Salmon HNF1: cDNA sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter.
cDNA clones coding for the transcription factor HNF1 have been isolated from Atlantic salmon (Salmo salar L.). The 559 amino acid residue long encoded protein shows high conservation, with respect to other species, of the domains necessary for DNA-binding: the HNF1 atypical homeodomain, the POU rela...
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Online Access: | https://hal.archives-ouvertes.fr/hal-00151186 https://doi.org/10.1006/jmbi.1994.0115 |
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ftccsdartic:oai:HAL:hal-00151186v1 2023-05-15T15:32:49+02:00 Salmon HNF1: cDNA sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter. Deryckere, F. Byrnes, L. Wagner, A. McMorrow, T. Gannon, F. Department of Microbiology (NATIONAL DIAGNOSTICS CENTRE) University College Galway 1995-03-17 https://hal.archives-ouvertes.fr/hal-00151186 https://doi.org/10.1006/jmbi.1994.0115 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1006/jmbi.1994.0115 info:eu-repo/semantics/altIdentifier/pmid/7897653 hal-00151186 https://hal.archives-ouvertes.fr/hal-00151186 doi:10.1006/jmbi.1994.0115 PUBMED: 7897653 ISSN: 0022-2836 EISSN: 1089-8638 Journal of Molecular Biology https://hal.archives-ouvertes.fr/hal-00151186 Journal of Molecular Biology, Elsevier, 1995, 247 (1), pp.1-10. ⟨10.1006/jmbi.1994.0115⟩ MESH: Amino Acid Sequence MESH: Animals MESH: Liver MESH: Molecular Sequence Data MESH: Nuclear Proteins MESH: Promoter Regions (Genetics) MESH: RNA Messenger MESH: Salmon MESH: Sequence Alignment MESH: Sequence Homology Amino Acid MESH: Serum Albumin MESH: Tissue Distribution MESH: Base Sequence MESH: Transcription Factors MESH: DNA Primers MESH: DNA Complementary MESH: DNA-Binding Proteins MESH: Evolution MESH: Gene Expression MESH: Hepatocyte Nuclear Factor 1 MESH: Hepatocyte Nuclear Factor 1-beta [SDV.BC]Life Sciences [q-bio]/Cellular Biology info:eu-repo/semantics/article Journal articles 1995 ftccsdartic https://doi.org/10.1006/jmbi.1994.0115 2020-12-26T17:59:32Z cDNA clones coding for the transcription factor HNF1 have been isolated from Atlantic salmon (Salmo salar L.). The 559 amino acid residue long encoded protein shows high conservation, with respect to other species, of the domains necessary for DNA-binding: the HNF1 atypical homeodomain, the POU related sequence and the dimerisation domain. Alignment with rat HNF1 protein reveals that the transcription activation domains ADI and ADIII are relatively conserved in the fish sequence whereas ADII is not. Phylogenetic analysis indicates that higher vertebrate HNF1s and the related variant HNF1s (vHNF1s) are more closely related to each other than any of them is to Salmon HNF1, suggesting that the duplication event from which HNF1 and vHNF1 genes arose occurred after the divergence of the tetrapod and teleost ancestors. Northern blot analysis show a single transcript, of about 2.6 kb, which is not exclusive to liver but is also present in intestine, kidney and spleen. Using polymerase chain reaction (PCR) we have isolated the salmon albumin gene promoter which contains, upstream of the TATA box, a potential binding site for HNF1. The salmon HNF1 protein synthesized by in vitro transcription-translation of the full-length cDNA is able to bind specifically with equivalent affinities to either the rat or salmon albumin promoter. Article in Journal/Newspaper Atlantic salmon Salmo salar Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Journal of Molecular Biology 247 1 1 10 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
MESH: Amino Acid Sequence MESH: Animals MESH: Liver MESH: Molecular Sequence Data MESH: Nuclear Proteins MESH: Promoter Regions (Genetics) MESH: RNA Messenger MESH: Salmon MESH: Sequence Alignment MESH: Sequence Homology Amino Acid MESH: Serum Albumin MESH: Tissue Distribution MESH: Base Sequence MESH: Transcription Factors MESH: DNA Primers MESH: DNA Complementary MESH: DNA-Binding Proteins MESH: Evolution MESH: Gene Expression MESH: Hepatocyte Nuclear Factor 1 MESH: Hepatocyte Nuclear Factor 1-beta [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
spellingShingle |
