Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas.
International audience For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of tri...
Published in: | Molecular Reproduction and Development |
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Main Authors: | , , , , , |
Other Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2006
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Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-00326597 https://doi.org/10.1002/mrd.20472 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
myotropin APGWamide egg‐laying molluscan mass spectrometry [CHIM.ORGA]Chemical Sciences/Organic chemistry |
spellingShingle |
myotropin APGWamide egg‐laying molluscan mass spectrometry [CHIM.ORGA]Chemical Sciences/Organic chemistry Bernay, Benoît Baudy-Floc'H, Michele Zanuttini, Bruno Zatylny, Céline Pouvreau, Stéphane Henry, Joël Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas. |
topic_facet |
myotropin APGWamide egg‐laying molluscan mass spectrometry [CHIM.ORGA]Chemical Sciences/Organic chemistry |
description |
International audience For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in vitro bioassay demonstrated that APGWamide modulates the AM contractions that insure the release of oocytes in the external medium. Exposure of oysters to a physiological concentration of APGWamide triggered repetitive shell closures. The second experimental approach was based on the monitoring of HPLC purification by a myotropic bioassay using the cuttlefish oviduct contractions as a target. The successive purification steps of the acidic extraction of ovaries from mature female oysters, led to the characterization of the hexapeptide PIESVD. When applied to mature female oysters, this peptide triggered the increase of shell closure frequency. The activity of these two regulatory peptides is the first experimental evidence of a peptidergic control of egg‐laying in oyster. APGWamide and PIESVD could be used, in commercial and experimental hatcheries, for the identification of mature females to be selected for in vitro fertilization. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Institut des Sciences Chimiques de Rennes (ISCR) Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA) Equipe MAD - Laboratoire GREYC - UMR6072 Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC) Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN) Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN) Normandie Université (NU) UMR100 Station Expérimentale d'Argenton Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Bernay, Benoît Baudy-Floc'H, Michele Zanuttini, Bruno Zatylny, Céline Pouvreau, Stéphane Henry, Joël |
author_facet |
Bernay, Benoît Baudy-Floc'H, Michele Zanuttini, Bruno Zatylny, Céline Pouvreau, Stéphane Henry, Joël |
author_sort |
Bernay, Benoît |
title |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas. |
title_short |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas. |
title_full |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas. |
title_fullStr |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas. |
title_full_unstemmed |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas. |
title_sort |
ovarian and sperm regulatory peptides regulate ovulation in the oyster crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.archives-ouvertes.fr/hal-00326597 https://doi.org/10.1002/mrd.20472 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1040-452X EISSN: 1098-2795 Molecular Reproduction and Development https://hal.archives-ouvertes.fr/hal-00326597 Molecular Reproduction and Development, Wiley, 2006, 73 (5), pp.607-616. ⟨10.1002/mrd.20472⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.20472 hal-00326597 https://hal.archives-ouvertes.fr/hal-00326597 doi:10.1002/mrd.20472 |
op_doi |
https://doi.org/10.1002/mrd.20472 |
container_title |
Molecular Reproduction and Development |
container_volume |
73 |
container_issue |
5 |
container_start_page |
607 |
op_container_end_page |
616 |
_version_ |
1766394642207604736 |
spelling |
ftccsdartic:oai:HAL:hal-00326597v1 2023-05-15T15:58:52+02:00 Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas. Bernay, Benoît Baudy-Floc'H, Michele Zanuttini, Bruno Zatylny, Céline Pouvreau, Stéphane Henry, Joël Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Institut des Sciences Chimiques de Rennes (ISCR) Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA) Equipe MAD - Laboratoire GREYC - UMR6072 Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC) Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN) Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN) Normandie Université (NU) UMR100 Station Expérimentale d'Argenton Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2006 https://hal.archives-ouvertes.fr/hal-00326597 https://doi.org/10.1002/mrd.20472 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.20472 hal-00326597 https://hal.archives-ouvertes.fr/hal-00326597 doi:10.1002/mrd.20472 ISSN: 1040-452X EISSN: 1098-2795 Molecular Reproduction and Development https://hal.archives-ouvertes.fr/hal-00326597 Molecular Reproduction and Development, Wiley, 2006, 73 (5), pp.607-616. ⟨10.1002/mrd.20472⟩ myotropin APGWamide egg‐laying molluscan mass spectrometry [CHIM.ORGA]Chemical Sciences/Organic chemistry info:eu-repo/semantics/article Journal articles 2006 ftccsdartic https://doi.org/10.1002/mrd.20472 2021-12-12T06:17:46Z International audience For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in vitro bioassay demonstrated that APGWamide modulates the AM contractions that insure the release of oocytes in the external medium. Exposure of oysters to a physiological concentration of APGWamide triggered repetitive shell closures. The second experimental approach was based on the monitoring of HPLC purification by a myotropic bioassay using the cuttlefish oviduct contractions as a target. The successive purification steps of the acidic extraction of ovaries from mature female oysters, led to the characterization of the hexapeptide PIESVD. When applied to mature female oysters, this peptide triggered the increase of shell closure frequency. The activity of these two regulatory peptides is the first experimental evidence of a peptidergic control of egg‐laying in oyster. APGWamide and PIESVD could be used, in commercial and experimental hatcheries, for the identification of mature females to be selected for in vitro fertilization. Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Molecular Reproduction and Development 73 5 607 616 |