First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe).
International audience Ciguatera fish poisoning (CFP) is a human foodborne intoxication caused by ingestion of tropical fishes contaminated with the potent polyether toxins known as ciguatoxins (CTXs). These toxins are issued from Gambierdiscus species of dinoflagellates. Herbivorous fish accumulate...
Published in: | Analytical Chemistry |
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Online Access: | https://hal.archives-ouvertes.fr/hal-00506113 https://doi.org/10.1021/ac100516q |
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ftccsdartic:oai:HAL:hal-00506113v1 2023-05-15T17:36:00+02:00 First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe). Otero, Paz Pérez, Sheila Alfonso, Amparo Vale, Carmen Rodríguez, Paula Gouveia, Neide N Gouveia, Nuno Delgado, João Vale, Paulo Hirama, Masahiro Ishihara, Yuuki Molgó, Jordi Botana, Luis M Departamento de Farmacologia Facultad de Veterianaria Universidad de Santiago de Compostela Spain (USC) Direco Regional das Pescas Instituto Nacional dos Recursos Biolgicos (IPIMAR (INRB-IPIMAR)) Instituto Nacional dos Recursos Biolgicos Graduate School of Science, Department of Chemistry Tohoku University Sendai Laboratoire de neurobiologie cellulaire et moléculaire (NBCM) Centre National de la Recherche Scientifique (CNRS) Institut de Neurobiologie Alfred Fessard (INAF) 2010-06-17 https://hal.archives-ouvertes.fr/hal-00506113 https://doi.org/10.1021/ac100516q en eng HAL CCSD American Chemical Society info:eu-repo/semantics/altIdentifier/doi/10.1021/ac100516q info:eu-repo/semantics/altIdentifier/pmid/20557036 hal-00506113 https://hal.archives-ouvertes.fr/hal-00506113 doi:10.1021/ac100516q PUBMED: 20557036 ISSN: 0003-2700 EISSN: 1520-6882 Analytical Chemistry https://hal.archives-ouvertes.fr/hal-00506113 Analytical Chemistry, American Chemical Society, 2010, 82 (14), pp.6032-9. ⟨10.1021/ac100516q⟩ [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] info:eu-repo/semantics/article Journal articles 2010 ftccsdartic https://doi.org/10.1021/ac100516q 2020-12-26T07:41:01Z International audience Ciguatera fish poisoning (CFP) is a human foodborne intoxication caused by ingestion of tropical fishes contaminated with the potent polyether toxins known as ciguatoxins (CTXs). These toxins are issued from Gambierdiscus species of dinoflagellates. Herbivorous fish accumulate these toxins in their musculature and viscera after ingesting dinoflagellates. Epidemiological studies showed that CFP has been present in areas between 35 degrees North and 35 degrees South latitude, mainly, Indo-pacific and Caribbean areas, but not in waters closed to European and African continent. In the present paper, a specimen of Seriola dumerili weighing 70 kg and a smaller Seriola fasciata specimen, captured in waters belonging to Selvagens Islands (Madeira Arquipelago), were analyzed. Fishes from this genus were implicated in previous suspected ciguatera poisoning outbreaks in the Portuguese Madeira Arquipelago in the North Atlantic Ocean. Analysis was performed by two approaches, a functional method using cerebellar granule cells and by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) method. The study was carried out in one portion of the tail muscle of Seriola fasciata and five parts of the body of Seriola dumerili (tail muscle, head, ventral muscle, mid muscle, and liver). The functional method consisted in the modification of the inward sodium current in cerebellar granule cells and the chemical method was a high resolution chromatography, which allowed elucidating the toxin profile in the samples. In addition, UPLC-MS technique was optimized and used for detecting and quantifying CTXs for the first time. After fish extraction and clean up, the chromatograms revealed the presence of CTX-1B at 1111.6 m/z, CTX-3C at 1023.5 m/z, a CTX analogue at 1040.6 m/z, and a CTX from the Caribbean or Indic waters at 1141.6 m/z. Therefore, the results obtained in the present paper for both methods confirm, for the first time, the presence of CTX in fish from Madeira Arquipelago. Article in Journal/Newspaper North Atlantic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Analytical Chemistry 82 14 6032 6039 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] |
spellingShingle |
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Otero, Paz Pérez, Sheila Alfonso, Amparo Vale, Carmen Rodríguez, Paula Gouveia, Neide N Gouveia, Nuno Delgado, João Vale, Paulo Hirama, Masahiro Ishihara, Yuuki Molgó, Jordi Botana, Luis M First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe). |
