal, migratory subpopulation. We investigate the physiological differentiation between the two limpet groups to identify cellular and molecular changes that accompany adaptation of stenothermal Antarctic invertebrates to life under more stressful intertidal habitat conditions. A major difference between the two groups is the significantly higher concentrations of heavy metals (Fe, Al, Zn) from ingested sediments in sub-littoral limpet digestive glands (DG), associated with higher rates of reactive oxygen species (ROS) formation in this organ. ROS formation is accompanied by significantly higher SOD activity in sub-littoral limpet DG. These high SOD activities are, however, not conserved during either air exposure or hypoxic stress exposure of the sub-littoral limpets, when ROS production is slowed due to the absence of oxygen. The intertidal animals maintain higher levels of SOD and also conserve catalase activity at higher levels during hypoxia or air exposure compared to sub-littoral individuals under the same exposure conditions. More oxidized redox potential in gills and foot muscle and higher antioxidant enzyme activities in gills indicate that intertidal limpets maintain more oxygenated tissues during air exposure, in keeping with more intense ventilation of shell water in the intertidal subpopulation which migrates up the shore in the spring and down in the autumn. An increase of the redox ratio (GSSG/GSH) and accumulation of the lipid oxidation derived malonedialdehyde in intertidal limpet foot muscle during 12h of exposure to air shows that indeed this tissue becomes more oxidized before the limpets eventually contract their shells tightly to minimize water loss and eventually become anaerobic. Intertidal limpets obviously avoid early onset of anaerobic energy ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Antarctic The Antarctic Austral Nacella ENVELOPE(-60.783,-60.783,-62.467,-62.467) Marine Environmental Research 69 3 127 135 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Nacella concinna oxidative stress glutathione heavy metals hypoxia air exposure |
spellingShingle |
Nacella concinna oxidative stress glutathione heavy metals hypoxia air exposure Weihe, Ellen Kriews, Michael Abele, Doris Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet |
topic_facet |
Nacella concinna oxidative stress glutathione heavy metals hypoxia air exposure |
description |
International audience During the austral spring and summer months, the Antarctic limpet colonizes intertidal environments in the Western Antarctica Peninsula region. The species is divided into a permanently sub-littoral and a seasonally intertidal, migratory subpopulation. We investigate the physiological differentiation between the two limpet groups to identify cellular and molecular changes that accompany adaptation of stenothermal Antarctic invertebrates to life under more stressful intertidal habitat conditions. A major difference between the two groups is the significantly higher concentrations of heavy metals (Fe, Al, Zn) from ingested sediments in sub-littoral limpet digestive glands (DG), associated with higher rates of reactive oxygen species (ROS) formation in this organ. ROS formation is accompanied by significantly higher SOD activity in sub-littoral limpet DG. These high SOD activities are, however, not conserved during either air exposure or hypoxic stress exposure of the sub-littoral limpets, when ROS production is slowed due to the absence of oxygen. The intertidal animals maintain higher levels of SOD and also conserve catalase activity at higher levels during hypoxia or air exposure compared to sub-littoral individuals under the same exposure conditions. More oxidized redox potential in gills and foot muscle and higher antioxidant enzyme activities in gills indicate that intertidal limpets maintain more oxygenated tissues during air exposure, in keeping with more intense ventilation of shell water in the intertidal subpopulation which migrates up the shore in the spring and down in the autumn. An increase of the redox ratio (GSSG/GSH) and accumulation of the lipid oxidation derived malonedialdehyde in intertidal limpet foot muscle during 12h of exposure to air shows that indeed this tissue becomes more oxidized before the limpets eventually contract their shells tightly to minimize water loss and eventually become anaerobic. Intertidal limpets obviously avoid early onset of anaerobic energy ... |
format |
Article in Journal/Newspaper |
author |
Weihe, Ellen Kriews, Michael Abele, Doris |
author_facet |
Weihe, Ellen Kriews, Michael Abele, Doris |
author_sort |
Weihe, Ellen |
title |
Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet |
title_short |
Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet |
title_full |
Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet |
title_fullStr |
Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet |
title_full_unstemmed |
Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet |
title_sort |
differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the antarctic limpet |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.archives-ouvertes.fr/hal-00565103 https://hal.archives-ouvertes.fr/hal-00565103/document https://hal.archives-ouvertes.fr/hal-00565103/file/PEER_stage2_10.1016%252Fj.marenvres.2009.09.003.pdf https://doi.org/10.1016/j.marenvres.2009.09.003 |
long_lat |
ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
geographic |
Antarctic The Antarctic Austral Nacella |
geographic_facet |
Antarctic The Antarctic Austral Nacella |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
ISSN: 0141-1136 EISSN: 1879-0291 Marine Environmental Research https://hal.archives-ouvertes.fr/hal-00565103 Marine Environmental Research, Elsevier science, 2010, 69 (3), pp.127. ⟨10.1016/j.marenvres.2009.09.003⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marenvres.2009.09.003 hal-00565103 https://hal.archives-ouvertes.fr/hal-00565103 https://hal.archives-ouvertes.fr/hal-00565103/document https://hal.archives-ouvertes.fr/hal-00565103/file/PEER_stage2_10.1016%252Fj.marenvres.2009.09.003.pdf doi:10.1016/j.marenvres.2009.09.003 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.marenvres.2009.09.003 |
container_title |
Marine Environmental Research |
container_volume |
69 |
container_issue |
3 |
container_start_page |
127 |
op_container_end_page |
135 |
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1766253167795765248 |