Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas.
International audience To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different...
Published in: | Animal Genetics |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2008
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Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-00432623 https://doi.org/10.1111/j.1365-2052.2008.01776.x |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
α-amylase bivalve gene expression ingestion MESH: Amylases MESH: Animals MESH: Base Sequence MESH: DNA Primers MESH: Feeding Behavior MESH: Kinetics MESH: Ostreidae MESH: Polymerase Chain Reaction MESH: Polymorphism Genetic Restriction Fragment Length [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology |
spellingShingle |
α-amylase bivalve gene expression ingestion MESH: Amylases MESH: Animals MESH: Base Sequence MESH: DNA Primers MESH: Feeding Behavior MESH: Kinetics MESH: Ostreidae MESH: Polymerase Chain Reaction MESH: Polymorphism Genetic Restriction Fragment Length [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Huvet, Arnaud Jeffroy, F. Fabioux, Caroline Daniel, J. Y. Quillien, V. Van Wormhoudt, A. Moal, J. Samain, J. F. Boudry, Pierre Pouvreau, Stéphane Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas. |
topic_facet |
α-amylase bivalve gene expression ingestion MESH: Amylases MESH: Animals MESH: Base Sequence MESH: DNA Primers MESH: Feeding Behavior MESH: Kinetics MESH: Ostreidae MESH: Polymerase Chain Reaction MESH: Polymorphism Genetic Restriction Fragment Length [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology |
description |
International audience To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to ingestion than to absorption efficiency. Additionally, relative mRNA levels of the two amylase cDNAs were also strongly associated with amylase gene polymorphism, possibly reflecting variation in an undefined regulatory region, although no corresponding variation was observed in specific amylase activity. Amylase gene sequences were determined for each genotype, showing the existence of only synonymous or functionally equivalent non-synonymous polymorphisms. The observed associations among growth, food consumption-related traits and amylase gene polymorphism are therefore more likely to be related to variation in the level of amylase gene expression than to functional enzymatic variants. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Biologie des organismes marins et écosystèmes (BOME) Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN) Station de Biologie Marine de Concarneau Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE) Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN) |
format |
Article in Journal/Newspaper |
author |
Huvet, Arnaud Jeffroy, F. Fabioux, Caroline Daniel, J. Y. Quillien, V. Van Wormhoudt, A. Moal, J. Samain, J. F. Boudry, Pierre Pouvreau, Stéphane |
author_facet |
Huvet, Arnaud Jeffroy, F. Fabioux, Caroline Daniel, J. Y. Quillien, V. Van Wormhoudt, A. Moal, J. Samain, J. F. Boudry, Pierre Pouvreau, Stéphane |
author_sort |
Huvet, Arnaud |
title |
Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas. |
title_short |
Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas. |
title_full |
Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas. |
title_fullStr |
Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas. |
title_full_unstemmed |
Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas. |
title_sort |
association among growth, food consumption-related traits and amylase gene polymorphism in the pacific oyster crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2008 |
url |
https://hal.archives-ouvertes.fr/hal-00432623 https://doi.org/10.1111/j.1365-2052.2008.01776.x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0268-9146 EISSN: 1365-2052 Animal Genetics https://hal.archives-ouvertes.fr/hal-00432623 Animal Genetics, Wiley-Blackwell, 2008, 39 (6), pp.662-5. ⟨10.1111/j.1365-2052.2008.01776.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2052.2008.01776.x info:eu-repo/semantics/altIdentifier/pmid/18717666 hal-00432623 https://hal.archives-ouvertes.fr/hal-00432623 doi:10.1111/j.1365-2052.2008.01776.x PUBMED: 18717666 |
op_doi |
https://doi.org/10.1111/j.1365-2052.2008.01776.x |
container_title |
Animal Genetics |
container_volume |
39 |
container_issue |
6 |
container_start_page |
662 |
op_container_end_page |
665 |
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1766393914299777024 |
spelling |
ftccsdartic:oai:HAL:hal-00432623v1 2023-05-15T15:58:11+02:00 Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas. Huvet, Arnaud Jeffroy, F. Fabioux, Caroline Daniel, J. Y. Quillien, V. Van Wormhoudt, A. Moal, J. Samain, J. F. Boudry, Pierre Pouvreau, Stéphane Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Biologie des organismes marins et écosystèmes (BOME) Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN) Station de Biologie Marine de Concarneau Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE) Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN) 2008-12 https://hal.archives-ouvertes.fr/hal-00432623 https://doi.org/10.1111/j.1365-2052.2008.01776.x en eng HAL CCSD Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2052.2008.01776.x info:eu-repo/semantics/altIdentifier/pmid/18717666 hal-00432623 https://hal.archives-ouvertes.fr/hal-00432623 doi:10.1111/j.1365-2052.2008.01776.x PUBMED: 18717666 ISSN: 0268-9146 EISSN: 1365-2052 Animal Genetics https://hal.archives-ouvertes.fr/hal-00432623 Animal Genetics, Wiley-Blackwell, 2008, 39 (6), pp.662-5. ⟨10.1111/j.1365-2052.2008.01776.x⟩ α-amylase bivalve gene expression ingestion MESH: Amylases MESH: Animals MESH: Base Sequence MESH: DNA Primers MESH: Feeding Behavior MESH: Kinetics MESH: Ostreidae MESH: Polymerase Chain Reaction MESH: Polymorphism Genetic Restriction Fragment Length [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology info:eu-repo/semantics/article Journal articles 2008 ftccsdartic https://doi.org/10.1111/j.1365-2052.2008.01776.x 2021-12-19T03:58:07Z International audience To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to ingestion than to absorption efficiency. Additionally, relative mRNA levels of the two amylase cDNAs were also strongly associated with amylase gene polymorphism, possibly reflecting variation in an undefined regulatory region, although no corresponding variation was observed in specific amylase activity. Amylase gene sequences were determined for each genotype, showing the existence of only synonymous or functionally equivalent non-synonymous polymorphisms. The observed associations among growth, food consumption-related traits and amylase gene polymorphism are therefore more likely to be related to variation in the level of amylase gene expression than to functional enzymatic variants. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Animal Genetics 39 6 662 665 |