MESH: Amino Acid Sequence MESH: Animals MESH: Liver MESH: Molecular Sequence Data MESH: Nuclear Proteins MESH: Promoter Regions (Genetics) MESH: RNA Messenger MESH: Salmon MESH: Sequence Alignment MESH: Sequence Homology Amino Acid MESH: Serum Albumin MESH: Tissue Distribution MESH: Base Sequence MESH: Transcription Factors MESH: DNA Primers MESH: DNA Complementary MESH: DNA-Binding Proteins MESH: Evolution MESH: Gene Expression MESH: Hepatocyte Nuclear Factor 1 MESH: Hepatocyte Nuclear Factor 1-beta [SDV.BC]Life Sciences [q-bio]/Cellular Biology Deryckere, F. Byrnes, L. Wagner, A. McMorrow, T. Gannon, F. Salmon HNF1: cDNA sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter. |
topic_facet |
MESH: Amino Acid Sequence MESH: Animals MESH: Liver MESH: Molecular Sequence Data MESH: Nuclear Proteins MESH: Promoter Regions (Genetics) MESH: RNA Messenger MESH: Salmon MESH: Sequence Alignment MESH: Sequence Homology Amino Acid MESH: Serum Albumin MESH: Tissue Distribution MESH: Base Sequence MESH: Transcription Factors MESH: DNA Primers MESH: DNA Complementary MESH: DNA-Binding Proteins MESH: Evolution MESH: Gene Expression MESH: Hepatocyte Nuclear Factor 1 MESH: Hepatocyte Nuclear Factor 1-beta [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
description |
cDNA clones coding for the transcription factor HNF1 have been isolated from Atlantic salmon (Salmo salar L.). The 559 amino acid residue long encoded protein shows high conservation, with respect to other species, of the domains necessary for DNA-binding: the HNF1 atypical homeodomain, the POU related sequence and the dimerisation domain. Alignment with rat HNF1 protein reveals that the transcription activation domains ADI and ADIII are relatively conserved in the fish sequence whereas ADII is not. Phylogenetic analysis indicates that higher vertebrate HNF1s and the related variant HNF1s (vHNF1s) are more closely related to each other than any of them is to Salmon HNF1, suggesting that the duplication event from which HNF1 and vHNF1 genes arose occurred after the divergence of the tetrapod and teleost ancestors. Northern blot analysis show a single transcript, of about 2.6 kb, which is not exclusive to liver but is also present in intestine, kidney and spleen. Using polymerase chain reaction (PCR) we have isolated the salmon albumin gene promoter which contains, upstream of the TATA box, a potential binding site for HNF1. The salmon HNF1 protein synthesized by in vitro transcription-translation of the full-length cDNA is able to bind specifically with equivalent affinities to either the rat or salmon albumin promoter. |
author2 |
Department of Microbiology (NATIONAL DIAGNOSTICS CENTRE) University College Galway |
format |
Article in Journal/Newspaper |
author |
Deryckere, F. Byrnes, L. Wagner, A. McMorrow, T. Gannon, F. |
author_facet |
Deryckere, F. Byrnes, L. Wagner, A. McMorrow, T. Gannon, F. |
author_sort |
Deryckere, F. |
title |
Salmon HNF1: cDNA sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter. |
title_short |
Salmon HNF1: cDNA sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter. |
title_full |
Salmon HNF1: cDNA sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter. |
title_fullStr |
Salmon HNF1: cDNA sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter. |
title_full_unstemmed |
Salmon HNF1: cDNA sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter. |
title_sort |
salmon hnf1: cdna sequence, evolution, tissue specificity and binding to the salmon serum albumin promoter. |
publisher |
HAL CCSD |
publishDate |
1995 |
url |
https://hal.archives-ouvertes.fr/hal-00151186 https://doi.org/10.1006/jmbi.1994.0115 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 0022-2836 EISSN: 1089-8638 Journal of Molecular Biology https://hal.archives-ouvertes.fr/hal-00151186 Journal of Molecular Biology, Elsevier, 1995, 247 (1), pp.1-10. ⟨10.1006/jmbi.1994.0115⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1006/jmbi.1994.0115 info:eu-repo/semantics/altIdentifier/pmid/7897653 hal-00151186 https://hal.archives-ouvertes.fr/hal-00151186 doi:10.1006/jmbi.1994.0115 PUBMED: 7897653 |
op_doi |
https://doi.org/10.1006/jmbi.1994.0115 |
container_title |
Journal of Molecular Biology |
container_volume |
247 |
container_issue |
1 |
container_start_page |
1 |
op_container_end_page |
10 |
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1766363298602680320 |