topic_facet |
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] |
description |
International audience Ciguatera fish poisoning (CFP) is a human foodborne intoxication caused by ingestion of tropical fishes contaminated with the potent polyether toxins known as ciguatoxins (CTXs). These toxins are issued from Gambierdiscus species of dinoflagellates. Herbivorous fish accumulate these toxins in their musculature and viscera after ingesting dinoflagellates. Epidemiological studies showed that CFP has been present in areas between 35 degrees North and 35 degrees South latitude, mainly, Indo-pacific and Caribbean areas, but not in waters closed to European and African continent. In the present paper, a specimen of Seriola dumerili weighing 70 kg and a smaller Seriola fasciata specimen, captured in waters belonging to Selvagens Islands (Madeira Arquipelago), were analyzed. Fishes from this genus were implicated in previous suspected ciguatera poisoning outbreaks in the Portuguese Madeira Arquipelago in the North Atlantic Ocean. Analysis was performed by two approaches, a functional method using cerebellar granule cells and by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) method. The study was carried out in one portion of the tail muscle of Seriola fasciata and five parts of the body of Seriola dumerili (tail muscle, head, ventral muscle, mid muscle, and liver). The functional method consisted in the modification of the inward sodium current in cerebellar granule cells and the chemical method was a high resolution chromatography, which allowed elucidating the toxin profile in the samples. In addition, UPLC-MS technique was optimized and used for detecting and quantifying CTXs for the first time. After fish extraction and clean up, the chromatograms revealed the presence of CTX-1B at 1111.6 m/z, CTX-3C at 1023.5 m/z, a CTX analogue at 1040.6 m/z, and a CTX from the Caribbean or Indic waters at 1141.6 m/z. Therefore, the results obtained in the present paper for both methods confirm, for the first time, the presence of CTX in fish from Madeira Arquipelago. |
author2 |
Departamento de Farmacologia Facultad de Veterianaria Universidad de Santiago de Compostela Spain (USC) Direco Regional das Pescas Instituto Nacional dos Recursos Biolgicos (IPIMAR (INRB-IPIMAR)) Instituto Nacional dos Recursos Biolgicos Graduate School of Science, Department of Chemistry Tohoku University Sendai Laboratoire de neurobiologie cellulaire et moléculaire (NBCM) Centre National de la Recherche Scientifique (CNRS) Institut de Neurobiologie Alfred Fessard (INAF) |
format |
Article in Journal/Newspaper |
author |
Otero, Paz Pérez, Sheila Alfonso, Amparo Vale, Carmen Rodríguez, Paula Gouveia, Neide N Gouveia, Nuno Delgado, João Vale, Paulo Hirama, Masahiro Ishihara, Yuuki Molgó, Jordi Botana, Luis M |
author_facet |
Otero, Paz Pérez, Sheila Alfonso, Amparo Vale, Carmen Rodríguez, Paula Gouveia, Neide N Gouveia, Nuno Delgado, João Vale, Paulo Hirama, Masahiro Ishihara, Yuuki Molgó, Jordi Botana, Luis M |
author_sort |
Otero, Paz |
title |
First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe). |
title_short |
First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe). |
title_full |
First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe). |
title_fullStr |
First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe). |
title_full_unstemmed |
First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe). |
title_sort |
first toxin profile of ciguateric fish in madeira arquipelago (europe). |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.archives-ouvertes.fr/hal-00506113 https://doi.org/10.1021/ac100516q |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 0003-2700 EISSN: 1520-6882 Analytical Chemistry https://hal.archives-ouvertes.fr/hal-00506113 Analytical Chemistry, American Chemical Society, 2010, 82 (14), pp.6032-9. ⟨10.1021/ac100516q⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1021/ac100516q info:eu-repo/semantics/altIdentifier/pmid/20557036 hal-00506113 https://hal.archives-ouvertes.fr/hal-00506113 doi:10.1021/ac100516q PUBMED: 20557036 |
op_doi |
https://doi.org/10.1021/ac100516q |
container_title |
Analytical Chemistry |
container_volume |
82 |
container_issue |
14 |
container_start_page |
6032 |
op_container_end_page |
6039 |
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