Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system : effects of water on kinetic parameters
International audience The influence of water on the kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by immobilized lipase B from Candida antarctica was studied in a continuous solid/gas reactor. In this reactor, the solid phase is composed of a packed enzymatic sample which is...
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ftccsdartic:oai:HAL:hal-00329680v1 2023-05-15T13:51:27+02:00 Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system : effects of water on kinetic parameters Graber, Marianne Bousquet-Dubouch, Marie-Pierre Lamare, Sylvain Legoy, Marie-Dominique LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génie Protéique et Cellulaire (LGPC) Université de La Rochelle (ULR) 2003-01-14 https://hal.archives-ouvertes.fr/hal-00329680 https://hal.archives-ouvertes.fr/hal-00329680/document https://hal.archives-ouvertes.fr/hal-00329680/file/publi3.pdf en eng HAL CCSD Elsevier hal-00329680 https://hal.archives-ouvertes.fr/hal-00329680 https://hal.archives-ouvertes.fr/hal-00329680/document https://hal.archives-ouvertes.fr/hal-00329680/file/publi3.pdf info:eu-repo/semantics/OpenAccess ISSN: 1570-9639 Biochimica et Biophysica Acta Proteins and Proteomics https://hal.archives-ouvertes.fr/hal-00329680 Biochimica et Biophysica Acta Proteins and Proteomics, Elsevier, 2003, 1648, pp.24-32 [SDV.BIO]Life Sciences [q-bio]/Biotechnology [INFO.INFO-BT]Computer Science [cs]/Biotechnology info:eu-repo/semantics/article Journal articles 2003 ftccsdartic 2021-10-24T20:41:54Z International audience The influence of water on the kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by immobilized lipase B from Candida antarctica was studied in a continuous solid/gas reactor. In this reactor, the solid phase is composed of a packed enzymatic sample which is percolated by gaseous nitrogen, simultaneously carrying gaseous substrates to the enzyme while removing reaction products. In this system, interactions between the enzyme and non-reacting molecules are avoided, since no solvent is present, and it is thus more easy to assess the role of water. To this end, alcohol inhibition constant, substrates dissociation constants as well as acylation rate constant and ratio of acylation to deacylation rate constants have been determined as a function of water activity (aW). Data obtained highlight that n-propanol inhibition constant and dissociation constant of methyl propionate are a lot affected by aW variations whereas water has no significant effect on the catalytic acylation step nor on the ratio of acylation to deacylation rate constants. These results suggest the water independent character of the transition step. Article in Journal/Newspaper Antarc* Antarctica Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology [INFO.INFO-BT]Computer Science [cs]/Biotechnology |
spellingShingle |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology [INFO.INFO-BT]Computer Science [cs]/Biotechnology Graber, Marianne Bousquet-Dubouch, Marie-Pierre Lamare, Sylvain Legoy, Marie-Dominique Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system : effects of water on kinetic parameters |
topic_facet |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology [INFO.INFO-BT]Computer Science [cs]/Biotechnology |
description |
International audience The influence of water on the kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by immobilized lipase B from Candida antarctica was studied in a continuous solid/gas reactor. In this reactor, the solid phase is composed of a packed enzymatic sample which is percolated by gaseous nitrogen, simultaneously carrying gaseous substrates to the enzyme while removing reaction products. In this system, interactions between the enzyme and non-reacting molecules are avoided, since no solvent is present, and it is thus more easy to assess the role of water. To this end, alcohol inhibition constant, substrates dissociation constants as well as acylation rate constant and ratio of acylation to deacylation rate constants have been determined as a function of water activity (aW). Data obtained highlight that n-propanol inhibition constant and dissociation constant of methyl propionate are a lot affected by aW variations whereas water has no significant effect on the catalytic acylation step nor on the ratio of acylation to deacylation rate constants. These results suggest the water independent character of the transition step. |
author2 |
LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génie Protéique et Cellulaire (LGPC) Université de La Rochelle (ULR) |
format |
Article in Journal/Newspaper |
author |
Graber, Marianne Bousquet-Dubouch, Marie-Pierre Lamare, Sylvain Legoy, Marie-Dominique |
author_facet |
Graber, Marianne Bousquet-Dubouch, Marie-Pierre Lamare, Sylvain Legoy, Marie-Dominique |
author_sort |
Graber, Marianne |
title |
Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system : effects of water on kinetic parameters |
title_short |
Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system : effects of water on kinetic parameters |
title_full |
Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system : effects of water on kinetic parameters |
title_fullStr |
Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system : effects of water on kinetic parameters |
title_full_unstemmed |
Alcoholysis catalyzed by Candida antarctica lipase B in a gas/solid system : effects of water on kinetic parameters |
title_sort |
alcoholysis catalyzed by candida antarctica lipase b in a gas/solid system : effects of water on kinetic parameters |
publisher |
HAL CCSD |
publishDate |
2003 |
url |
https://hal.archives-ouvertes.fr/hal-00329680 https://hal.archives-ouvertes.fr/hal-00329680/document https://hal.archives-ouvertes.fr/hal-00329680/file/publi3.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ISSN: 1570-9639 Biochimica et Biophysica Acta Proteins and Proteomics https://hal.archives-ouvertes.fr/hal-00329680 Biochimica et Biophysica Acta Proteins and Proteomics, Elsevier, 2003, 1648, pp.24-32 |
op_relation |
hal-00329680 https://hal.archives-ouvertes.fr/hal-00329680 https://hal.archives-ouvertes.fr/hal-00329680/document https://hal.archives-ouvertes.fr/hal-00329680/file/publi3.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766255326975229952 |